D-Leucine CAS 328-38-1 HD-Leu-OH Assay 98.5 ~ 101.5% Babban inganci

pH
Ɗauki 0.50g na wannan samfurin, ƙara 50ml na ruwa, zafi don narkewa, sanyi, kuma ƙayyade bisa ga doka (General 0631), ƙimar pH ya zama 5.5 ~ 6.5.
Isar da Magani
Ɗauki 0.50g na wannan samfurin, ƙara 50ml na ruwa, zafi don narke, sanyi, ultraviolet-bayanin gani spectrophotometry (General law 0401), auna watsawa a 430nm zangon, ba kasa da 98.0%.
Chloride
Ɗauki 0.25g na wannan samfurin kuma duba shi bisa ga doka (Gaba ɗaya doka 0801).Idan aka kwatanta da maganin kulawa da aka yi da 5.0 ml na daidaitaccen maganin sodium chloride, bai kamata ya kasance mai da hankali ba (0.02%).
Sulfate
Ɗauki 1.0g na wannan samfurin kuma duba shi bisa ga doka (Gaba ɗaya doka 0802).Idan aka kwatanta da maganin kulawa da aka yi da 2.0 ml na daidaitaccen bayani na potassium sulfate, bai kamata ya kasance mai girma ba (0.02%).
Ammonium gishiri
Ɗauki wannan samfurin 0.10g, duba bisa ga doka (Gabaɗaya doka 0808), kuma kwatanta tare da maganin sarrafawa da aka yi da daidaitaccen maganin ammonium chloride 2.0 ML, ba zurfi (0.02%).
sauran amino acid
Ɗauki adadin da ya dace na wannan samfurin, ƙara ruwa don narkar da kuma tsarma don yin maganin da ke dauke da kimanin 20mg a kowace lml a matsayin maganin gwaji;Ɗauki 1ml don ma'auni daidai kuma saka shi a cikin gilashin ma'auni na 200ml, tsoma ma'auni da ruwa, girgiza, a matsayin maganin sarrafawa;Ɗauki adadin da ya dace na leucine reference da valine reference, sa'an nan kuma saka shi a cikin gwargwado na aunawa, da ruwa da aka narkar da kuma diluted don shirya wani bayani dauke da game da 0.4mg kowane da 1ml a matsayin tsarin da ya dace bayani.Dangane da gwajin chromatography na bakin ciki (General 0502), sha mafita guda uku na sama kowane 5 u1, bi da bi, akan silica gel G bakin bakin ciki farantin, tare da n-butanol-water-glacial acetic acid (3:1:1). don haɓakawa, bayan ƙaddamarwa, bushewar iska, fesa tare da ninhydrin a cikin maganin acetone (1-50), dumama a 80 ° C har sai spots sun bayyana, da kuma bincika nan da nan.Maganin sarrafawa ya kamata ya nuna tabo mai haske, kuma tsarin da ya dace ya kamata ya nuna tabo guda biyu gaba ɗaya.Idan maganin gwajin ya nuna alamun rashin tsabta, launi bai kamata ya kasance mai zurfi (0.5%) fiye da babban wurin maganin sarrafawa ba.
Asara akan bushewa
Ɗauki wannan samfurin, bushe a 105 ℃ na tsawon sa'o'i 3, asarar nauyi ba zai wuce 0.2% ba (Dokar Janar 0831).
Ragowa akan Ignition
Ɗauki 1.0g na wannan samfurin kuma duba shi bisa ga doka (Gaba ɗaya doka 0841).Ragowar hagu kada ta wuce 0.1%.
Iron Gishiri
Ɗauki 1.5g na wannan samfurin kuma duba shi bisa ga doka (Gaba ɗaya doka 0807).Idan aka kwatanta da maganin kulawa da aka yi da 0.001% na daidaitaccen maganin ƙarfe, ba zai zama mai zurfi ba ().
Karfe masu nauyi
Ragowar da aka bari a ƙarƙashin abin shan ragowar ƙonewa ba zai ƙunshi fiye da sassa 10 a kowace miliyan na ƙarfe mai nauyi ba lokacin da doka ta bincika (General Principles 0821, Law II).
Gishiri arsenic
Add 5ml ruwa, 1ml sulfuric acid da 10ml sulfurous acid, zafi zuwa girma na game da 2ml a cikin wani ruwa wanka, ƙara 5ml ruwa, ƙara Dropwise ammonia bayani har sai mai nuna phenolphthalein ne tsaka tsaki, ƙara hydrochloric acid 5ml, ƙara ruwa don yin 28ml , bisa ga binciken doka (General Principles 0822 Dokar farko), ya kamata ya bi tanadin (0.0001%).
Bacterial Endotoxin
Ɗauki wannan samfurin, duba bisa ga doka (General 1143), kowane lg leucine mai dauke da endotoxin ya kamata ya zama ƙasa da 25EU.(Don allura)
328-38-1 - Ƙaddamar Abun ciki
Ɗauki wannan samfurin game da 0.1g, ma'auni madaidaici, ƙara anhydrous formic acid 1 ml narkar da, ƙara glacial acetic acid 25ml, bisa ga potentiometric titration Hanyar (General 0701), tare da perchloric acid titration bayani (O. 1 mol/L) titration. , kuma an gyara sakamakon titration tare da gwaji mara kyau.Kowane 1 ml na maganin titration perchloric acid (0.1 mol/L) yayi daidai da 13.12 mg na C6H13N02.