Citric Acid Anhydrous CAS 77-92-9 Assay 99.5~100.5%

Citric acid [77-92-9].
HOAKAKA
Anhydrous Citric Acid loaʻa ka NLT 99.5% a me ka NMT 100.5% o C6H8O7, i helu ʻia ma ke kumu anhydrous.
IKE
• Infrared Absorption <197K>: Hoʻomaloʻo i ka mea e nānā ʻia ma 105 ℃ no 2 hola.
HANA
• Kaʻina hana
Laʻana: 0.550 g o Anhydrous Citric Acid;hoʻopaʻa pono i ke kaumaha.
Nānā: E hoʻoheheʻe i ka Laʻana i 50 mL o ka wai.E hoʻohui i 0.5 mL o ka phenolphthalein TS.Titrate me 1 N sodium hydroxide VS.ʻO kēlā me kēia mL o 1 N sodium hydroxide ua like ia me 64.03 mg o C6H8O7.
Nā pae hoʻohālikelike: 99.5% -100.5% ma ke kumu anhydrous
NA mea haumia
Nā mea haumia ʻole
• Ke koena ma ka Ignition <281>: NMT 0.1%, hoʻoholo ʻia ma 1.0 g
• Nā Metala Kaumaha <231>: NMT 10 ppm
• Sulfate
ʻO ka solution sulfate maʻamau A: 1.81 mg/mL o ka pāpaʻi sulfate i 30% waiʻona.Ma mua koke o ka hoʻohana ʻana, e hoʻololi i 10.0 mL o kēia hopena i kahi pahu volumetric 1000-mL, e hoʻoheheʻe me 30% waiʻona i ka nui, a hui pū.Loaʻa i kēia hopena he 10 µg/mL o ka sulfate.
ʻO ka solution sulfate maʻamau B: 1.81 mg/mL o ka pāpaʻi sulfate i loko o ka wai.Ma mua koke o ka hoʻohana ʻana, e hoʻololi i 10.0 mL o kēia hopena i loko o kahi pahu volumetric 1000-mL, e hoʻoheheʻe me ka wai i ka nui, a hui pū.Loaʻa i kēia hopena he 10 µg/mL o ka sulfate.
Laʻana waihona waihona: 66.7 mg/mL o ka waikawa citric
Laʻana hoʻonā: I ka 4.5 mL o ka mea hoʻonā sulfate maʻamau A, hoʻohui i 3 mL o kahi solution barium chloride (1 in 4), lulu, a ʻae e kū no 1 min.I ka 2.5 mL o ka hopena o ka hoʻokuʻu ʻana, e hoʻohui i 15 mL o ka Sample stock solution a me 0.5 mL o 5 N acetic acid, a hui.
Haʻina maʻamau: E hoʻomākaukau e like me ke kuhikuhi ʻana no ka hāʻina Laʻana, koe naʻe ka hoʻohana ʻana i 15 mL o ka solution sulfate maʻamau B ma kahi o ka solution stock laʻana.
Kānāwai
Nā Laʻana: Haʻina maʻamau a me ka hāʻina Laʻana
Nā pae hoʻoholo: ʻAʻole ʻoi aku ka nui o kēlā me kēia turbidity i hana ʻia i ka hopena laʻana ma hope o 5 min e kū nei ma mua o ka mea i hana ʻia ma ka solution Standard (0.015%).
• Ka palena o ka Aluminum (kahi i kapa ʻia e like me ka mea i manaʻo ʻia no ka hoʻohana ʻana i ka dialysis)
ʻO ka solution alumini maʻamau: I ka 352 mg o ka alumini potassium sulfate i loko o kahi pahu volumetric 100-mL, e hoʻohui i kekahi mau mL o ka wai, e hoʻoheheʻe e hoʻoheheʻe, e hoʻohui i 10 mL o ka sulfuric acid i hoʻoheheʻe ʻia, e hoʻoheheʻe me ka wai i ka nui, a hui.Ma mua koke o ka hoʻohana ʻana, e hoʻoheheʻe i 1.0 mL o kēia hopena me ka wai i 100.0 mL.
pH 6.0 acetate buffer: E hoʻoheheʻe i 50 g o ka ammonium acetate i 150 mL o ka wai, e hoʻoponopono me ka glacial acetic acid i kahi pH o 6.0, e hoʻoheheʻe me ka wai i 250 mL, a hui.
Hoʻonā maʻamau: E hoʻomākaukau i ka hui ʻana o 2.0 mL o ka solution alumini maʻamau, 10 mL o ka pH 6.0 acetate buffer, a me 98 mL o ka wai.E unuhi i kēia hui ʻana e like me ka wehewehe ʻana no ka hāpana Laʻana, e hoʻoheheʻe i nā ʻāpana i hui pū ʻia me ka chloroform i ka leo, a hui pū.
Laʻana hoʻonā: E hoʻoheheʻe i 20.0 g o Anhydrous Citric Acid i 100 mL o ka wai, a hoʻohui i 10 mL o ka pH 6.0 acetate buffer.Wehe i kēia hāpana me nā ʻāpana o 20, 20, a me 10 mL o kahi solution 0.5% o 8-hydroxyquinoline i loko o ka chloroform, e hoʻohui i nā ʻāpana chloroform i loko o kahi pahu volumetric 50-mL.E hoʻoheheʻe i nā mea i hui ʻia me ka chloroform i ka nui, a hui pū.
ʻO ka hopena hakahaka: E hoʻomākaukau i ka hui ʻana o 10 mL o ka pH 6.0 acetate buffer a me 100 mL o ka wai.E unuhi i kēia hui ʻana e like me ka wehewehe ʻana no ka Laʻana hoʻonā, hoʻoheheʻe i nā ʻāpana i hui pū ʻia me ka chloroform i ka nui, a hui pū.
Nā kūlana fluorometric
Ka lōʻihi o ka hawewe: 392 nm
Ka lōʻihi o ka hawewe: 518 nm
Kānāwai
Nā Laʻana: Haʻina maʻamau a me ka hāʻina Laʻana
E hoʻoholo i ka ikaika o ka fluorescence o nā Samples i kahi hoʻonohonoho fluorometer e like me ke kuhikuhi ʻana ma lalo o nā kūlana Fluorometric, me ka hoʻohana ʻana i ka solution Blank e hoʻonoho i ka mea hana i ka ʻole.
Nā pae hoʻohālikelike: ʻAʻole i ʻoi aku ka fluorescence o ka Sample solution ma mua o ka hopena maʻamau (0.2 ppm).
Nā mea haumia kino
• Ke Kaʻina Hana: Ka palena o ka ʻakika Oxalic
Laʻana waihona waihona: 200 mg/mL o Anhydrous Citric Acid i loko o ka wai
Haʻawina Laʻana: I ka 4 mL o ka Sample stock solution e hoʻohui i 3 mL o ka waikawa hydrochloric a me 1 g o ka zinc granular, e hoʻolapalapa no 1 min, a e kū no 2 min.E hoʻololi i ka supernatant i loko o kahi paipu hoʻāʻo i loaʻa ka 0.25 mL o kahi solution phenylhydrazine hydrochloride (1 i ka 100), a me ka wela i ka paila.E hoʻoʻalili me ka wikiwiki, e hoʻololi i ka cylinder i hoʻopaʻa ʻia, a hoʻohui i ka nui like o ka waika hydrochloric a me 0.25 mL o ka hopena ferricyanide potassium (1 i ka 20).E lulu, a e ku no 30 min.
Haʻina maʻamau: E hoʻomākaukau e like me ke kuhikuhi ʻia no ka Haʻina Haʻawina, koe wale nō ka hoʻohana ʻana i 4 mL o 0.10 mg/mL oxalic acid solution, e like me 0.0714 mg/mL o ka anhydrous oxalic acid, ma kahi o ka Sample stock solution.[E hoʻomaopopo—E hoʻomākaukau like me ka hāʻina Laʻana.]
Kānāwai
Nā Laʻana: Haʻina maʻamau a me ka hāʻina Laʻana
Nā pae ʻae ʻae: ʻAʻole ʻoi aku ka ikaika o kēlā me kēia kala ʻulaʻula i hana ʻia i loko o ka hāʻina Laʻana ma mua o ka mea i hana ʻia ma ka solution Standard (0.036%).
NA HOAO KUAI
• Ho'āʻo Endotoxins Bacterial 85: ʻO ke kiʻekiʻe o nā endotoxins bacteria e hiki ke hoʻokō ʻia ke koi i loko o ke ʻano o ke ʻano ʻano monograph (s) kahi i hoʻohana ʻia ai ka Anhydrous Citric Acid.Ma kahi e ʻōlelo ai ka lepili e pono e hoʻokau hou ʻia ka Anhydrous Citric Acid i ka wā o ka hoʻomākaukau ʻana i nā ʻano mea hoʻohana injectable, ʻo ke kiʻekiʻe o nā endotoxins bacteria e like me ke koi ʻana i ka monograph (s) pili i kahi i hoʻohana ʻia ai ka Anhydrous Citric Acid. halawai.
• Maopopo o ka Haʻina
[E hoʻomaopopo-E hoʻohālikelike ʻia ka hopena laʻana me ka hoʻokuʻu maʻamau A ma ke ao ākea 5 min ma hope o ka hoʻomākaukau ʻana o ka hoʻokuʻu maʻamau A. ]
ʻO Hydrazine sulfate solution: 10 mg/mL o ka hydrazine sulfate i loko o ka wai.E ʻae e kū no 4 a 6 mau hola ma mua o ka hoʻohana ʻana.
ʻO ka hopena Methenamine: E hoʻololi i 2.5 g o ka methenamine i loko o kahi pahu aniani 100-mL, hoʻohui i 25.0 mL o ka wai, hoʻokomo i ka pahu aniani, a hui a hoʻoheheʻe.
Hoʻokuʻu ʻia opalescent mua: E hoʻololi i 25.0 mL o ka solution Hydrazine sulfate i ka 25.0 mL o ka solution Methenamine i loko o ka ipu aniani i pani ʻia.E hui, a e ku no 24 h.[E hoʻomaopopo—Ua paʻa kēia hoʻokuʻu ʻia no 2 mahina, inā mālama ʻia ia i loko o kahi pahu aniani me ka ʻole o nā pōʻino.ʻAʻole pono ka hoʻokuʻu ʻana i ke aniani a pono e hui maikaʻi ʻia ma mua o ka hoʻohana ʻana.]
Opalescence maʻamau: Dilute 15.0 mL o ka Primary opalescent hoʻomaha me ka wai i 1000 mL.[Meka—ʻAʻole pono e hoʻohana ʻia kēia hoʻokuʻu ʻia ma mua o 24 mau hola ma hope o ka hoʻomākaukau ʻana.]
Hoʻokuʻu maʻamau A: E hoʻoheheʻe i 5.0 mL o Opalescence maʻamau me ka wai i 100 mL.
Hoʻokuʻu maʻamau B: E hoʻoheheʻe i 10.0 mL o Opalescence maʻamau me ka wai i 100 mL.
Laʻana hoʻonā: 200 mg/mL o Anhydrous Citric Acid i loko o ka wai
Kānāwai
Nā Laʻana: Hoʻokuʻu maʻamau A, Hoʻokuʻu maʻamau B, wai, a me ka hopena Laʻana
E hoʻololi i kahi ʻāpana kūpono o ka hopena laʻana i kahi paipu hoʻāʻo o ke aniani ʻeleʻele, aniani, kū ʻole me ke kumu pālahalaha a me ke anawaena o loko o 15-25 mm e loaʻa ai ka hohonu o 40 mm.E hoʻololi like i nā ʻāpana o ka hoʻokuʻu maʻamau A, ka hoʻokuʻu maʻamau B, a me ka wai e hoʻokaʻawale i nā paipu hoʻāʻo like.E hoʻohālikelike i ka hāʻina laʻana, ka hoʻokuʻu maʻamau A, ka hoʻokuʻu maʻamau B, a me ka wai i ke ao ākea, e nānā pono ana i kahi ʻeleʻele (e nānā i ka Spectrophotometry a me Light-Scattering 851, Visual Comparison).[E hoʻomanaʻo—Pono ka hoʻopuehu ʻana o ka mālamalama i hiki ke hoʻokaʻawale ʻia ka hoʻokuʻu maʻamau A me ka wai, a hiki ke hoʻokaʻawale ʻia ka hoʻokuʻu maʻamau B mai ka hoʻokuʻu maʻamau A. ]
Nā pae hoʻohālikelike: Hōʻike ka hāʻina laʻana i ka māmā like me ko ka wai.
• Ke kala o ka Haʻina
Haʻina waiwai maʻamau A: Ferric chloride CS, cobaltous chloride CS, a dilute hydrochloric acid (10 g/L) (2.4:0.6:7.0)
ʻO ka hoʻonā waiwai maʻamau B: Ferric chloride CS, cobaltous chloride CS, cupric sulfate CS, a me ka waika hydrochloric dilute (10 g/L) (2.4:1.0:0.4:6.2)
Haʻina waiwai maʻamau C: Ferric chloride CS, cobaltous chloride CS, a me cupric sulfate CS (9.6:0.2:0.2)
[E hoʻomanaʻo-E hoʻomākaukau i nā hoʻonā maʻamau ma mua o ka hoʻohana ʻana.]
Haʻina maʻamau A: E hoʻoheheʻe i 2.5 mL o ka solution stock maʻamau A me ka waika hydrochloric dilute (10 g/L) i 100 mL.
Haʻina maʻamau B: E hoʻoheheʻe i 2.5 mL o ka solution stock maʻamau B me ka waika hydrochloric dilute i (10 g/L) 100 mL.
Haʻina maʻamau C: E hoʻoheheʻe i 0.75 mL o ka wai hoʻoheheʻe maʻamau C me ka waika hydrochloric dilute (10 g/L) i 100 mL.
Laʻana Haʻina: E hoʻohana i ka Haʻina Haʻawina i hoʻomākaukau ʻia e like me ke kuhikuhi ʻana i ka hoʻāʻo no Clarity of Solution.
Kānāwai
Nā Laʻana: Haʻina maʻamau A, Haʻina maʻamau B, Haʻina maʻamau C, wai, a me ka hāʻina Laʻana
E hoʻololi i kahi ʻāpana kūpono o ka hopena laʻana i kahi paipu hoʻāʻo o ke aniani ʻeleʻele, aniani, kū ʻole me ke kumu pālahalaha a me ke anawaena o loko o 15-25 mm e loaʻa ai ka hohonu o 40 mm.E hoʻololi like i nā ʻāpana o ka solution maʻamau A, ka solution maʻamau B, ka solution maʻamau C, a me ka wai e hoʻokaʻawale i nā paipu hoʻolike like.Hoʻohālikelike i ka hāʻina laʻana, Haʻina maʻamau A, Haʻina maʻamau B, ʻŌnaehana maʻamau C, a me ka wai i ka lā ākea, e nānā pono ana i kahi kāʻei keʻokeʻo (e nānā i ka Spectrophotometry and Light-Scattering 851, Visual Comparison).
Nā pae ʻae ʻae: ʻAʻole ʻoi aku ka ʻoi o ka waihoʻoluʻu o ka Laʻana ma mua o ka solution maʻamau A, B, a i ʻole C, a i ʻole ka wai.
• Nā mea hiki ke maʻalahi
Laʻana: 1.0 g o ka pauka Anhydrous Citric Acid
Nānā: E hoʻololi i ka Laʻana i kahi paipu hoʻāʻo 22- × 175-mm i holoi mua ʻia me 10 mL o ka waikawa sulfuric a ʻae ʻia e kahe no 10 min.E hoʻohui i 10 mL o ka sulfuric acid, e hoʻoulu a hiki i ka pau ʻana o ka hopena, a e hoʻokomo i loko o kahi ʻauʻau wai ma 90 ± 1 no 60 ± 0.5 min, e mālama ana i ke kiʻekiʻe o ka waikawa ma lalo o ke kiʻekiʻe o ka wai i ka manawa holoʻokoʻa.E hooluolu i ka paipu i ka wai kahe, a e hoololi i ka waika i loko o ka paipu hoohalike kala.
Nā pae hoʻoholo: ʻAʻole ʻeleʻele ka waihoʻoluʻu o ka ʻakika ma mua o ka leo like o Matching Fluid K (e nānā i ke kala a me ka Achromicity 631) i loko o kahi paipu hoʻohālikelike, nānā ʻia nā paipu i ke kū pololei ma ke ʻano keʻokeʻo.
• Nā Ho'āʻo Sterility 71: Ma kahi e ʻōlelo ai ka lepili he sterile ka Anhydrous Citric Acid, hoʻokō ia i nā koi no ka Sterility Tests 71 ma ke ʻano monograph (s) pili i kahi e hoʻohana ʻia ai ka Anhydrous Citric Acid.
• Ka Hoʻoholo Wai, Ka Hana I 921: NMT 1.0%
NA KOI HOU
• Hoʻopili a me ka waiho ʻana: mālama i loko o nā pahu paʻa.ʻAʻole koi ʻia ka mālama ʻana.
• Lepili: Ma kahi i manaʻo ʻia no ka hoʻohana ʻana i nā hoʻonā dialysis, ua hoʻopaʻa inoa ʻia.Ma kahi o ka Anhydrous Citric Acid e pono e hoʻopili hou ʻia i ka wā o ka hoʻomākaukau ʻana i nā ʻano hana injectable e hōʻoia ai i nā pae kūpono o nā endotoxins bacteria, ua hōʻailona ʻia.Ma kahi o ka Anhydrous Citric Acid he sterile, ua kapa inoa ia.
• Nā Kūlana Kūlana USP 11
USP Citric Acid RS Kaomi e nānā i ke kūkulu
USP Endotoxin RS