L-Leucine Methyl Ester Hydrochloride CAS 7517-19-3

1. Bayyanar
Ɗauki adadin da ya dace na wannan samfurin, sanya shi a kan takarda mai laushi kuma yada 5 cm2 da 5 mm lokacin farin ciki.Duba shi a wuri mai haske.
2. Tsafta (HPLC)
2.1 Kayan aiki
Babban aikin chromatography ruwa, mai gano PDA.
Ma'aunin nazari na lantarki
2.2 Reagents
Acetonitrile (makin chromatographic), TFA (jin chromatographic)
2.3 Yanayi na chromatographic
2.3.1 Rukunin: YMC-ODS-AM, 5 μl, 150 x 4.6 mm
2.3.2
Tsawon Gano: INC220nm
Yawan gudu: 1.0ml/min
Girman allura: 10 μL (don tunani)
Diluent: acetonitrile
Lokacin gudu: minti 20
2.4 Shirye-shiryen lokaci na wayar hannu
Wayar hannu lokaci A (0.1% trifluoroacetic acid ruwa): daidai sha 2.0ml TFA, Tsarma zuwa 2000ml da ruwa, Mix da degas;
Wayar hannu lokaci B (0.1% trifluoroacetic acid acetonitrile): daidai sha 2.0ml TFA, Tsarma zuwa 2000ml tare da acetonitrile, Mix da degas;
2.5 Siffofin kayan aiki
Lokaci (min) A% B%
0.0090 10
12.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
2.6 Shiri na samfurin bayani
Yi nauyi da narkar da samfurin 0.1g tare da acetonitrile, tsarma zuwa 100ml, girgiza da kyau don amfani, ko taro iri ɗaya.Shirya samfurori guda biyu a layi daya.
2.7 Samfurin Ƙaddamarwa
Yi nazarin samfurin bisa ga tsarin samfur mai zuwa:
Fiye da allura 1 na bayani mara kyau
Maganin samfurin allura 1 1#
Maganin samfurin allura 1 2#
2.8 Kididdigar sakamako
2.8.1 An yi amfani da hanyar daidaita yanayin yanki mafi girma don ƙididdige tsaftar HPLC ta hanyar cire sarari mara kyau.
2.8.2 Ma'anar ma'anar ma'anar tsarkin allura biyu ba za ta fi 1% ba.
2.8.3 Idan sakamakon alluran biyu sun cika ka'idojin yarda, ana ɗaukar matsakaicin tsafta azaman sakamako na ƙarshe.
3, Matsayin narkewa -- RY-1 kayan aikin narkewa
4. Asarar hanyar gwajin bushewa
4.1 Kayan aiki:
Tanda mai bushewa mai zafi na lantarki, ma'auni 1/10,000.
4.2 Tsari:
A cikin kwalabe mai nauyi tare da madaidaicin nauyi da murfin bakin ƙasa mai bushewa, auna gram 1 (daidai zuwa gram 0.0001) na samfurin.Samfurin ya kamata a ko'ina yada a kasan kwalban auna tare da kauri na ba fiye da 10mm, sa a cikin wani thermostatic lantarki bushe tanda, bushe a 105 ~ 110 ℃ na 3 hours, sa'an nan kuma matsa zuwa cikin bushewa dakin don kwantar da hankali. zafin dakin don aunawa.
Lissafi: Asarar bushewa %= (M1-M2) ÷M×100
Inda: M1: nauyin samfurin da kwalban auna kafin bushewa, grams
M2: nauyin samfurin da kwalban aunawa bayan bushewa, a cikin grams
M: Nauyin samfurin, grams
5. Takamaiman juyawa
-- Ana auna takamaiman juyawa daidai da GB/T613-1988
Shirye-shiryen samfurin: 1.0000g samfurin an auna daidai kuma an motsa shi zuwa kwalban 50ml mai tsabta mai tsabta da bushe, an ƙara ruwa 20ml, kwalban an rufe shi da girgiza don narkewa, sa'an nan kuma an diluted zuwa sikelin da ruwa.
Gwaji: Daidaita sifilin gyroscope kafin gwajin, sannan ɗora bututun gwajin tare da maganin samfurin, yi rikodin kusurwar juyawa, kuma ƙididdige takamaiman juyawa na samfurin tare da dabara mai zuwa.
D20 = (r×50) ÷ (L×W)
[a] D20: Takamaiman juyawa na gani a 20 ℃ na maganin samfurin
r: Juyawa na gani da aka lura a 20 ℃ don maganin samfurin
50: Girman maganin samfurin da aka shirya (ml)
w: Misalin nauyi (g)
L: Tsawon bututun juyawa na gani (dm)