Bacitracin Zinc CAS 1405-89-6 Ƙarfin ≥70 IU/MG Kamfanin Magungunan rigakafi na Peptide

Bacitracins, hadadden zinc.
Bacitracins zinc hadaddun [1405-89-6].
» Bacitracin Zinc shine hadadden zinc na bacitracin, wanda ya ƙunshi cakuda polypeptides na antimicrobial, babban abubuwan da aka haɗa shine bacitracins A, B1, B2, da B3.Yana da ƙarfin da bai gaza 65 Bacitracin Units a kowace MG ba, ƙididdiga akan busasshen tushe.Ya ƙunshi bai wuce kashi 4.0 ba kuma bai wuce kashi 6.0 na zinc (Zn), wanda aka ƙididdige shi akan busasshiyar tushe.
Marufi da ajiya - Ajiye a cikin matsuguni, kuma adana a wuri mai sanyi.
Lakabi-Lakafta shi don nuna cewa za a yi amfani da shi wajen kera magungunan marasa iyaye kawai.Inda aka tattara ta don haɗa magunguna, sanya alama don nuna cewa ba ta da haihuwa kuma ba za a iya tabbatar da ƙarfin ba fiye da kwanaki 60 bayan buɗewa, kuma a faɗi adadin Bacitracin Units a kowace milligram.Inda aka yi niyya don amfani da shi wajen shirya nau'ikan allurai na bakararre, alamar ta bayyana cewa ba ta da lafiya ko kuma dole ne a ci gaba da aiki da ita yayin shirye-shiryen fom ɗin allurai.
Matsayin Magana na USP <11>-
USP Bacitracin Zinc RS
Ganewa-
A: Gwajin Shaida na Chromatographic Na Bakin Bakin Ciki <201BNP>: ya cika buƙatun.
B: Ya dace da buƙatun tsarin chromatographic ruwa a cikin gwaji don Haɗa.
Sterility <71> - Inda alamar ta bayyana cewa ba ta da lafiya, ta cika buƙatun lokacin da aka gwada ta kamar yadda aka umarce ta don Tacewar Membrane a ƙarƙashin Gwajin Rashin Haɗuwar Samfurin da za a gwada, sai dai don amfani da Fluid A ga kowane L wanda aka ƙara 20 g na edetate disodium.
pH <791>: tsakanin 6.0 da 7.5, a cikin (cikakken) bayani wanda ya ƙunshi kusan 100 MG a kowace ml.
Asarar bushewa <731> - bushe game da 100 MG a cikin kwalban da aka dakatar da capillary a cikin injin a cikin 60 na sa'o'i 3: ya rasa fiye da 5.0% na nauyinsa.
Abubuwan da ke cikin Zinc- [bayanin kula-Tsarin shirye-shiryen da gwajin gwajin ƙila za a iya diluted da yawa tare da 0.001 N hydrochloric acid, idan ya cancanta, don samun mafita na abubuwan da suka dace, masu daidaitawa zuwa layin layi ko aiki na kayan aiki.]
Shirye-shiryen daidaitattun - Canja wurin 3.11 g na zinc oxide, daidaitattun aunawa, zuwa 250-mL volumetric flask, ƙara 80 ml na 1 N hydrochloric acid, dumi don narkewa, sanyi, tsarma da ruwa zuwa ƙarar, da haɗuwa.Wannan bayani ya ƙunshi 10 MG na zinc a kowace ml.Kara tsarma wannan bayani tare da 0.001 N hydrochloric acid don samun daidaitattun shirye-shirye dauke da 0.5, 1.5, da 2.5 µg na zinc a kowace ml, bi da bi.
Shirye-shiryen gwaji-Canja wurin kusan 200 MG na Zinc na Bacitracin, daidai a auna, zuwa filasta na 100-ml 100.Narke a cikin 0.01 N hydrochloric acid, tsoma tare da sauran ƙarfi iri ɗaya zuwa ƙara, da haɗuwa.Pipet 2 ml na wannan maganin a cikin kwalban 200-mL volumetric flask, tsarma da 0.001 N hydrochloric acid zuwa girma, da kuma Mix.
Tsari-Concomitantly ƙayyade absorbances na Standard shirye-shirye da Gwaji shiri a tutiya resonance line na 213.8 nm, tare da dace atomic sha spectrophotometer (duba Spectrophotometry da Haske-watsawa <851>), sanye take da tutiya m-cathode fitila da kuma harshen wuta-acetylene, ta amfani da 0.001 N hydrochloric acid a matsayin blank.Shirya abubuwan sha na daidaitattun shirye-shirye tare da maida hankali, a cikin µg kowace ml, na zinc, kuma zana layin madaidaiciya mafi dacewa da maki uku da aka ƙulla.Daga jadawali da aka samu, ƙayyade ƙaddamarwa, a cikin µg kowace ml, na zinc a cikin gwajin gwajin.Yi lissafin abubuwan da ke cikin zinc, a cikin kashi, a cikin ɓangaren Bacitracin Zinc da aka ɗauka ta dabara:
1000C / W
wanda C shine maida hankali a cikin µg a kowace ml, na zinc a cikin gwajin gwajin;kuma W shine nauyin, a cikin MG, na ɓangaren Bacitracin Zinc da aka ɗauka.
Abun ciki-
Buffer-Narke 34.8 g na potassium phosphate, dibasic, a cikin 1 L na ruwa.Daidaita tare da 27.2 g na potassium phosphate, monobasic, narkar da a cikin 1 L na ruwa, zuwa pH na 6.0.
Wayar hannu Phase-Shirya cakuda methanol, ruwa, Buffer, da acetonitrile (26:15:5:2).Mix da kyau, da kuma degas.
Diluent-Narke 40 g na edetate disodium a cikin 1 L na ruwa.Daidaita tare da tsarma sodium hydroxide zuwa pH na 7.0.
Maganin dacewa da tsarin-Narkar da adadin USP Bacitracin Zinc RS daidai gwargwado a cikin Diluent don samun mafita tare da ƙarancin ƙima na kusan 2.0 MG kowace ml.
Ba da rahoto ga bakin kofa-Rarraba ƙididdigewa, tare da ruwa, ƙarar da ta dace na tsarin dacewa da tsarin don samun bayani tare da sanannen taro na 0.01 MG kowace ml.
Maganin gano kololuwa-Narke adadin USP Bacitracin Zinc RS mai aunawa a cikin madaidaicin ƙarar Diluent don samun mafita tare da ƙima na kusan 2.0 MG kowace ml.Gasa a cikin ruwan zãfi na wanka na minti 30.Sanyi zuwa zafin jiki.
Maganin Gwaji-Narke daidai gwargwado na Bacitracin Zinc a cikin Diluent don samun mafita tare da ƙarancin ƙima na kusan 2.0 MG kowace ml.
Tsarin Chromatographic (duba Chromatography <621>) -Chromatograph na ruwa yana sanye da na'urar gano abin sha da ginshiƙin 4.6- × 250-mm mai ƙarewa wanda ya ƙunshi 5-µm tattara L1.Adadin kwarara shine 1.0 ml a minti daya.Saita tsawon zangon mai ganowa a 300 nm.Allurar kusan 100 µL na maganin gano Peak, kuma gano wurin bacitracin F, wanda sanannen ƙazanta ne, ta amfani da lokacin riƙewar dangi da aka nuna a cikin Tebura 1.
Tebur 1
Sunan Bangaren Lokacin Riƙewa Dangi (kimanin)
Bacitracin C1 0.5
Bacitracin C2 0.6
Bacitracin C3 0.6
Bacitracin B1 0.7
Bacitracin B2 0.7
Bacitracin B3 0.8
Bacitracin A 1.0
Bacitracin F 2.4
Canja tsawon zangon mai ganowa kuma saita shi zuwa 254 nm.Chromatograph da tsarin dacewa da tsarin, da yin rikodin martanin kololuwa kamar yadda aka umurce su don Tsara: gano kololuwar abubuwan da suka fi aiki na bacitracin (bacitracins A, B1, B2 da B3), peptides na farko (waɗanda ke fitowa a gaban kololuwa saboda bacitracin B1). ) da ƙazanta, bacitracin F, ta yin amfani da ƙimar lokacin riƙewar dangi da aka bayar a cikin Tebur 1. Yi ƙididdige ƙimar kololuwa zuwa kwari ta amfani da dabara:
Hp / H
wanda Hp shine tsayi sama da tushe na kololuwa saboda bacitracin B1;kuma HV shine tsayi sama da tushe na mafi ƙasƙanci mafi ƙasƙanci na lanƙwasa da ke raba bacitracin B1 ganiya daga kololuwa saboda bacitracin B2.Matsakaicin kololuwa zuwa kwari bai gaza 1.2 ba.
Tsari-Bayan allura daidai juzu'i (100 µL) na Diluent, Maganin Gwaji, da Maganin Bayar da Rahoto.Yi rikodin chromatogram na kusan sau uku lokacin riƙewa na bacitracin A. Gano kololuwa ta amfani da lokutan riƙewa da aka nuna a cikin Tebu 1. Auna kololuwar duk kololuwa a cikin maganin gwajin.[bayanin kula-Kin kula da duk wani kololuwa a cikin maganin Gwajin yana da yanki ƙasa da yankin bacitracin.yi watsi da duk wani kololuwar da aka gani a cikin Diluent.]
bayanin kula-Jimlar yanki a cikin lissafin masu zuwa an ayyana shi azaman yanki na duk kololuwa ban da iyakar bayar da rahoto.
Abubuwan da ke cikin bacitracin a-Kirga adadin bacitracin A ta amfani da dabara:
(rA/Jimlar yanki) × 100
A cikin abin da rA ke da martanin yanki daga bacitracin A. Bacitracin A abun ciki bai ƙasa da 40.0% na jimlar yanki ba.
abun ciki na bacitracin mai aiki-Kirga adadin bacitracin mai aiki (bacitracin A, B1, B2, da B3) ta amfani da dabara:
(rA + rB1 + rB2 + rB3 / Jimlar yanki) × 100
a cikin wanda rA, rB1, rB2, da rB3 sune martanin yanki daga bacitracin A, B1, B2, da B3, bi da bi.Jimlar bacitracin A, B1, B2, da B3 bai gaza 70.0% na jimlar yanki ba.
Iyakar farkon eluting peptides-Kirga adadin duk kololuwar da ke sama kafin kololuwa saboda bacitracin B1 ta amfani da dabara:
(rPreB1 / Jimlar yanki) ×100
a cikin wanda rPreB1 shine jimillar martanin duk kololuwa da ke fitowa kafin kololuwar bacitracin B1.Iyakar peptides na farko (waɗanda ke tashi a gaban kololuwa saboda bacitracin B1) bai wuce 20.0%.
Iyakar bacitracin f- Yi lissafin adadin bacitracin F ta amfani da dabara:
100 × (rF / rA)
wanda rF shine martanin bacitracin F daga maganin gwajin;kuma rA shine martanin bacitracin A daga maganin gwajin.Iyakar bacitracin F, sanannen ƙazanta, bai wuce 6.0% ba.
Assay-Ci gaba tare da Bacitracin Zinc kamar yadda aka ba da umarni a ƙarƙashin Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararru 81.