Adenosine CAS 58-61-7 Assay 99.0% -101.0% USP Standard Factory High Purity

BAYANI
Adenosine ya ƙunshi NLT 99.0% da NMT 101.0% na C10H13N5O4, ƙididdiga akan busasshen tushe.
GANO
• A. RASHIN NUTSUWA <197K>: NMT 0.1%
ASSAY
• ADENOSINE
Misali: 200 MG na Adenosine a baya ya bushe a 105 ° don 2h
Titrimetric tsarin
(Duba Titrimetry <541>)
Yanayin: kai tsaye titration
Titrant: 0.1 N kowane chloric acid VS
Gano ƙarshen ƙarshen: Potentiometric
Analysis: Narke a cikin 50 ml na glacial acetic acid da titrate tare da 0.1 N da chloric acid VS.Yi lissafin kashi na Adenosine (C10H13N5O4) a cikin ɓangaren da aka ɗauka:
Sakamako = [(V - B) × N × F × 100]/W
V = Samfurin titrant girma (mL)
B = Ƙarar titrant (mL)
N = daidaitaccen titrant (mEq/ml)
F= Matsakaicin daidai: 267.25 mg/mEq
W= Nauyin Samfurin (mg)
Sharuɗɗan karɓa: 99.0% -101.0% akan busasshen tushe
• RASHIN ZUCIYA
• RASHIN WUTA <281>: NMT 0.1%
• KARFE MAI KYAU, Hanyar II <231>: NMT 10 ppm
• IYAKA NA Ammoniya
Maganin samfurin: Dakatar da 0.5 g a cikin 10 ml na ruwa.Dama na tsawon s30, kuma ku wuce ta cikin babban tacewa.Tsarma da tacewa da ruwa zuwa 15 ml, kuma amfani da tacewa.
Daidaitaccen bayani: 0.4 µg/mL na ammonium chloride a cikin ruwa
Analysis: Zuwa Maganin Samfurin da daidaitaccen bayani ƙara 0.3 ml na alkaline mer curic–potassium iodide TS, hular bututun gwaji, kuma ba da damar tsayawa na 5 min.
Sharuɗɗan karɓa: Maganin Samfurin baya nuna tsananin launin rawaya fiye da na daidaitaccen bayani (NMT 4 ppm na ammonia).
• IYAKA NA chloride
Maganin samfurin: Dakatar da 0.2 g a cikin 10 ml na ruwa.Dama har tsawon 30s, wuce ta cikin babban tacewa, kuma amfani da tacewa.
Daidaitaccen bayani: 2.3 µg/mL na sodium chloride a cikin ruwa
Analysis: Zuwa samfurin samfurin da 10 ml na daidaitaccen bayani ƙara 1 ml na nitric acid da 1 ml na nitrate na azurfa TS, kuma a tsoma kowane bayani da ruwa zuwa 40 ml.Bada mafita don tsayawa na 5 min, an kare shi daga haske.
Sharuɗɗan karɓa: Lokacin da aka duba shi da bango mai duhu, Samfurin mafita bai fi turbid fiye da daidaitaccen bayani ba (NMT 0.007% chloride).
• IYAKA SULFATE
Maganin samfurin: Dakatar da 0.75 g a cikin 15 ml na ruwa.Dama har tsawon 30s, wuce ta cikin babban tacewa, kuma amfani da tacewa.
Daidaitaccen bayani: Ƙara 0.15 ml na 0.020 N sulfuric acid zuwa 15 ml na ruwa.
Analysis: Zuwa Samfurin bayani da daidaitaccen bayani ƙara 2 ml na barium chloride TS da 1 ml na 3 N hydrochloric acid, tsarma kowane bayani da ruwa zuwa 30 ml, da haɗuwa.Bada mafita su tsaya na minti 5.
Sharuɗɗan karɓa: Maganin Samfurin bai fi turbid fiye da daidaitaccen bayani (NMT 0.02% sulfate).
• RASHIN GIRMA
Magani A: 6.8 g/L na potassium hydrogen sulfate da 3.4g/L na tetrabutylammonium hydrogen sulfate a cikin ruwa.Daidaita tare da 2 N potassium hydroxide zuwa pH na 6.5.
Magani B: 0.1 g/L na maganin sodium azide
Zaman Waya: Magani A da Magani B (60:40)
Maganin dacewa da tsarin: 0.2 mg/mL kowanne na Adenosine da inosine a cikin Wayar hannu
Maganin samfurin: 1.0 mg/mL na Adenosine a cikin Wayar hannu
Tsarin chromatographic
(Duba Chromatography <621>, Dacewar Tsarin.)
Yanayin: LC
Mai ganowa: UV 254 nm
Tushen: 4.6-mm × 25-cm;5-µm shiryarwa L1
Yawan gudu: 1.5 ml/min
Girman allura: 20µL
Dacewar tsarin
Samfurori: Maganin dacewa da tsarin
Abubuwan da suka dace
Resolution: NLT 9.0 tsakanin adenosine da inosine
Matsayin wutsiya: NMT 2.5
Matsakaicin madaidaicin dangi: NMT 2.0%
[NOTE-Chromatograph da Samfurin bayani, kuma daidaita lokacin gudu zuwa aƙalla sau biyu lokacin riƙewar babban kololuwa.]
Nazari
Misali: Misalin bayani
Yi ƙididdige adadin kowane ƙazanta a cikin ɓangaren Adenosine da aka ɗauka:
Sakamako = (rU/rT) × 100
rU = kololuwar martani na kowane kazanta daga Maganin Samfurin
rT = jimlar duk martani ga duk kololuwa daga Maganin Samfurin
Sharuɗɗan karɓa
Najasa ɗaya: NMT 0.1% kowanne na guanosine, inosine, da uridine, da NMT 0.2% na adenine
Jimlar ƙazanta: NMT 0.5%
MAKAMMAN JARRABAWA
• MATSAYIN NARKI KO WUYA <741>: 233°-238°
• Juyawa na gani, Jujjuya ta musamman <781S>: -68° zuwa -72°
Maganin gwaji: 20 mg / mL a cikin maganin sodium hydroxide (1 a cikin 20), ƙaddara akan samfurin da aka bushe a baya a 105 ° don 2h
ACIDITY KO ALKALINITY: Dakatar da 1 g a cikin 20 ml na ruwa mara kyau na carbon dioxide.Dama har tsawon 30s, kuma ku wuce ta cikin babban tacewa.Ga kowane kashi biyu na 10-ml na tacewa ƙara 0.1mL na bromocresol purple TS.
Sharuɗɗan karɓa: Ana buƙatar NMT 0.3 ml na 0.01 N sodium hydroxide don samar da launin shuɗi-violet a cikin yanki ɗaya.Ana buƙatar NMT 0.1 ml na 0.01 N hydrochloric acid don samar da launin rawaya a ɗayan ɓangaren.
BUSHEN RASHI <731>: bushe samfurin a 105 ℃ na awa 2: yana rasa NMT 0.5% na nauyinsa.
KARIN BUKATA
• MAKIYAYYA DA AJIYA: Ajiye a cikin matsuguni, kwantena masu jure haske, da adanawa a yanayin zafin ɗaki mai sarrafawa.
• MATSAYIN NAZARI NA USP <11>
USP Adenosin RS