L-Glutamic Acid α-Benzyl Ester CAS 13030-09-6 (H-Glu-OBzl) Assay >98.5% (HPLC) Mea Kiʻekiʻe

1. Maʻemaʻe (HPLC)
1.1 Mea Hana
Kiʻekiʻe hana wai chromatography, UV3000 detector.
Kaulike analytical electronic
1.2 Reagents
Acetonitrile (papa chromatographic), TFA (papa chromatographic)
1.3 Nā kūlana Chromatographic
1.3.1 kolamu: kolamu C18, 5 μl * 250 * 4.6 mm
1.3.2 Ka lōʻihi nalu ʻike: 220nm
Kahe kahe: 1.0 mL / min
Ka nui o ka injection: 10 μL (no ka ʻike)
Mea hoʻoheheʻe: acetonitrile
Ka manawa holo: 20 minuke
1.4 Ka hoʻomākaukau ʻana o ka pae kelepona
Mobile phase A (0.1% Trifluoroacetic acid Water): e hoʻopili pono i ka 1.0 ml TFA i hoʻoheheʻe ʻia i 1000 ml me ka wai, hui a me ka degas.
Mobile phase B (0.1% Trifluoroacetic acid Acetonitrile): hoʻopili pono i ka 1.0 ml TFA, hoʻoheheʻe ʻia i 1000 ml me ka acetonitrile, hui a me ka degas.
1.5 Papahana Gradient
Manawa (min) A% B%
0:00 90 10
10.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
1.6 Hoʻomākaukau i ka hāpana hāpana
Kauona 0.1 g hāpana a hoʻoheheʻe ia me ka acetonitrile a dilute ia i 100ml.E lulu maikaʻi no ka hoʻohana ʻana i ka wā e hiki mai ana, a i ʻole ka manaʻo like.E hoʻomākaukau i ʻelua laʻana i ka like.
1.7 Hoʻoholo Laʻana
E noʻonoʻo i ka hāpana e like me ke kaʻina hana laʻana:
1) ʻOi aku ma mua o 1 injection o ka hāʻina blank
2) 1 inikia o ka hāpana hāpana 1#
3) 1 inikia o ka hāpana hāpana 2#
1.8.0 Heluhelu
1.8.1 Ua hoʻohana ʻia ke ʻano o ka hoʻomaʻemaʻe ʻana o ka wahi kiʻekiʻe e helu i ka maʻemaʻe o ka HPLC.
1.8.2 ʻAʻole ʻoi aku ka nui o ka hoʻokaʻawale ʻana o ka maʻemaʻe o nā nila ʻelua ma mua o 1%
1.8.3 Inā kūpono nā hopena o nā inikini ʻelua i nā koina ʻae, e lawe i ka maʻemaʻe maʻemaʻe ma ke ʻano he hopena hope loa.