L-Cystine CAS 56-89-3 (H-Cys-OH) 2 Assay 98.5 ~ 101.0% (Titration) Babban Ingancin Factory

BAYANI
Cystine ya ƙunshi NLT 98.5% da NMT 101.5% na C6H12N2O4S2, kamar yadda L-Cystine, ƙididdiga akan busasshen tushe.
GANO
A. RASHIN NUTSUWA <197K>
B. JIGAWA NA gani, Jujjuya Takamaiman <781S>: -215 zuwa -225, an ƙaddara a 20°
Maganin samfurin: 20 mg/mL, a cikin 1 N hydrochloric acid
C. Ƙimar RF na babban tabo na Samfurin Magani a cikin gwaji don Najasa Tsarin Halitta ya yi daidai da na daidaitaccen bayani.
ASSAY
TSARI
Maganin samfurin: Canja wurin kimanin 0.1 g na Cystine zuwa gilashin da aka dakatar da gilashi, kuma a narke a cikin cakuda 2 ml na dilute sodium hydroxide (1 a cikin 20) da 10 ml na ruwa.Ƙara 10 ml na potassium bromide bayani (200 g/L a cikin ruwa), 50.0 ml na 0.1 N potassium bromate VS, da 15 ml na dilute hydrochloric acid (17 a 100).
Nan da nan saka abin tsayawa a cikin flask, kuma a kwantar da shi a cikin ruwan wanka na kankara.Bada damar tsayawa kariya daga haske na minti 10.
Titrimetric tsarin
(Duba Titrimetry <541>)
Yanayin: Ragowar titration
Titrant: 0.1 N potassium bromate VS
Baya-titrant: 0.1 N sodium thiosulfate VS
Gano ƙarshen ƙarshen: Launimetric
Daidaita: Kowane ml na 0.1 N potassium bromate VS daidai yake da 2.403 MG na C6H12N2O4S2 akan busasshiyar tushe.
Analysis: Ƙara 1.5 g na potassium iodide, kuma bayan 1minti, titrate tare da 0.1 N sodium thiosulfate VS, ta amfani da sitaci TS a matsayin mai nuna alama.Yi ƙudiri mara kyau, kuma a yi duk wani gyara da ya dace.
Sharuɗɗan karɓa: 98.5% ~ 101.5% akan busasshiyar tushe
RASHIN ZUCIYA
Najasa Na Inorganic
SAURAN WUTA <281>: NMT 0.1%
CLORIDE DA SULFATE, Chloride <221>: NMT 200 ppm.Wani yanki na 0.7-g yana nuna babu ƙarin chloride fiye da daidai da 0.40 ml na 0.01 N hydrochloric acid.
CHLORIDE DA SULFATE, Sulfate <221> NMT 200 ppm.Wani yanki na 1.2-g yana nuna babu sauran sulfate fiye da daidai da 0.25 ml na 0.020 N sulfuric acid.
IRON <241>: NMT 10ppm
KARFE MAI KYAU, Hanyar I <231>: NMT 10ppm
Najasa Na Halitta
TSARI
Daidaitaccen bayani: Narkar da adadin USP Cystin RS a cikin 1 N hydrochloric acid, kuma a tsoma shi da ruwa don samun bayani yana da sananniya na kusan 0.02 mg/mL.
Maganin samfurin: Narkar da adadin Cystine a cikin 1 N hydrochloric acid, kuma a tsoma shi da ruwa don samun bayani yana da sananniya na kusan 10 mg/mL.
Maganin dacewa da tsarin: Narkar da adadin USP Cytine RS da USP Arginine Hydrochloride RS a cikin 1 N hydrochloric acid, kuma a tsomasu da ruwa don samun bayani yana da sananniya na kusan 0.4 mg/mL kowanne.
Tsarin Chromatographic (Duba Chromatography <621>, Chromatography na bakin-Layer.)
Yanayin: TLC
Adsorbent: 0.25-mm Layer na chromatographic silica gel cakuda
Girman aikace-aikacen: 5μL
Haɓaka tsarin narkewa: cakuda ammonia da 2-propanol (3: 7)
Fesa reagent: Narke 0.2 g na ninhydrin a cikin 100 ml na cakuda butanol da 2 N acetic acid (95:5).
Bincike
Samfura: Daidaitaccen Magani, Maganin Samfurin, da Maganin dacewa da tsarin
Ci gaba kamar yadda aka ba da umarni don Chromatography <621>, Chromatography na Baƙaƙe.Bayan shan iska farantin, fesa da Fesa reagent, kuma zafi tsakanin 100 ° da 105 ° na 15 min.Yi nazarin farantin.Na'urar chromatogram daga tsarin dacewa da tsarin yana nuna aibobi biyu a sarari.
Sharuɗɗan karɓa: Duk wani wuri na biyu daga Maganin Samfurin bai fi girma ko mafi tsanani fiye da ainihin tabo na daidaitaccen bayani ba.
Najasa ɗaya: NMT 0.2%
Jimlar ƙazanta: NMT 2.0%
MAKAMMAN JARRABAWA
RASHIN BUSHEWA <731>: bushe samfurin a 105 ℃ na 3 h: yana rasa NMT 0.2% na nauyinsa.
KARIN BUKATA
KYAUTA DA ARJIYA: Ajiye a cikin kwantena masu kyau, kuma adana a yanayin zafin ɗaki mai sarrafawa.
MATSAYIN NAZARI NA USP <11>
USP Arginine Hydrochloride RS
USP Cystine RS