L-Phenylalanine CAS 63-91-2 (H-Phe-OH) Assay 98.5 ~ 101.5% Babban Ingancin Masana'antu

L-Phenylalanine, lokacin da aka bushe, ya ƙunshi ƙasa da 98.5% na C9H11NO2.
Bayanin L-Phenylalanine yana faruwa azaman farin lu'ulu'u ko foda crystalline.Ba shi da wari ko kuma yana da kamshin siffa, kuma yana da ɗanɗano mai ɗaci.Yana narkewa da yardar kaina a cikin formic acid, mai ɗanɗano kaɗan cikin ruwa, kuma a zahiri baya narkewa a cikin ethanol (95).Yana narkewa a cikin dilute hydrochloride acid.
Identification Ƙayyade nau'in shayarwar infrared na L-Phenylalanine, wanda aka bushe a baya, kamar yadda aka umarce shi a cikin hanyar faifan faifan potassium bromide a ƙarƙashin Infrared Spectrophotometry <2.25>, kuma kwatanta bakan tare da Spectrum Reference: duka bakan suna nuna irin ƙarfin sha a lambobi iri ɗaya. .
Juyawar gani <2.49> [a] D20: -33.0° zuwa -35.59° (bayan bushewa, 0.5 g, ruwa, 25 ml, 100 mm).
pH <2.54> Narke 0.20 g na L-Phenylalanine a cikin 20 ml na ruwa: pH na wannan maganin yana tsakanin 5.3 da 6.3
Tsafta (1) Tsaftace da launi na bayani-Narke 0.5 g na L-Phenylalanine a cikin 10 ml na 1 mol hydrochloric acid TS: bayani a bayyane yake kuma mara launi.
(2) Chloride <1.03> -Yi gwajin tare da 0.5 g na L-Phenylalanine.Shirya maganin sarrafawa tare da 0.30 ml na 0.01 mol / L hydrochloride acid VS (ba fiye da 0.021%) ba.
(3) Sulfate <1.14> - Yi gwajin tare da 0.6 g na L-Phenylalanine.Shirya maganin sarrafawa tare da 0.35 ml na 0.005 mol/L sulfuric acid VS (ba fiye da 0.028z ba).
(4) Ammonium <1.02> -Yi gwajin tare da 0.25 g na L-Phenylalanine.Shirya maganin sarrafawa tare da 5.0 ml na Standard Ammonium Solution (ba fiye da 0.02%) ba.
(5) Karfe masu nauyi <1.07>-Narke 1.0 g na L-Phenylalanine a cikin 40 ml na ruwa da 2 ml na dilute acetic acid ta dumama, sanyi, kuma ƙara ruwa don yin 50 ml.Yi gwajin ta amfani da wannan maganin azaman maganin gwajin.Shirya maganin kulawa kamar haka: zuwa 2.0 ml na Standard Lead Solution ƙara 2 ml na tsarma acetic acid da ruwa don yin 50 ml (ba fiye da 20 ppm ba).
(6) Arsenic <1.11> -Narke 1.0 g na L-Phenylalanine a cikin 5 ml na dilute hydrochloride acid da 15 ml na ruwa, da kuma yin gwajin tare da wannan bayani a matsayin maganin gwajin (ba fiye da 2 ppm ba).
(7) Abubuwan da ke da alaƙa-Narke 0.10 g na L-Phenylalanine a cikin 25 ml na ruwa, kuma amfani da wannan bayani azaman samfurin samfurin.Pipet 1 ml na maganin samfurin, kuma ƙara ruwa don yin daidai 50 ml.Pipet 5 ml na wannan maganin, ƙara ruwa don yin daidai 20 ml, kuma amfani da wannan maganin azaman daidaitaccen bayani.Yi gwajin tare da waɗannan mafita kamar yadda aka umurce su ƙarƙashin Thin-Layer Chromatography <2.03>.Spot 5mL kowane samfurin samfurin da daidaitaccen bayani akan farantin gel silica don chromatography na bakin ciki.Ci gaba da farantin tare da cakuda 1-butanol, ruwa da acetic acid (100) (3: 1: 1) zuwa adistance na kimanin 10 cm, kuma bushe farantin a 809Cfor30minutes.Fesa maganin ninhydrin a ko'ina a cikin acetone (1 in50) akan farantin, kuma zafi a 80 ℃ na mintuna 5: aibobi banda babban tabo daga maganin samfurin ba su da ƙarfi fiye da tabo daga daidaitaccen bayani.
Asara akan bushewa <2.41> Bai wuce 0.30% ba (1 g, 105 ℃, awanni 3).
Ragowa akan ƙonewa <2.44> Bai wuce 0.10% ba (1 g).
Assay Yi nauyi daidai game da 0.17 g na L-Phenylalanine, wanda aka bushe a baya, kuma a narke a cikin 3 ml na formic acid, ƙara 50mL na acetic acid (100), da titrate <2.50> tare da 0.1 mol/L perchloric acid VS (titration mai ƙarfi).Yi ƙudiri mara kyau, kuma a yi duk wani gyara da ya dace.
Kowane ml na 0.1 mol/L perchloric acid VS 16.52 MG na C9H11NO2
Kwantena da Akwatunan ajiya - Kwantena masu tsauri.