L-(-)-Tyrosine CAS 60-18-4 (H-Tyr-OH) Assay 98.5 ~ 101.5% Babban Ingancin Masana'anta

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) AJI 97 Hanyar Gwaji
Ganewa: Kwatanta nau'in infrared na samfurin tare da ma'auni ta hanyar fayafai bromide.
Takamaiman Juyawa [α] 20/D: Samfurin Busasshen, C=5, 1mol/L HCl
Halin Magani (Mai watsawa): 0.5g a cikin 10ml na 1mol/L HCl, spectrophotometer, 430nm, 10mm kauri.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml na 0.01mol/L HCl
Ammonium (NH4): B-2
Sulfate (SO4): 1.2g, (1), ref: 0.50ml na 0.005mol/L H2SO4
Iron (Fe): 1.5g, (2), Ref: 1.5ml na Iron Std.(0.01mg/ml)
Karfe masu nauyi (Pb): 2.0g, (2), Ref: 2.0ml na Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (2), ref: 2.0ml na As2O3 Std.
Sauran Amino Acids: Tsarma ammonia R2 Samfurin Gwajin: 50μg, F-6-a, sarrafawa: L-Tyr 0.25μg
Asarar bushewa: a 105 ℃ na 3 hours.
Residue on Ignition (Sulfated): AJI Test 13
Assay: samfurin bushe, 180mg, (1), 6ml na formic acid, 50ml na glacial acetic acid, 0.1mol/L HCLO4 1ml=18.119mg C9H11NO3
Gwajin pH: 0.02g a cikin 100ml cikakken bayani mai ruwa

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) Hanyar Gwajin USP35
BAYANI
Tyrosine ya ƙunshi NLT 98.5% da NMT 101.5% na L-Tyrosine (C9H11NO3), ƙididdiga akan busasshen tushe.
GANO
A. RASHIN NUTSUWA <197K>
ASSAY
TSARI
Misali: 180 MG na Tyrosine
Blank: Mix 6 ml na formic acid da 50 ml na glacial acetic acid.
Titrimetric tsarin
(Duba Titrimetry <541>)
Yanayin: Kai tsaye titration
Titrant: 0.1 N perchloric acid VS
Gano ƙarshen ƙarshen: Potentiometric
Analysis: Narkar da Samfurin a cikin 6 ml na formic acid da 50mL na glacial acetic acid, da titrate tare da Titrant.Yi ƙudurin Blank.
Yi lissafin adadin tyrosine (C9H11NO3) a cikin Samfurin da aka ɗauka:
Sakamako = {[(VS-VB) x N x F]/W} x100
VS = ƙarar Titrant da Samfurin (mL) ya cinye
VB = ƙarar Titrant da Blank (mL) ke cinyewa
N = ainihin al'ada na Titrant (mEq/ml)
F= ma'aunin daidai, 181.2 mg/mEq
W= Nauyin Nauyin (mg)
Sharuɗɗan karɓa: 98.5% ~ 101.5% akan busasshiyar tushe
RASHIN ZUCIYA
RASHIN WUTA <281>: NMT 0.4%
chloride da sulfate, Chloride <221>
Daidaitaccen bayani: 0.20ml na 0.020 N hydrochloric acid
Misali: 0.35 g na Tyrosine.[NOTE-Idan ya cancanta, narke Samfurin ta dumama zuwa kusa da tafasa da ƙara 1 ml na nitric acid.]
Sharuɗɗan karɓa: NMT 0.04%
chloride da sulfate, sulfate <221>
Daidaitaccen bayani: 0.50mL na 0.020 N sulfuric acid
Misali: 1.2 g na Tyrosine.[NOTE-Idan ya cancanta, narkar da Samfurin ta ƙara 6 ml na diluted hydrochloric acid.]
Sharuɗɗan karɓa: NMT 0.04%
IRON <241>: NMT 30ppm [NOTE-Idan ya cancanta, yi amfani da 2 ml na hydrochloric acid don narkar da Samfurin.]
KARFE MAI KYAU, Hanyar I <231>: NMT 15ppm
Abubuwan da suka danganci
Diluted ammonia bayani: Tsarma 16 ml na mayar da hankali ammonia da ruwa zuwa 100 ml.
Maganin dacewa da tsarin: Narkar da 10 MG na USP L-Tyrosine RS da 10 MG na USP L-Phenylalanine RS a cikin 1 mL na Diluted ammonia bayani, kuma tsarma da ruwa zuwa 25.0 ml.
Daidaitaccen Magani: A cikin flask mai dacewa, narkar da adadin USP L-Tyrosine RS a cikin 1 ml na maganin ammonia Diluted.Tsarma da ruwa zuwa ƙara don samun bayani na 0.05mg/mL.[NOTE-Wannan bayani yana da maida hankali daidai da 0.5% na na samfurin bayani.]
Samfurin bayani: Canja wurin 0.1 g na Tyrosine a cikin 10-mL volumetric flask, ƙara isasshe Diluted ammonia bayani don narke, da kuma tsarma da ruwa zuwa girma.
Tsarin Chromatographic (Duba Chromatography <621>, Chromatography na bakin-Layer.)
Yanayin: TLC
Adsorbent: 0.25-mm Layer na chromatographic silica gel cakuda
Girman aikace-aikacen: 5μL
Haɓaka tsarin ƙarfi: isopropyl barasa da ammonium hydroxide (7: 3)
Fesa reagent: 2 mg/mL na ninhydrin a cikin cakuda butyl barasa da 2N acetic acid (95:5)
Dacewar tsarin
Bukatun dacewa: chromatogram na tsarin dacewa da tsarin yana nuna aibobi biyu a sarari.
Bincike
Samfura: Maganin dacewa da tsarin, daidaitaccen bayani, da Samfurin bayani.
Bushe farantin tsakanin 100° da 105° har sai ammonia ya ɓace gaba ɗaya.Fesa da Reagent Fesa, kuma zafi tsakanin 100 ° da 105 ° na 15 min.Yi nazarin farantin a ƙarƙashin farin haske.
Sharuɗɗan karɓa: Duk wani wuri na biyu na samfurin samfurin bai fi girma ko mafi tsanani fiye da ainihin tabo na daidaitaccen bayani ba.
Kowane kazanta: NMT 0.5%
Jimlar ƙazanta: NMT 2.0%
MAKAMMAN JARRABAWA
Juyawa ta gani, Jujjuya ta musamman <781S>
Maganin samfurin: 50 mg/mL a cikin 1 N hydrochloric acid
Sharuɗɗan karɓa: -9.8° zuwa -11.2°
RASHIN BUSHEWA <731>: bushe samfurin a 105 ℃ na 3 h: yana asarar NMT 0.3% na nauyinsa.
KARIN BUKATA
KYAUTA DA AJIYA: Ajiye a cikin kwantena da aka rufe sosai.
MATSAYIN NAZARI NA USP <11>
USP L-Phenylalanine RS
USP L-Tyrosine RS