Kayan aiki: Kayan aikin GC (Shimadzu GC-2010)
Shafin: DB-17 Agilent 30mX0.53mmX1.0μm
Zafin tanda na farko: 80 ℃
Lokacin farko 2.0min
Matsakaicin 15 ℃/min
Zafin tanda na ƙarshe: 250 ℃
Lokacin ƙarshe 20min
Mai ɗaukar iskar Nitrogen
Yanayi Constant kwarara
Guda 5.0ml/min
Raba rabo 10:1
Injector zafin jiki: 250 ℃
Zazzabi mai ganowa: 300 ℃
Girman allura 1.0μL
Kariyar da ya kamata a yi kafin bincike:
1. Yanayin yanayin a 240 ℃ don mafi ƙarancin 30minutes.
2. A wanke sirinji da tsaftataccen layin allura da kyau don cire gurɓatattun binciken da aka yi a baya.
3. A wanke, bushe da cika diluent a cikin kwalabe na sirinji.
Shirye-shiryen diluent:
Shirya 2% w/v sodium hydroxide bayani a cikin ruwa.
Daidaitaccen shiri:
Auna kimanin 100mg na (R) -3-hydroxyprolidine hydrochloride misali a cikin vial, ƙara 1mL na diluent kuma narkar da.
Shirye-shiryen gwaji:
Auna kimanin 100mg na samfurin gwaji a cikin vial, ƙara 1ml na diluent kuma narke.Shirya cikin kwafi.
Tsari:
Allurar blank (diluent), daidaitaccen shiri da shirye-shiryen gwaji ta amfani da yanayin GC na sama.Yi watsi da kololuwa saboda babu komai.Lokacin riƙewa na kololuwa saboda (R) -3-hydroxyprolidine shine kusan 5.0min.
Lura:
Bayar da sakamakon a matsayin matsakaici
Lokacin aikawa: Nuwamba-13-2021