Fmoc-Gly-OH CAS 29022-11-5 Fmoc-Glycine Tsarkake> 99.0% (HPLC) Factory

Fmoc-Glycine (Fmoc-Gly-OH) (CAS: 29022-11-5) sigar Glycine ce mai kariya ta N-Fmoc.Glycine shine amino acid mara mahimmanci.Fmoc-amino acid, wanda aka yi amfani da shi a cikin kira na peptide, ana amfani dashi azaman tsaka-tsakin haɗin gwiwar kwayoyin halitta, matsakaicin magunguna, reagent biochemical ko reagent sinadarai.
Gabatarwa: Fmoc-Glycine Glycine shine kariya ta Fmoc.Fluorene methoxycarbonyl (Fmoc) wata ƙungiya ce mai mahimmanci mai mahimmanci, wanda za'a iya amfani dashi a cikin ruwa mai ammonia ko dioxane-methanol-4N NaOH (30: 9: 1) da piperidine, ethanolamine, cyclohexylamine, 1,4-Dioxane, pyrrolidone da sauran ammonia 50% dichloromethane bayani.A ƙarƙashin raunin alkaline kamar sodium carbonate ko sodium bicarbonate, Fmoc-Cl ko Fmoc-OSu ana gabatar da ƙungiyar kare Fmoc gabaɗaya.
Aikace-aikacen: Fmoc-Glycine shine Glycine mai kariya ta Fmoc, wanda galibi ana amfani dashi don haɗa polypeptides.Bambance-bambancen kungiyoyin kare amino acid yana da babban tasiri akan inganci da yawan amfanin haɗin gwiwar polypeptide.Misali, ana iya amfani da Fmoc-Glycine don shirya mai hana lalatawar glycine, kuma rukunin Fmoc-kariya da sarkar dogon hydrophobic suna da alaƙa da kwayoyin Glycine ta hanyar haɓakar amidation, da heteroatom (N, O, da sauransu) Ko kuma. unsaturated bonds) da zoben bisphenyl mai dauke da π electrons ana sanya su a kan karfe, kuma dogon sarkar hydrophobic yana toshe kwayoyin ruwa, ta yadda ya samar da fim mai kariya a saman karfe don cimma manufar kariya ta lalata.
Shiri: Fmoc-Glycine kira takamaiman matakai sune kamar haka: 3.0g Glycine an narkar da shi a cikin 115ml na 10% Na2CO3, sanyaya zuwa -4 ~ 0℃, 11.2g Fmoc-Cl narkar da 35.0ml na acetone kuma ƙara zuwa tsarin dropwise, Bayan juye juye, motsawa a cikin wanka na kankara na minti 30, sa'an nan kuma motsawa a dakin da zafin jiki na 2.0h.An kula da ci gaban da aka samu ta hanyar chromatography na bakin ciki.Bayan an gama maganin, an zuba 400ml na ruwa a cikin ruwa, an fitar da ether sau biyu, a sanyaya kuma an daidaita shi zuwa pH2 tare da hydrochloric acid mai mahimmanci, babban adadin farin da aka samo, an fitar da 50 ml na ethyl acetate sau uku, anhydrous magnesium. Sulfate ya bushe, an zubar da sauran ƙarfi a ƙarƙashin raguwar matsa lamba, kuma an yi amfani da ether na man fetur don hazo don samun 11.5g na Fmoc-Glycine.Bayan tsarkakewa, tsarkin samfurin ya kasance 97.35% ta babban aikin chromatography na ruwa.