Chenodeoxycholic Acid (CDCA) CAS 474-25-9 Assay ≥98% (Dry Basic)

Chenodeoxycholic Acid
C24H40O4: 392.57
3a,7a-Dihydroxy-5b-cholan-24-oic acid [474-25-9]
ʻO ka Chenodeoxycholic Acid, ke maloʻo, ʻaʻole i emi iho ma lalo o 98.0z a ʻaʻole ʻoi aku ma mua o 101.0% o C24H40O4.
Hōʻike ʻia ʻo Chenodeoxycholic Acid e like me ke keʻokeʻo, nā kristal, ka pauka crystalline a i ʻole ka pauka.
Hiki ke hoʻoheheʻe ʻia i ka methanol a me ka ethanol (99.5), hiki ke hoʻoheheʻe ʻia i ka acetone, a hiki ʻole ke hoʻoheheʻe ʻia i ka wai.
Identification E hoʻoholo i ke kikoo infrared absorption spectrum o Chenodeoxycholic Acid, i hoʻomaloʻo mua ʻia, e like me ke kuhikuhi ʻana ma ke ʻano pāpaʻi pāpaʻa bromide ma lalo o Infrared Spectrophotometry <2.25>, a hoʻohālikelike i ke kikoʻī me ka Reference Spectrum: hōʻike nā kikoʻī ʻelua i ka ikaika like o ka absorption ma nā helu nalu like.
ʻO ka hoʻololi ʻana o ka Optical <2.49> [a]20D : +11.0 °~ +13.0° (ma hope o ka maloʻo ʻana, 0.4 g, ethanol (99.5), 20 mL, 100 mm).
Ka helu hehee <2.60> 164~169 ℃
Maʻemaʻe (1) Chloride <1.03>-E hoʻoheheʻe i 0.36 g o ka Chenodeoxycholic Acid i 30 mL o ka methanol, e hoʻohui i 10 mL o ka wai nitric dilute a me ka wai e hana i 50 mL, a hana i ka hoʻāʻo me kēia hoʻonā.E hoʻomākaukau i ka hopena hoʻomalu penei: I ka 1.0 mL o ka 0.01 mol/L hydrochloric acid VS e hoʻohui i 30 mL o ka methanol, 10 mL o ka waikawa nitric dilute a me ka wai e hana ai i 50 mL (ʻaʻole ʻoi aku ma mua o 0.1%).
(2) Nā metala kaumaha <1.07> -E hoʻomau me 1.0 g o Chenodeoxycholic Acid e like me ke ʻano 4, a hana i ka hoʻāʻo.E hoʻomākaukau i ka hoʻonā hoʻomalu me 2.0 mL o ka ʻOi (ʻaʻole ʻoi aku ma mua o 20 ppm).
(3) Barium-I 2.0 g o Chenodeoxycholic Acid e hoʻohui i 100 mL o ka wai, a hoʻolapalapa no 2 mau minuke.I kēia hoʻonā e hoʻohui i 2 mL o ka waika hydrochloric, e hoʻolapalapa no 2 mau minuke, kānana ma hope o ka hoʻoluʻu ʻana, a holoi i ka kānana me ka wai a hiki i ka 100 mL o ka kānana.I ka 10 mL o ka kānana e hoʻohui i 1 mL o ka waika sulfuric dilute: ʻaʻohe ʻike ʻia ka turbidity.
(4) Nā mea pili-E hoʻoheheʻe i 0.20 g o Chenodeoxycholic Acid i loko o ka hui ʻana o ka acetone a me ka wai (9: 1) e hana pono i 10 mL, a hoʻohana i kēia hāpana e like me ka hāpana hāpana.E hoʻokaʻawale, e hoʻoheheʻe i 10 mg o ka lithocholic acid no ka chromatography thin-layer i ka hui ʻana o ka acetone a me ka wai (9:1) e hana pono i 10 mL.Pipet 2 mL o kēia hāpana, hoʻohui i ka hui ʻana o ka acetone a me ka wai (9:1) e hana pono i 100 mL, a hoʻohana i kēia ʻano hoʻonā e like me ka hopena maʻamau (1).E hoʻokaʻawale, e hoʻoheheʻe i 10 mg o ka ursodeoxycholic acid i ka hui ʻana o ka acetone a me ka wai (9:1) e hana pono i 100 mL, a hoʻohana i kēia hopena e like me ka hopena maʻamau (2).E hoʻokaʻawale, e hoʻoheheʻe i 10 mg o ka waikawa cholic no ka chromatography thin-layer i loko o ka hui ʻana o ka acetone a me ka wai (9:1) e hana pono i 100 mL, a hoʻohana i kēia hopena e like me ka hopena maʻamau (3).Pipet 1 mL o ka hāpana hāpana, a hoʻohui i ka hui ʻana o ka acetone a me ka wai (9:1) e hana pono i 20 mL.Pipet 0.5 mL, 1 mL, 2 mL, 3 mL a me 5 mL o keia solution, e hookui i ka hui ana o ka acetone a me ka wai (1) i kela mea keia mea e hana pono i 50 mL, a e koho i keia mau ha'ina e like me ka solution ma'amau A, solution ma'amau. B, hoʻonā maʻamau C, hāʻina maʻamau D a me ka hāʻina maʻamau E, kēlā me kēia.E hana i ka ho'āʻo me kēia mau hoʻonā e like me ka kuhikuhi ʻana ma lalo o Thin-layer Chromatography<2.03>.E kau i 5 mL o kēlā me kēia hāpana hāpana, nā hāʻina maʻamau (1), (2), (3) a me nā hāʻina maʻamau A, B, C, D a me E ma luna o ka pā silica gel no ka chromatography lahilahi.E hoʻomohala i ka pā me ka hui ʻana o 4-methyl-2-pentanone, toluene a me formic acid (16:6:1) i kahi mamao ma kahi o 15 cm, e hoʻomaloʻo i ka pā, a maloʻo hou ma 1209C no 30 mau minuke.E kāpī koke i ka hopena o ka waikawa phosphomolybdic n-hydrate i loko o ka ethanol (95) (1 i ka 5) ma ka pā, a me ka wela ma 120 ℃ no 2 a 3 mau minuke: kahi e pili ana i kahi me ka hopena maʻamau (1) ʻaʻole i ʻoi aku ka ikaika ma mua o ke kiko me ka hoʻonā maʻamau (1), ʻaʻole i ʻoi aku ka ikaika o ke kiko e pili ana i ke kiko me ka hopena maʻamau (2) ma mua o ke kiko me ka hopena maʻamau (2), a me ke kiko e pili ana i ke kiko. me ka hoʻonā maʻamau (3) ʻaʻole i ʻoi aku ka ikaika ma mua o ka wahi me ka hopena maʻamau
(3).I ka hoʻohālikelike ʻana i nā kiko me nā hāʻina maʻamau A, B, C, D a me E, ʻaʻole i ʻoi aku ka ikaika o nā kiko ma waho o ke kiko nui a me nā kiko i ʻōlelo ʻia ma luna aʻe ma mua o ke kiko me ka solution maʻamau E, a me ka huina nui. ʻaʻole ʻoi aku ka nui o lākou ma mua o 1.5%.
ʻAʻole i ʻoi aku ma mua o 1.5% (1 g, 105℃,3 hola).
ʻAʻole i ʻoi aku ma mua o 0.1% (1 g).
E kaupaona pololei ma kahi o 0.5 g o Chenodeoxycholic Acid, i hoʻomaloʻo mua ʻia, e hoʻoheheʻe i 40 mL o ka ethanol (95) a me 20 mL o ka wai, a e hoʻokaʻawale i <2.50> me 0.1 mol/L sodium hydroxide VS (potentiometric titration).E hana i kahi hoʻoholo hakahaka ma ke ʻano like, a hana i nā hoʻoponopono e pono ai.
ʻO kēlā me kēia mL o 0.1 mol/L sodium hydroxide VS ＝ 39.26 mg o C24H40O4
ʻO nā pahu a me ka mālama ʻana i nā pahu pahu-paʻa paʻa.