L-4-Nitrophenylalanine Methyl Ester HCl CAS 17193-40-7 H-Phe(4-NO2) -OMe·HCl Assay>98.5% (HPLC) Factory

Hanyar Nazarin L-4-Nitrophenylalanine Methyl Ester HCl (H-Phe(4-NO2) -OMe·HCl (CAS: 17193-40-7)
Bayani: Sanya game da 2 g na samfurin a cikin tsabta da bushe petri-tasa kuma lura da bayyanar samfurin.Rahoto: Rahoto abin lura
Identificaiton: Kwatanta lokacin riƙewa na babban kololuwa a cikin chromatogram na shirye-shiryen gwaji tare da riƙe lokacin babban kololuwa a cikin chromatogram na dacewa da tsarin tsarin kamar yadda aka samu a cikin tsabtar chromatographic ta HPLC.
Asara akan bushewa: Busasshen gilashin da aka tsayar, kwalban auna mara zurfi a 105± 2℃ na mintuna 30.Bada shi yayi sanyi zuwa dakin da zafin jiki a cikin injin tsinke.Yi awo daidai gwargwado da ma'auni kuma yi rikodin nauyin (W1).Canja wurin kusan 1 zuwa 2g daidai gwargwado adadin kayan gwaji cikin kwalbar auna.Ta hanyar a hankali, girgiza gefe, rarraba samfurin gwajin daidai da yadda za'a iya aiwatarwa zuwa zurfin kusan mm 5.Auna kwalban awo da aka ɗora kuma a yi rikodin nauyi (W2).Sanya kwalban da aka ɗora a cikin tanda mai bushewa, cire madaidaicin kuma bar shi a cikin ɗakin.Bushe samfurin gwajin a 105± 2℃ na 3 hours.Bayan buɗe ɗakin, rufe kwalbar da sauri, kuma ba shi damar zuwa yanayin zafi a cikin injin na'ura.Yi auna daidai kwalbar aunawa tare da matsewa kuma sake maimaita nauyin (W3).Yi lissafin asarar kashi akan bushewar samfurin ta amfani da dabara mai zuwa:
Asarar ƙididdiga akan bushewa (% w/w) = (W2-W3) ÷ (W2-W1) x 100
Abubuwan Enantiomer na HPLC:
Chemical & Reagent:
n-Hexane AR darajar
Babban darajar HPLC
Isopropyl Alcohol HPLC darajar
Diethylamine AR darajar
Matsayi: L-4-Nitrophenylalanine Methyl Ester HCl misali;L-4-Nitrophenylalanine Methyl Ester HCl enantiomer misali
Wayar Hannu: Shirya cakuda mai kama da n-Hexane, Alcohol isopropyl, methanol da Diethylamine (65:20:15:0.1 v/v/v/v)
Shirye-shiryen Blank: Canja wurin 2.5ml na methanol a cikin faifan volumetric 10-ml, tsarma zuwa ƙara tare da lokacin wayar hannu da haɗuwa.
Shirye-shiryen dacewa da tsarin:
Yi auna daidai kuma canja wurin kusan 5 MG kowane L-4-Nitrophenylalanine Methyl Ester HCl daidaitaccen da L-4-Nitrophenylalanine Methyl Ester HCl enantiomer daidaitattun a cikin 10-ml volumetric flask, narke a cikin kusan 2.5ml na methanol, tsarma zuwa girma tare da wayar hannu. lokaci da Mix.
Shirye-shiryen gwaji:
Yi awo daidai kuma canja wurin kusan MG 5 na samfurin a cikin filastar volumetric 10-mol, narke a cikin kusan 2.5ml na methanol, tsarma zuwa ƙara tare da lokacin wayar hannu da haɗuwa.
Sigar kayan aiki:
Kayan aiki: HPLC sanye take da injector, Pump, UV detector da Recorder
Tushen: ChiralPak-IC (4.6mm x 250mm) 5 μ ko daidai
Yawan gudu: 1.0 ml / minti
Tsawon tsayi: 260nm
Girman allura: 10 μL
Yanayin zafi na ginshiƙi: 25 ℃
Lokacin gudu: minti 20
Tsari
Allurar mafita kamar yadda aka ambata a cikin jeri mai zuwa.Yi rikodin chromatograms kuma tabbatar da ma'auni:
Bayanin Alurar Yawan Ma'auni na allura
Shirye-shiryen Blank 1 Babu kololuwar tsoma baki da yakamata a lura a lokacin riƙewar L-4-Nitrophenylalanine Methyl Ester HCl ko enantiomer.
Shirye-shiryen dacewa da tsarin 1 ƙuduri tsakanin L-4-Nitrophenylalanine Methyl Ester HCl da kololuwar enantiomer bai kamata ya zama ƙasa da 2.0 ba.
Shirye-shiryen gwaji 1 Yi amfani da chromatogram gwaji don tantance abun ciki na enantiomer a cikin samfurin.
Lokutan Tsayawa na Dangi:
L-4-Nitrophenylalanine Methyl Ester HCl 1.0
Enantiomer Game da 1.11
Lokacin riƙe L-4-Nitrophenylalanine Methyl Ester HCl kusan mintuna 9.8 ne.
Lissafi: A cikin chromatogram da aka samu daga shirye-shiryen gwaji, yin watsi da duk kololuwar tsammanin L-4-Nitrophenylalanine Methyl Ester HCl da Enantiomer.Bayar da rahoton abun ciki na enantiomer a cikin samfurin ta hanyar daidaita yanki.
Chromatogrpahic Tsarkakewa da Tsabtace ta HPLC:
Chemical & Reagent:
Diammonium phosphate AR darajar
Phosphoric acid AR darajar
Babban darajar HPLC
Ruwan Tsarkakewa
Matsayi
L-4-Nitrophenylalanine Methyl Ester HCl misali
4-Nitro-L-Phenylalanine monohydrate misali
Wayar hannu Phase-A
Narke 6.6 g na Diammonium phosphate a cikin 1000ml na ruwa mai tsabta.Daidaita pH na wannan maganin zuwa 6.0 tare da Phosphoric acid.Tace da dagaske.Kafin auna pH na bayani, daidaita ma'aunin pH tare da daidaitaccen bayani mai buffer pH 4.01 AND 7.00.
Wayar hannu Phase-B
Babban darajar HPLC
Tsarin Dacewar Tsarin
Yi nauyi daidai kuma canja wurin kusan 25 MG kowane L-4-Nitrophenylalanine Methyl Ester HCl daidaitaccen da daidaitaccen 4-Nitro-L-Phenylalanine monohydrate a cikin faifan volumetric 50-ml, narkar da cikin kuma tsarma zuwa girma tare da methanol da haɗuwa.
Shirye-shiryen Gwaji:
Yi auna daidai kuma canja wurin samfurin kusan 25 MG a cikin faifan volumetric 50-ml, narke a ciki kuma a tsoma zuwa ƙara tare da methanol da haɗuwa.
Sigar kayan aiki:
Kayan aiki: HPLC sanye take da injector, Pump, UV detector da Recorder
Rukunin: XTerra RP-18 (4.6mm x 150mm) 5 μ ko daidai
Yawan gudu: 1.0 ml / minti
Tsawon tsayi: 265nm
Girman allura: 20 μL
Yanayin zafi na ginshiƙi: 25 ℃
Lokacin gudu: minti 45
Shirin Gradient
Lokaci (min) Matakin Waya-A % Waya Phase-B %
0.0190 10
15 60 40
35 40 60
40 35 65
4190 10
45 90 10
Tsari:
Allurar mafita kamar yadda aka ambata a cikin jeri mai zuwa.Yi rikodin chromatograms kuma tabbatar da ma'auni:
Shirye-shiryen Blank 1 Babu kololuwar tsangwama da yakamata a lura a lokacin riƙewar L-4-Nitrophenylalanine Methyl Ester HCl.
Shirye-shiryen dacewa da tsarin 1 Tailing factor don L-4-Nitrophenylalanine Methyl Ester HCl kololuwar bai kamata ya zama ƙasa da 2.0 ba.
Shirye-shiryen gwaji 1 Yi amfani da chromatogram gwaji don tantance tsafta da ƙazanta na chromatographic.
Lokutan Tsayawa na Dangi:
L-4-Nitrophenylalanine Methyl Ester HCl 1.0 (lokacin riƙewa kusan mintuna 11.4)
4-Nitro-L-Phenylalanine Game da 0.4
Toluene Game da 2.3
Lissafi:
A cikin chromatogram da aka samo daga shirye-shiryen gwaji, bayan gyaran gyare-gyare, watsi da kololuwar saboda toluene.Ba da rahoton tsabtar chromatographic, abun ciki na 4-Nitro-L-Phenylalanine da jimlar ƙazanta a cikin samfurin ta hanyar daidaita yanki.