Phthalimide Potassium Salt CAS 1074-82-4 Tsafta > 99.0% (HPLC)

3. Hanyar gwaji
3.1 Asara akan bushewa (W/W)
3.1.1 Kayan aiki da kayan aiki
Sama da dubu goma na ma'auni na lantarki, tanda mai bushewa mai zafi na lantarki, kwalban awo 40*25, na'urar bushewa
Reagent: silica gel
3.1.2 Hanyoyi
1. Tsaftace kwalabe masu auna 3 40*25.
2, saka a cikin tanda a 105 ℃ (± 2 ℃) bushe don 1 hour.
3. Ki fitar da kwalbar awo 40*25 ki saka a cikin injin bushewa ya huce na tsawon mintuna 30.
4, Ma'auni daidai 40*25 kwalban auna mara nauyi W0.
5. Yi amfani da kwalban auna 40 * 25 don auna samfurin da aka haɗe daidai da 3.0000g (W1), jimlar 3.
6, Saka a cikin tanda, cire hular kwalban, sanya shi kusa da kwalban awo, ko rabin bude kwalban kwalban don bushewa, cirewa, dole ne ya kasance mai kyau auna kwalban, bushe a 105 ℃ (± 2℃) don 3 hours.
7. Cire kwalban awo 40*25 kuma saka shi a cikin na'urar bushewa don kwantar da hankali na 30min.
8, Ma'auni daidai bayan bushewa 40 * 25 nauyin kwalban W2.
Ƙididdigar ƙididdiga: Asara akan bushewa (%) = (W1-W2)/(W1-W0)×100%
3. 4 Ƙaddamar da abun ciki
3.4.1 Kayan aiki da kayan aiki
LC-UV100 Liquid Chromatograph (Shanghai Wufeng Scientific Instrument Co., LTD.)
10ml volumetric kwalban, bakin karfe kusurwa cokali da sauran related wurare
3.3.2 Hanyoyi
Hanyar bincike na Phthalimide Potassium Salt
3.3.3 Liquid chromatography
Halin chromatographic
Shafin Chromatographic: HypersilC6H5 (C8 kuma akwai) 4.6*250mm 5um
Tsawon Gano: 210nm
Yanayin zafin jiki: 30 ℃
Yawan gudu: 1.0ml/min
Samfurin maida hankali: 0.05mg/ml (diluent shine lokacin wayar hannu)
Reagents
Potassium Phosphate Dibasic (tsarki na nazari)
Phthalimide Potassium Gishiri (misali misali)
Acetonitrile (tsarki na nazari)
Ruwa mai tsafta
Lokacin wayar hannu: acetonitrile: buffer phosphate (PH:3.5) =40:60
Auna 1.63g Potassium Phosphate Dibasic kuma narke a cikin ruwa mai tsabta na 600ml, daidaita darajar PH zuwa 3.5 tare da phosphoric acid, zuba cikin 1000ml beaker dauke da 400ml acetonitrile, Mix da kyau, tace tare da 0.45um gauraye fiber tace membrane, narke da kuma tsayawa tare da ultrasonic vibration. aikace-aikace.
3.3.4 Tsari
1) Maganin gwajin saiti (0.05mg/ml)
An auna samfurin 2.5mg daidai kuma an saka shi a cikin kwalban volumetric 50ml.An ƙara lokacin wayar hannu kuma an diluted zuwa sikelin ta hanyar rushewar ultrasonic.
2) Maganin daidaitawa (1.0mg/ml)
An auna ma'auni na 2.5mg daidai kuma an saka shi a cikin kwalban volumetric 50ml.An ƙara lokacin wayar hannu kuma an diluted zuwa sikelin ta hanyar rushewar ultrasonic.
3) Amfani da tsarin:
(1) Sanya kan tacewa a cikin methanol 80% yayin farawa, buɗe bawul ɗin shayewa da magudana don 3-5min, sannan rufe bawul ɗin shayewa kuma kurkura na 10-15min.Sai ki zuba kan tace a cikin ruwa mai tsafta kashi 90%, sai ki bude magudanar ruwa ki sauke na tsawon mintuna 3-5, sai ki rufe magudanar ruwa ki wanke na tsawon minti 10.
(2) Sa'an nan kuma canza tsarin wayar hannu da aka shirya, ƙimar kwararar ita ce 1.0ml/min, matsi na gaban shafi bai wuce 1000Psi ba, buɗe na'urar ganowa, jira har sai matsi na gaba na shafi ya tabbata, buɗe wurin aikin kwamfuta, sannan shigar da kan layi.
(3) Bayan ginshiƙi na gaba ya tsaya tsayin daka, buɗe mai ganowa.Da farko a yi allura 1 na lokacin wayar hannu, sannan a yi allura 1 na daidaitaccen samfurin, sannan a yi samfurin.
(4) Sakamakon lissafin: Hanyar daidaita yanki
(5) Bayan kammala aikin bincike, rufe mai ganowa da farko, sa'an nan kuma kurkura kan tacewa a cikin ruwa mai tsabta don 10-15min;A lokaci guda, an wanke bawul ɗin allurar da ruwa da methanol.
(6) A ƙarshe, sanya methanol 80%, kurkure na 30min, sannan a rufe.
4. Bayan kammala dubawa, cika takardar dubawa da gwajin gwaji.