L-Glutamine CAS 56-85-9 (H-Gln-OH) Assay 99.0 ~ 101.0% Babban Ingancin Masana'antu

Japan PharmacopoeiaKa'idojin Magana
L-Glutamine (56-85-9)
L-Glutamine, lokacin da aka bushe, ya ƙunshi bai wuce 99.0% ba kuma bai wuce 101.0% na L-Glutamine (C5H10N2O3).
Bayanin L-Glutamine yana faruwa azaman fari, lu'ulu'u ko foda.Yana da ɗanɗano ɗanɗanon siffa
Yana narkewa da yardar kaina a cikin formic acid, mai narkewa cikin ruwa, kuma a zahiri baya narkewa a cikin ethanol (99.5)
Identification Ƙayyade nau'in shayarwar infrared na L-Glutamine kamar yadda aka umarce shi a cikin hanyar faifan potassium bromide a ƙarƙashin Infrared Spectrophotometry <2.25>, kuma kwatanta bakan tare da Bakin Magana: duka bakan suna nuna irin ƙarfin sha a lambobi iri ɗaya.
Juyawa na gani <2.49> [a] 20D: + 6.3 ° zuwa + 7.3° Yi nauyi daidai game da 2 g na L-Glutamine, a baya an bushe, ƙara 45mL na ruwa, dumi zuwa 40 ℃ don narkewa, kuma bayan sanyaya, ƙara ruwa don yin daidai 50 ml.Ƙayyade jujjuyawar gani na wannan bayani a cikin tantanin halitta 100-mm, cikin mintuna 60.
pH <2.54> pH na maganin da aka shirya ta hanyar narkar da 1.0 g na L-Glutamine a cikin 50 ml na ruwa yana tsakanin 4.5 da 6.0.
Tsafta
(1) Tsare-tsare da launi na bayani - Maganin da aka samu ta hanyar narkar da 0.5 g na L-Glutamine a cikin 20 ml na ruwa ya bayyana kuma mara launi.
(2) Chloride <1.03> -Yi gwajin tare da 0.5 g na L-Glutamine.Shirya maganin sarrafawa tare da 0.30 ml na 0.01 mol / L hydrochloric acid VS (ba fiye da 0.021%) ba.
(3) Sulfate <1.14> - Yi gwajin tare da 0.6 g na L-Glutamine.Shirya maganin sarrafawa tare da 0.35 ml na 0.005 mol/L sulfuric acid VS (ba fiye da 0.028z ba).
(4) Ammonium <1.02> - Yi gwajin tare da 0.10 g na L-Glutamine, ta yin amfani da distillation a ƙarƙashin rage matsa lamba.Shirya maganin sarrafawa tare da 10.0 ml na Standard Ammonium Solution.Zazzabi na wanka na ruwa shine 45 ℃ (bai wuce 0.1%) ba.
(5) Karfe masu nauyi <1.07> - Ci gaba da 1.0 g na L-Glutamine bisa ga Hanyar 1, kuma yi gwajin.Shirya maganin sarrafawa tare da 1.0 ml na Madaidaicin Maganin Gubar (bai wuce 10 ppm ba).
(6) Iron <1.10>-Shirya maganin gwajin gwaji tare da 1.0 g na L-Glutamine bisa ga Hanyar 1, kuma yi gwajin bisa ga Hanyar A. Shirya maganin sarrafawa tare da 1.0 ml na Standard Iron Solution (ba fiye da 10 ba. ppm)
(7) Abubuwan da ke da alaƙa-Narke 0.10 g na L-Glutaminein 10 ml na ruwa, kuma amfani da wannan bayani azaman maganin samfurin.Pipet 1 ml na maganin samfurin, ƙara ruwa don yin daidai 10 ml.Pipet 1 ml na wannan maganin, ƙara ruwa don yin daidai 50 ml, kuma amfani da wannan bayani azaman daidaitaccen bayani.Yi gwajin tare da waɗannan mafita kamar yadda aka umurce su ƙarƙashin Thin-Layer Chromatography <2.03>.Spot 5mL kowane samfurin bayani da daidaitaccen bayani akan farantin silicagel don chromatography na bakin ciki-Layer.Sa'an nan kuma ci gaba tare da haɗin 1-butanol, ruwa da acetic acid (100) (3: 1: 1) zuwa adistance na kimanin 10 cm, kuma bushe farantin a 80 ℃ na minti 30.Fesa maganin ninhydrin daidai a cikin cakuda methanol da acetic acid (100) (97: 3) (1 cikin 100) akan farantin, kuma zafi a 80 ℃ na minti 10: wurin ban da babban tabo da aka samu tare da samfurin samfurin bai fi tsanani fiye da tabo da aka samu tare da daidaitaccen bayani ba.
Asarar bushewa <2.41> Bai wuce 0.3% ba (1 g, 105 ℃, awanni 3)
Ragowa akan ƙonewa <2.44> Bai wuce 0.10% ba (1 g).
Assay Yi nauyi daidai game da 0.15 g na L-Glutamine, wanda aka bushe a baya, narke cikin 3 ml na formic acid, ƙara 50 ml na acetic acid (100), da titrate <2.50> tare da 0.1 mol/L perchloric acid VS (titration potentiometric).Yi ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun tsari, da yin duk wani gyara da ya dace.
Kowane ml na 0.1 mol/L perchloric acid VS 14.61 MG na C5H10N2O3
Kwantena da Akwatunan Ajiye - Kwantena masu tsauri.

Ma'aunin Magana na USP <11>-USP Glutamine RS.
Ganewa, Ciwon Infrared <197K>.
Takamaiman Juyawa <781S>: tsakanin + 6.3 ° da + 7.3 °, ƙaddara a 20 ℃
Magani na Gwaji- A cikin flask mai dacewa, daidai shirya maganin kusan 40mg kowace ml a cikin ruwa a kusan 40 ℃.Cool da tsarma da ruwa zuwa ƙarar kafin amfani.
Asarar bushewa <731>- bushe shi a 105 ℃ na 3 hours;bai wuce 0.20% na nauyinsa ba.
Ragowa akan kunnawa <281>Ba fiye da 0.30%
Chloride <221>-Kashi na 0.7-g yana nuna babu chloride fiye da daidai da 0.50ml na 0.020 N hydrochloric acid: ba a sami fiye da 0.05% ba.
Sulfate <221>-A kashi 0.8-g ya nuna babu sulfate fiye da daidai da 0.25ml na 0.020 N sulfuric acid: ba a sami fiye da 0.03% ba.
Tsaftar Chromatographic
Adsorbent: 0.25-mm Layer na chromatographic silica gel cakuda.
Maganin Gwajin: 10mg a kowace ml, cikin ruwa
Daidaitaccen Magani-Shirya maganin USP Glutamine RS a cikin ruwa yana da sananniya na kusan 0.05mg a kowace ml.
Yawan aiki: 5μL
Haɓaka Tsarin Magani: cakuda barasa butyl, ruwa, da glacial acetic acid (3:1:1).
Fesa Reagent-Narke 0.2g na ninhydrin a cikin 100ml na cakuda butyl barasa da 2 N acetic acid (95:5)
Ci gaba kamar yadda aka umarce shi don chromatography na bakin ciki a ƙarƙashin Chromatography <621>, sannan a bushe farantin a 80 ℃ na mintuna 30.Fesa farantin tare da reagent na fesa, zafi a 80 ℃ na minti 10, kuma bincika ƙarƙashin farin haske: babu wani wuri na biyu a cikin chromatogram da aka samo daga maganin gwajin da ya fi girma ko mafi tsanani fiye da babban tabo a cikin chromatogram da aka samu daga mafita na standrad. (0.5%)
Najasa maras tabbas, Hanyar I <467>: Haɗu da buƙatun.
Asssy- Canja wurin game da 150mg na Glutamine, daidai nauyi, zuwa 125ml flask, narke a cikin 3ml na formic acid da 50ml na glacial acetic acid, da kuma titrate tare da 0.1 N perchloric acid VS kayyade karshen potentiometrically.Yi ƙayyadaddun ƙaƙƙarfan ƙaƙƙarfan ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun gyare-gyare (duba Titrimetry <541>).Kowane ml na 0.1 N perchloric acid yayi daidai da 14.615 MG na C5H10N2O3