Chloramphenicol CAS 56-75-7 Tsafta ≥99.0% (HPLC) Babban Tsabta

Chloramphenicol
C11H12Cl2N2O5 323.13 [56-75-7].
Chloramphenicol ya ƙunshi bai wuce kashi 97.0 ba kuma bai wuce kashi 103.0 na C11H12Cl2N2O5 ba.
Marufi da ajiya-Ajiye a cikin matsuguni.
Lakabi-Inda aka yi niyya don amfani da shi wajen shirya nau'ikan allura ko wasu nau'ikan alluran bakararre, alamar ta bayyana cewa ba ta da lafiya ko kuma dole ne a ƙara sarrafa ta yayin shirye-shiryen alluran allura ko wasu nau'ikan alluran bakararre.
Matsayin Magana na USP <11>-
USP Chloramphenicol RS Danna don Duba Tsarin
USP Endotoxin RS
Ganewa-
A: Ciwon Infrared <197K>.
B: Lokacin riƙewa na babban kololuwa a cikin chromatogram na shirye-shiryen Assay ya dace da abin da ke cikin chromatogram na daidaitaccen shiri, kamar yadda aka samu a cikin Assay.
Kewar narkewa <741>: tsakanin 149 da 153.
Takamaiman juyawa <781S>: tsakanin +17.0 da +20.0.
Maganin gwaji: 50 MG, ba a bushe ba, kowace ml, a cikin barasa mai bushewa.
Crystallinity <695>: ya dace da buƙatun.
Bacterial endotoxins 85-Inda aka yi nufin amfani da Chloramphenicol don shirya nau'ikan alluran allura, yana ƙunshe da bai wuce 0.2 USP Endotoxin Unit da MG na chloramphenicol ba.
Haihuwa 71- Inda alamar ta bayyana cewa Chloramphenicol ba ta da lafiya, ta cika buƙatun lokacin da aka gwada ta kamar yadda aka umurce ta don Tacewar Membrane a ƙarƙashin Gwajin Haihuwar Samfur don a gwada, sai dai don amfani da 1 g na ƙaƙƙarfan samfur.
pH <791>: tsakanin 4.5 da 7.5, a cikin dakatarwar ruwa mai ɗauke da 25 MG kowace ml.
Tsaftar Chromatographic-Narkar da adadin Chloramphenicol daidai gwargwado a cikin methanol don samun maganin gwaji mai ɗauke da MG 10 a kowace ml.Shirya maganin USP Chloramphenicol RS a cikin methanol mai dauke da 10 MG kowace ml (Standard bayani A).Tsarma sassan daidaitaccen bayani A ƙididdigewa tare da methanol don samun daidaitaccen maganin B mai ɗauke da 100 µg kowace ml da daidaitaccen bayani C mai ɗauke da 50 µg kowace ml.Aiwatar da ɓangarorin 20-µL na maganin gwajin da daidaitattun mafita B da C zuwa farantin chromatographic na bakin ciki mai dacewa (duba Chromatography 621), mai rufi da 0.25-mm Layer na chromatographic silica gel cakuda.Ƙirƙirar chromatogram a cikin tsarin narkewa wanda ya ƙunshi cakuda chloroform, methanol, da glacial acetic acid (79:14:7) har sai da gaban sauran ƙarfi ya motsa kusan kashi uku cikin huɗu na tsawon farantin.Cire farantin daga ɗakin, bushe-bushe, kuma bincika ƙarƙashin hasken UV mai ɗan gajeren zango: duk wani tabo ban da babban tabo da aka samu daga maganin gwajin bai wuce girman ko ƙarfin babban wurin da aka samu daga daidaitaccen bayani na B (1% ), da jimlar ƙazantattun abubuwan da aka wakilta ta duk wuraren ban da babban tabo, dangane da kwatanta ƙarfin irin waɗannan wuraren tare da ƙarfin manyan wuraren da aka samu daga Standard Solution B da C, bai wuce 2% ba. .
Assay-
Wayar hannu lokaci-Shirya dace tace cakuda ruwa, methanol, da glacial acetic acid (55:45:0.1).Yi gyare-gyare idan ya cancanta (duba Dacewar Tsarin ƙarƙashin Chromatography 621).
Daidaitaccen shiri- Narkar da adadin USP Chloramphenicol RS daidai gwargwado a cikin lokacin Wayar hannu, kuma a tsarma ƙididdigewa, da mataki-mataki idan ya cancanta, tare da lokacin Wayar hannu don samun mafita yana da sananniya na kusan 80 µg kowace ml.Tace wani yanki na wannan maganin ta hanyar tacewa na 0.5-µm ko mafi kyawun porosity filter, kuma amfani da tsararren tacewa azaman daidaitaccen shiri.
Shirye-shiryen Assay- Canja wurin kusan 200 MG na Chloramphenicol, daidai a auna, zuwa faifan volumetric 100-mL, ƙara lokacin Wayar hannu zuwa ƙara, da haɗuwa.Canja wurin 4.0 ml na maganin da aka samu zuwa 100-mL flask volumetric, tsarma tare da Wayar hannu zuwa girma, da haɗuwa.Tace wani yanki na wannan maganin ta hanyar tacewa na 0.5-µm ko mafi kyawun porosity, kuma yi amfani da tsararren tacewa azaman shirin Assay.
Tsarin Chromatographic (duba Chromatography <621>) -Chromatograph na ruwa yana sanye da na'urar ganowa 280-nm da ginshiƙin 4.6-mm × 10-cm wanda ya ƙunshi 5-µm tattara L1.Matsakaicin kwarara shine kusan 1 ml a minti daya.Chromatograph the Standard shiri, da rikodin kololuwar martani kamar yadda aka umurce a ƙarƙashin Tsari: ingancin ginshiƙi da aka ƙaddara daga kololuwar nazari bai wuce faranti na ka'idar 1800 ba, ma'aunin wutsiya bai wuce 2.0 ba, kuma madaidaicin madaidaicin ƙa'idar don kwafin allurar ba ta kasance ba. fiye da 1.0%.
Tsari-[bayanin kula-Yi amfani da tsayin tsayi inda aka nuna kololuwar martani.] dabam dabam (kimanin 10 µL) na daidaitaccen shiri da shirye-shiryen Assay a cikin chromatograph, yi rikodin chromatograms, da auna martani ga manyan kololuwa.Yi ƙididdige adadin, a cikin MG, na C11H12Cl2N2O5 a cikin ɓangaren Chloramphenicol da aka ɗauka ta dabara:
2.5C (rU / rS)
a cikin abin da C shine maida hankali, a cikin µg a kowace ml, na USP Chloramphenicol RS a cikin Shirye-shiryen Daidaitawa, kuma rU da rS sune mafi girman martanin da aka samu daga shirye-shiryen Assay da kuma daidaitaccen shiri, bi da bi.