5-TAMRA CAS 91809-66-4 Purity >97.0% (HPLC at 254nm)

Short Description:

Chemical Name: 5-Carboxytetramethylrhodamine

Synonyms: 5-TAMRA

CAS: 91809-66-4

Purity: >97.0% (HPLC at 254nm) 

Appearance: Red to Dark Purple Powder to Soild

Fluorescent Dye for DNA, Peptides and Proteins

E-Mail: alvin@ruifuchem.com


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Description:

Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of 5-Carboxytetramethylrhodamine, 5-TAMRA (CAS: 91809-66-4) with high quality. We can provide COA, worldwide delivery, small and bulk quantities available. Please contact: alvin@ruifuchem.com

Chemical Properties:

Chemical Name 5-Carboxytetramethylrhodamine
Synonyms 5-TAMRA 
CAS Number 91809-66-4
CAT Number RF-PI2188
Stock Status In Stock, Production Scale Up to Tons
Molecular Formula C25H22N2O5   
Molecular Weight 430.45
Spectral Properties Excitation Wavelength: 552 nm
Emission Wavelength: 578 nm
Solubility Soluble In DMSO
Source Synthetic
Category Fluorescent Reagents
Brand Ruifu Chemical

Specifications:

Item Specifications
Appearance Red to Dark Purple Powder to Soild (Visual Inspection)
Identity 1H  NMR, MS Conforms
Purity / Analysis Method >97.0% (HPLC at 254nm)
Column: XDB-C18
Flow Rate: 0.8mL/min
Detection: UV at 254nm
HPLC Elution Buffer: CH3CN:TFA (0.1%)=35:65
Test Standard Enterprise Standard
Shelf Life 12 Months From Manufacture Date if Stored Properly 

Package & Storage:

Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement

Storage Condition: Store in sealed containers at cool and dry place, Freeze (<-15℃), Minimize light exposure, Keep in dark place, Inert atmosphe, Protect from moisture

Advantages:

1

FAQ:

Application:

5-Carboxytetramethylrhodamine, 5-TAMRA (CAS: 91809-66-4), is a long-wavelength dye, fluorescent dye for DNA, peptides and proteins. Amine-reactive. Used for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore. Free 5(6)-TAMRA (ab145282) and 6-TAMRA (ab145338) isomer. 5-TAMRA N-succinimidyl ester (ab145433) precursor. 5-TAMRA, SE and its conjugates yield bright, pH-insensitive orange-red fluorescence with good photostability. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is widely used to label peptides and proteins. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates. Currently AAT Bioquest is using a proprietary process to synthesize isomer-free 5-TAMRA without the conventional chromatographic purification.

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