L-Cysteine ​​CAS 52-90-4 (H-Cys-OH) Assay 98.5 ~ 101.0% (Titration) Babban Ingancin Factory

L-Cysteine ​​​​ya ƙunshi ƙasa da 98.5% kuma bai wuce 101.0% na L-Cysteine ​​​​(C3H7NO2S), wanda aka ƙididdige shi akan tushen busasshen.
Bayanin L-Cysteine ​​​​yana faruwa a matsayin farin lu'ulu'u ko farin lu'ulu'u.Yana da ƙamshi mai siffa da ɗanɗano mai daɗi.Yana iya narkewa cikin ruwa, kuma a zahiri baya narkewa a cikin ethanol (99.5).Yana narkewa a cikin 1 mol/L hydrochloric acid TS.
Identification Ƙayyade nau'in shayarwar infrared na L-Cysteine ​​​​kamar yadda aka umarce shi a cikin hanyar faifan potassium bromide a ƙarƙashin Infrared Spectrophotometry <2.25>, kuma kwatanta bakan tare da Spectrum Reference: duka bakan suna nuna irin ƙarfin sha a lambobi iri ɗaya.
Juyawa na gani <2.49> [a] 20D: + 8.0 ~ + 10.0 ° (2 g da aka lissafta akan busasshiyar tushe, 1 mol/L hydrochloric acid TS, 25mL, 100 mm).
pH <2.54> pH na maganin da aka shirya ta hanyar narkar da 1.25 g na L-Cysteine ​​​​a cikin 50 ml na ruwa shine 4.7 zuwa 5.7.
Tsaftace (1) Tsaftace da launi na bayani-Narke 1.0 g na L-Cysteine ​​​​a cikin 20 ml na ruwa: bayani a bayyane yake kuma mara launi.
(2) Chloride <1.03>-Narke 0.30 g na L-Cysteine ​​​​a cikin 10mL na nitric acid diluted (1 a cikin 4), ƙara 10mL na hydrogenperoxide (30), zafi minti 20 a cikin ruwan zãfi, wanka, sannan ƙara. ruwa don yin 50 ml.Yi gwajin ta amfani da wannan maganin azaman maganin gwajin.Shirya maganin sarrafawa tare da 0.35 ml na 0.01 mol/L hydrochloric acid VS (ba fiye da 0.041%)
(3) Sulfate <1.14>-Narke 0.6 g na L-Cysteine ​​​​a cikin 30 ml na ruwa da 3 ml na dilute hydrochloric acid, kuma ƙara ruwa don yin 50 ml.Yi gwajin ta amfani da wannan maganin azaman maganin gwajin.Shirya maganin kulawa kamar haka: Zuwa 0.35 ml na 0.005 mol/L sulfuric acid VS ƙara 3 ml na dilute hydrochloric acid da ruwa don yin 50 ml.Shirya maganin gwaji da maganin kulawa tare da 4 ml na barium chloride TS, bi da bi (ba fiye da 0.028%) ba.
(4) Ammonium <1.02> -Yi gwajin tare da 0.25 g na L-Cysteine ​​​​, ta yin amfani da distillation a ƙarƙashin rage matsa lamba.Shirya maganin sarrafawa tare da 5.0 ml na Standard Ammonium Solution (ba fiye da 0.02%) ba.
(5) Ƙarfe mai nauyi <1.07> - Ci gaba tare da 1.0g na L-Cysteine ​​​​ bisa ga Hanyar 4, da kuma yin gwajin. Shirya maganin sarrafawa tare da 1.0 mL na Daidaitaccen Maganin Gubar (ba fiye da 10 ppm ba).
(6) Iron <1.10> -Shirya maganin gwajin gwajin tare da 1.0 g na L-Cysteine ​​​​bisa ga Hanyar 1, kuma kuyi gwajin bisa ga Hanyar A. Shirya maganin sarrafawa tare da 1.0mL na Standard Iron Solution (ba fiye da 10 ppm ba. ).
(7) Abubuwan da suka danganci-Narke 0.10 g na L-Cysteine ​​​​a cikin maganin N-ethylmaleimide (1 a cikin 50) don yin daidai 10 ml, bar minti 30, kuma amfani da wannan bayani azaman samfurin samfurin.Pipet 1 ml na maganin samfurin, ƙara ruwa don yin daidai 10 ml, pipet 1 ml na wannan maganin, ƙara ruwa don yin daidai 50 ml, kuma amfani da wannan maganin azaman daidaitaccen bayani (1).Na dabam, narke 0.10 g na L-cystine a cikin 0.5mol/L hydrochloric acid TS don yin daidai 20 ml.Pipet 1ml na wannan maganin, ƙara ruwa don yin 100 ml, kuma amfani da wannan maganin azaman daidaitaccen bayani (2).Yi gwajin tare da waɗannan mafita kamar yadda aka umurce su a ƙarƙashin Chromatography na Thin-Layer<2.03>.Spot 10mL kowane samfurin bayani da daidaitattun mafita (1) da (2) akan farantin gel na silica don chromatography na bakin ciki.Ci gaba da farantin tare da cakuda 1-butanol, ruwa, da acetic acid (100) (3: 1: 1) zuwa nisa na kimanin 10 cm, kuma bushe farantin don minti 30 a 809C. Fesa farantin daidai da bayani. na ninhydrin a cikin cakuda methanol da acetic acid (100) (97: 3) (1 a cikin 100), sa'an nan kuma zafi a 80 ℃ na minti 10: wurin da aka samo daga samfurin bayani daidai da tabo da aka samu daga daidaitaccen bayani. (2) bai fi tabo ba daga madaidaicin bayani (2).Har ila yau, wuraren da ban da babban tabo da wuraren da aka ambata a sama daga maganin samfurin ba su da tsanani fiye da tabo daga daidaitaccen bayani (1).
Asara akan bushewa <2.41> Ba fiye da 0.50% (1 g, a cikin injin, phosphorus (V) oxide, 3 hours).
Ragowa akan ƙonewa <2.44> Bai wuce 0.1% ba (1 g).
Auna nauyi daidai 0.2 g na L-Cysteine ​​​​, sanya shi a cikin kwandon tsayawa, sa'annan a narke cikin 20 ml na ruwa.Narke 4 g na potassium iodide a cikin wannan bayani, nan da nan sanya a cikin ruwan sanyi kankara, ƙara 5 ml na dilute hydrochloric acid da daidai 25 ml na 0.05 mol/L iodine VS, bar a cikin duhu wuri na minti 20, sa'an nan titrate <2.50 Mafi yawan adadin iodine tare da 0.1 mol/L sodium thiosulfate VS (mai nuna alama: sitaci TS).Yi ƙayyadaddun ƙaddara ta amfani da hanya ɗaya.
Kowane ml na 0.05 mol/L iodine VS 12.12 MG na C3H7NO2S
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