Vardenafil Intermediate CAS 224789-21-3 Maʻemaʻe> 99.0% (HPLC)

1. Nānā: E kau i ka hāpana ma ka pepa keʻokeʻo a nānā i ke ʻano, keʻokeʻo a hiki i ka pauka melemele ʻeleʻele.
2. Nānā:
2.1 Hoʻoholo ʻia ka helu heheʻe e like me ke kānāwai (Pharmacoeia Chinese 2010 Edition II Appendix ⅥC) He 142.0 ~ 148.0 ℃ ka helu heheʻe.
2.2 Pohō ma ka hoʻomaloʻo ʻana: Ka hoʻoholo ʻana e like me ke kānāwai (Appendix ⅧL, Part II, 2010 Edition of Chinese Pharmacopoeia).ʻO ke kaupaona ʻana i ka nui kūpono o kēia huahana, maloʻo ma 102 ℃ no 3 mau hola, ʻaʻole e ʻoi aku ke kaumaha ma mua o 1.00%.
2.3 Nā Metala Kaumaha: Hoʻoholo e like me ke kānāwai (People's Republic of China Pharmacopoeia 2010 Edition, Part II Appendix ⅧH) Ka hoʻoholo ʻana ma ke ʻano ʻelua.
Precision kaupaona 1.0g o ka hoao ana huahana, lohi wela a hiki loa carbonized, anuanu, e hoʻohui 0.5-1.0ml sulfuricʻakika, hana ia mea maʻemaʻe, wela i ka haʻahaʻa wela a hiki i ka sulfuricʻakika ua pau, hoʻohui i 0.5ml nitric acid, mahu maloʻo, a hiki i ua pau ka naikokene oxide vapor, wela, wela ma 500-600 ℃ i carbonized loa, hooluolu, hoʻohui i 2ml hydrochloric acid, Ma hope o ka maloʻo ʻana i loko o ka wai ʻauʻau, hoʻohui i ka wai 15ml, e hoʻokuʻu i ka hopena hoʻāʻo ammonia a hiki i ka lilo ʻana i mea kū ʻole i ka hōʻailona phenolphthalein. ka hopena, e hoʻohui i ka 2ml acetate buffer (ph3.5), a hoʻoheheʻe iki i ka wela, e neʻe i ka Nachner colorimetric tube, a hoʻoheheʻe ʻia i loko o 25ml me ka wai.E lawe hou i ka reagent no ka hoʻomākaukau ʻana i ka hopena hoʻāʻo, a e mahu i loko o kahi kīʻaha porcelain, e hoʻohui i ka 2ml acetate buffer (PH3.5) a me ka wai 15ml e wela iki Ma hope o ka hemo ʻana, e hoʻoneʻe i ka tube colorimetric Nath, e hoʻohui i kahi nui o ka hopena alakaʻi maʻamau, a laila hoʻoheheʻe me ka wai i 25ml.Hoʻokō ʻia ka hoʻokolohua colorimetric ma waena o ka hopena hoʻāʻo a me ka hopena maʻamau e loaʻa ai.Hoʻomākaukau hoʻonā maʻamau e like me (People's Republic of China Pharmacopoeia 2010 edition II Appendix Ⅷ Heavy metal test method) ke ʻano mua.ʻAʻole pono nā metala kaumaha ma mua o 20 mau ʻāpana o ka miliona.
3. Maʻemaʻe: E like me ka hana kiʻekiʻe wai chromatography, i hoʻoholo ʻia e like me ke kānāwai (Chinese Pharmacopoeia 2010 edition II Appendix VD).
Nā kūlana Chromatographic:
Kona: C18, 200mm×4.6mm, 5μm;
ʻO ka pae kelepona: Phosphate buffer (kaumaha 2.72g potassium dihydrogen phosphate, hoʻohui i ka wai e hoʻoheheʻe a hoʻoheheʻe i 1000ml, hoʻoponopono i ka pH=3.5): acetonitrile: = 35:65;
Ka lōʻihi o ka nalu ʻike: 254nm;
Kahe kahe: 1.0ml / min.
Kaʻina hoʻoholo: E lawe ma kahi o 30mg o kēia huahana, e kaupaona pololei, e hoʻoheheʻe ʻia me ka pae kelepona e hana i ka manaʻo o kahi 0.3mg/ml, e lawe i 20μl i loko o ka chromatograph wai, e hoʻopaʻa i ka chromatogram.Wahi a ke ʻano hana maʻamau, ʻaʻole emi ka maʻemaʻe ma mua o 99.0%.