Adenosine CAS 58-61-7 Assay 99.0%-101.0% USP Hale Hana Maʻemaʻe Kiʻekiʻe

HOAKAKA
Aia ka Adenosine i ka NLT 99.0% a me NMT 101.0% o C10H13N5O4, i helu ʻia ma ke kumu maloʻo.
IKE
• A. KA HOʻOPIʻI INFRARED <197K>: NMT 0.1%
HANA
• ADENOSINE
Laʻana: 200 mg o Adenosine i maloʻo mua ma 105° no 2h
Pūnaehana Titrimetric
(E nānā i ka Titrimetry <541>)
Ke ʻano: Hoʻohālikelike pololei
Titrant: 0.1 N no ka ʻakika chloric VS
ʻImi hope: Potentiometric
Nānā: E hoʻoheheʻe i loko o 50 mL o ka ʻakika acetic glacial a e hoʻokaʻawale me 0.1 N no kēlā me kēia waikawa chloric VS.E helu i ka pākēneka o Adenosine (C10H13N5O4) ma ka ʻāpana i lawe ʻia:
Ka hopena = [(V − B) × N × F × 100]/W
V = Laʻana titrant volume (mL)
B = Ka nui titrant pāʻani (mL)
N= ka mea maʻamau titrant (mEq/mL)
F= helu kaulike: 267.25 mg/mEq
W= kaumaha o ka Laʻana (mg)
Nā pae hoʻoholo: 99.0% -101.0% ma ke kumu maloʻo
• NA MEA HELE
• KA HOʻOPIʻI KOE <281>: NMT 0.1%
• NA METAL HEAVY, Hana II <231>: NMT 10 ppm
• LIMIT O AMMONIA
Laʻana hoʻonā: Hoʻokuʻu i 0.5 g i 10 mL o ka wai.E hoʻolālā no 30 s, a hele i kahi kānana koʻikoʻi.E hoʻoheheʻe i ka filtrate me ka wai i 15 mL, a hoʻohana i ka kānana.
Haʻina maʻamau: 0.4 µg/mL o ka ammonium chloride i loko o ka wai
Hoʻopili: E hoʻohui i 0.3 mL o ka alkaline mer curic-potassium iodide TS i loko o ka hāpana laʻana, e uhi i nā paipu hoʻāʻo, a e kū no 5 min.
Nā pae hoʻoholo: ʻAʻole hōʻike ʻia ka waihoʻoluʻu laʻana i kahi waihoʻoluʻu melemele ʻoi aku ka ikaika ma mua o ka hopena maʻamau (NMT 4 ppm o ka amonia).
• KA LAHI O KLORIDA
Laʻana hoʻonā: Hoʻokuʻu i 0.2 g i 10 mL o ka wai.E hoʻoulu no 30 s, e hele i kahi kānana koʻikoʻi, a hoʻohana i ka kānana.
ʻO ka hopena maʻamau: 2.3 µg/mL o ka sodium chloride i ka wai
Nānā: E hoʻohui i 1 mL o ka waikona nitric a me 1 mL o ka nitrate kālā TS i ka hāpana laʻana a me 10 mL o ka solution Standard, a hoʻoheheʻe i kēlā me kēia hopena me ka wai i 40 mL.E ʻae i nā haʻina e kū no 5 min, pale ʻia mai ka mālamalama.
Nā pae ʻae ʻae: Ke nānā ʻia me kahi ʻeleʻele ʻeleʻele, ʻaʻole ʻoi aku ka ʻino o ka solution Sample ma mua o ka solution Standard (NMT 0.007% chloride).
• KA LAHI O KA SULFATE
Laʻana hoʻonā: Hoʻokuʻu i 0.75 g i 15 mL o ka wai.E hoʻoulu no 30 s, e hele i kahi kānana koʻikoʻi, a hoʻohana i ka kānana.
Haʻina maʻamau: E hoʻohui i ka 0.15 mL o 0.020 N sulfuric acid i 15 mL o ka wai.
Nānā: E hoʻohui i ka hāpana laʻana a me ka solution Standard i 2 mL o ka barium chloride TS a me 1 mL o 3 N hydrochloric acid, e hoʻoheheʻe i kēlā me kēia hopena me ka wai i 30 mL, a hui.E ʻae i nā haʻina e kū no 5 min.
Nā pae hoʻohālikelike: ʻAʻole ʻoi aku ka ʻino o ka hāpana laʻana ma mua o ka solution Standard (NMT 0.02% sulfate).
• NA MEA HOOMAEMAE
Pane A: 6.8 g/L o ka potassium hydrogen sulfate a me 3.4g/L o ka tetrabutylammonium hydrogen sulfate i loko o ka wai.Hoʻoponopono me ka 2 N pāhareha waihā i kahi pH o 6.5.
Pane B: 0.1 g/L o ka solution sodium azide
Māhele paʻa lima: Haʻina A a me B (60:40)
ʻO ka hopena kūpono o ka ʻōnaehana: 0.2 mg/mL kēlā me kēia Adenosine a me ka inosine i ka pae Mobile
Laʻana hoʻonā: 1.0 mg/mL o Adenosine i ka pae Mobile
Pūnaehana Chromatographic
(E nana Chromatography <621>, Pūnaehana Pono.)
Ke ano: LC
ʻIke: UV 254 nm
kolamu: 4.6-mm × 25-cm;5-µm paʻi L1
Kahe kahe: 1.5 mL / min
Ka nui o ka hoʻopio: 20 µL
Kūpono ʻōnaehana
Nā Laʻana: Haʻina kūpono ʻōnaehana
Nā koi kūpono
Hoʻoholo: NLT 9.0 ma waena o adenosine a me inosine
ʻO ke kumu huelo: NMT 2.5
Wehewehe maʻamau pili: NMT 2.0%
[NOTE-Chromatograph the Sample solution, a hoʻololi i ka manawa holo i ʻelua paha o ka manawa paʻa o ka piko nui.]
Kānāwai
Laʻana: Laʻana hoʻonā
E helu i ka pākēneka o kēlā me kēia haumia ma ka ʻāpana o Adenosine i lawe ʻia:
Ka hopena = (rU/rT) × 100
rU = pane kiʻekiʻe o kēlā me kēia haumia mai ka Haʻina Haʻawina
rT = huina o na pane a pau no na piko a pau mai ka Haʻina Haʻawina
Nā pae ʻae ʻae
Nā haumia o kēlā me kēia kanaka: NMT 0.1% kēlā me kēia guanosine, inosine, a me ka uridine, a me NMT 0.2% o adenine
Huina haumia: NMT 0.5%
NA HOAO KUAI
• KA HELE HEHEHE A I OLE KA MAHELE <741>: 233°-238°
• KA HOOLOLI OPTICAL, Kawili kiko'ī <781S>: -68° a i -72°
Haʻina hoʻāʻo: 20 mg/mL i ka solution sodium hydroxide (1 i ka 20), i hoʻoholo ʻia ma kahi hāpana i maloʻo mua ma 105° no 2h.
KA HIKI A I ʻole ALKALINITY: E hoʻokuʻu i 1 g i loko o 20 mL o ka wai kalapona kalapona ʻole.E hoʻolālā no 30 s, a hele i kahi kānana koʻikoʻi.I kēlā me kēia ʻelua ʻāpana 10-mL o ka kānana e hoʻohui i 0.1mL o ka bromocresol poni TS.
Pono e ʻae ʻia: NMT 0.3 mL o 0.01 N sodium hydroxide pono e hana i kahi waihoʻoluʻu uliuli-violet i hoʻokahi ʻāpana.NMT 0.1 mL o 0.01 N hydrochloric acid pono e hana i ka waihoʻoluʻu melemele ma ka ʻāpana ʻē aʻe.
LOSSON DRYING <731>: Hoʻomaloʻo i kahi hāpana ma 105 ℃ no 2 mau hola: nalowale NMT 0.5% o kona kaumaha.
NA KOI HOU
• PACKAGING a me ka mālama ʻana: mālama i loko o nā pahu paʻa a me ka māmā, a mālama ʻia ma ka lumi wela.
• USP REFERENCE STANDARD <11>
USP Adenosine RS