Chenodeoxycholic Acid (CDCA) CAS 474-25-9 Assay ≥98% (Dry Basic)

Chenodeoxycholic acid
C24H40O4: 392.57
3a,7a-Dihydroxy-5b-cholan-24-oic acid [474-25-9]
Chenodeoxycholic acid, lokacin da aka bushe, ya ƙunshi bai wuce 98.0z ba kuma bai wuce 101.0% na C24H40O4 ba.
Bayanin Chenodeoxycholic Acid yana faruwa azaman fari, lu'ulu'u, foda ko foda.
Yana narkewa da yardar kaina a cikin methanol kuma a cikin ethanol (99.5), mai narkewa a cikin acetone, kuma a zahiri baya narkewa cikin ruwa.
Gane Ƙayyadaddun bakan infrared na Chenodeoxycholic Acid, wanda aka bushe a baya, kamar yadda aka umarce shi a cikin hanyar faifan faifan potassium bromide a ƙarƙashin Infrared Spectrophotometry <2.25>, kuma kwatanta bakan tare da Spectrum Reference: duka bakan suna nuna irin ƙarfin sha a lambobi iri ɗaya.
Juyawar gani <2.49> [a] 20D: +11.0 °~ +13.0° (bayan bushewa, 0.4 g, ethanol (99.5), 20 ml, 100 mm.
Matsayin narkewa <2.60> 164 ~ 169 ℃
Tsarkake (1) Chloride <1.03>-Narke 0.36 g na Chenodeoxycholic Acid a cikin 30 ml na methanol, ƙara 10 ml na nitric acid dilute da ruwa don yin 50 ml, sannan a yi gwajin tare da wannan maganin.Shirya maganin kulawa kamar haka: Zuwa 1.0 ml na 0.01 mol / L hydrochloric acid VS ƙara 30 ml na methanol, 10 ml na dilute nitric acid da ruwa don yin 50 ml (ba fiye da 0.1%) ba.
(2) Karfe masu nauyi <1.07>- Ci gaba da 1.0 g na Chenodeoxycholic Acid bisa ga Hanyar 4, kuma yi gwajin.Shirya maganin sarrafawa tare da 2.0 ml na Maganin Jagorar Jagora (ba fiye da 20 ppm ba).
(3) Barium-zuwa 2.0 g na Chenodeoxycholic Acid ƙara 100 ml na ruwa, kuma tafasa don minti 2.A cikin wannan maganin ƙara 2 ml na hydrochloric acid, tafasa don minti 2, tace bayan sanyi, kuma wanke tace da ruwa har sai an sami 100 ml na tacewa.Zuwa 10 ml na tacewa ƙara 1 ml na tsarma sulfuric acid: babu turbidity ya bayyana.
(4) Abubuwan da ke da alaƙa-Narke 0.20 g na Chenodeoxycholic Acid a cikin cakuda acetone da ruwa (9: 1) don yin daidai 10 ml, kuma amfani da wannan maganin azaman maganin samfurin.Na dabam, narke 10 MG na lithocholic acid don chromatography na bakin ciki a cikin cakuda acetone da ruwa (9: 1) don yin daidai 10 ml.Pipet 2 ml na wannan bayani, ƙara cakuda acetone da ruwa (9: 1) don yin daidai 100 ml, kuma amfani da wannan bayani azaman daidaitaccen bayani (1).Na dabam, narke 10 MG na ursodeoxycholic acid a cikin cakuda acetone da ruwa (9: 1) don yin daidai 100 ml, kuma yi amfani da wannan bayani a matsayin daidaitaccen bayani (2).Na dabam, narke 10 MG na cholic acid don chromatography na bakin ciki a cikin cakuda acetone da ruwa (9: 1) don yin daidai 100 ml, kuma yi amfani da wannan bayani a matsayin daidaitaccen bayani (3).Pipet 1 ml na maganin samfurin, kuma ƙara cakuda acetone da ruwa (9: 1) don yin daidai 20 ml.Pipet 0.5 ml, 1 ml, 2 ml, 3 ml da 5 ml na wannan maganin, ƙara cakuda acetone da ruwa (1) ga kowannensu don yin daidai 50 ml, kuma sanya waɗannan mafita azaman daidaitaccen bayani A, daidaitaccen bayani. B, daidaitaccen bayani C, daidaitaccen bayani D da daidaitaccen bayani E, bi da bi.Yi gwajin tare da waɗannan mafita kamar yadda aka umurce su a ƙarƙashin Chromatography na Thin-Layer<2.03>Spot 5 ml kowane samfurin bayani, daidaitaccen mafita (1), (2), (3) da daidaitattun mafita A, B, C, D da E akan farantin gel na silica don chromatography na bakin ciki.Ci gaba da farantin tare da cakuda 4-methyl-2-pentanone, toluene da formic acid (16: 6: 1) zuwa nisa na kimanin 15 cm, bushe farantin, kuma ƙara bushe a 1209C na minti 30.Nan da nan, fesa daidai da bayani na phosphomolybdic acid n-hydrate a cikin ethanol (95) (1 cikin 5) akan farantin, kuma zafi a 120 ℃ na minti 2 zuwa 3: wurin da ya dace da tabo tare da daidaitaccen bayani (1) bai fi tabo mai daidaitaccen bayani ba (1), wurin da ya dace da tabo tare da daidaitaccen bayani (2) bai fi tabo mai daidaitaccen bayani ba (2), da tabo daidai da tabo. tare da daidaitaccen bayani (3) bai fi tsanani fiye da tabo tare da daidaitaccen bayani ba
(3).Kamar yadda idan aka kwatanta da spots tare da daidaitattun mafita A, B, C, D da E, aibobi ban da babban tabo da sauran abubuwan da aka ambata a sama ba su da ƙarfi fiye da tabo tare da daidaitaccen bayani E, da jimlar adadin. daga cikinsu bai wuce 1.5% ba.
Asarar bushewa <2.41> Ba fiye da 1.5% (1 g, 105 ℃, 3 hours).
Ragowa akan kunnawa <2.44> Bai wuce 0.1% ba (1 g).
Assay Yi nauyi daidai game da 0.5 g na Chenodeoxycholic Acid, wanda aka bushe a baya, narke a cikin 40 ml na ethanol (95) da 20 ml na ruwa, da titrate <2.50> tare da 0.1 mol/L sodium hydroxide VS (titration mai ƙarfi).Yi ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun tsari, da yin duk wani gyara da ya dace.
Kowane ml na 0.1 mol/L sodium hydroxide VS ＝ 39.26 MG na C24H40O4
Kwantena da Akwatunan ajiya - Kwantena masu tsauri.