Bis-Tris CAS 6976-37-0 Purity >99.0% (Titration) Biological Buffer Ultra Pure
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Chemical Name | Bis-Tris |
Synonyms | Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane; Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane; 2,2-Bis(hydroxymethyl)-2,2′,2''-Nitrilotriethanol; Bis-Tris Methane |
CAS Number | 6976-37-0 |
CAT Number | RF-PI1653 |
Stock Status | In Stock, Production Scale Up to Tons |
Molecular Formula | C8H19NO5 |
Molecular Weight | 209.24 |
Brand | Ruifu Chemical |
Item | Specifications |
Grade | Ultra Pure |
Appearance | White Crystalline Powder |
Purity / Analysis Method | >99.0% (Titration on Dried Basis) |
Melting Point | 102.0~106.0℃ |
Water (by Karl Fischer) | <0.50% |
Loss on Drying | <0.50% 20 ℃ (HV) |
Residue on Ignition | <0.20% |
Insoluble Matter | Passes Filter Test |
Heavy Metals (as Pb) | ≤5ppm |
Iron (Fe) | ≤5ppm |
UV A280nm | ≤0.04 (1 cm/0.1 M in Water) |
UV A340nm | ≤0.02 (1 cm/0.1 M in Water) |
pH | 9.5~11.0 (1.0M aqueous Solution at 25℃) |
Useful pH Range | 5.8~7.2 |
pKa (25℃) | 6.45~6.65 |
Solubility | Clear and Complete (0.1M in H2O) |
Chloride (Cl-) | ≤50 mg/kg |
Sulfate (SO42-) | ≤50 mg/kg |
Al | ≤5 mg/kg |
As | ≤0.1 mg/kg |
Ba | ≤5 mg/kg |
Bi | ≤5 mg/kg |
Ca | ≤10 mg/kg |
Cd | ≤5 mg/kg |
Co | ≤5 mg/kg |
Cr | ≤5 mg/kg |
Cu | ≤5 mg/kg |
Iron (Fe) | ≤5 mg/kg |
K | ≤50 mg/kg |
Li | ≤5 mg/kg |
Mg | ≤5 mg/kg |
Mn | ≤5 mg/kg |
Mo | ≤5 mg/kg |
Na | ≤50 mg/kg |
Ni | ≤5 mg/kg |
Sr | ≤5 mg/kg |
Infrared Spectrum | Conforms to Structure |
Test Standard | Enterprise Standard |
Usage | Biological Buffer; Good's Buffer Component for Biological Research |
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement.
Storage Condition: Store in sealed containers at cool and dry place; Protect from light and moisture.
Bis-Tris (CAS 618-89-3) is a commonly used biological buffer, used in biochemistry and molecular biology. Bis-Tris is an inert, zwitterionic buffer that exhibits only a very small shift in dissociation constant. It is a bis(2-hydroxyethyl)amine and Tris family buffer. Bis-Tris is of an Ultra Pure Grade. Bis-tris methane is an organic tertiary amine with labile protons having a pKa of 6.46 at 25℃. It is an effective buffer between the pH 5.8~7.2. Bis-Tris binds strongly to Cu and Pb ions as well as, weakly, to Mg, Ca, Mn, Co, Ni, Zn and Cd. Bis-Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing.