D-(+)-Cycloserine CAS 68-41-7 Assay ≥ 900μg/mg Factory High Quality

Cycloserine [68-41-7].
He mana ko Cycloserine ma lalo o 900µg o C3H6N2O2 no ka mg.
Hoʻopili a mālama ʻia - mālama i loko o nā pahu paʻa.
Nā kūlana hōʻike USP <11>-
USP Cycloserine RS
Identification-Dissolve ma kahi o 1 mg i 10 mL o 0.1 N sodium hydroxide.I ka 1 mL o ka hopena hopena e hoʻohui i 3 mL o 1 N acetic acid a me 1 mL o kahi hui ʻana, i hoʻomākaukau ʻia i 1 hola ma mua o ka hoʻohana ʻana, o nā ʻāpana like o ka solution sodium nitroprusside (1 i ka 25) a me 4 N sodium hydroxide: he kala polū mālie. ulu ana.
Nā huahana condensation-ʻO kona absorptivity (e nānā i ka Spectrophotometry and Light-Scattering <851>) ma 285 nm, i hoʻoholo ʻia i loko o kahi solution 0.1 N sodium hydroxide i loaʻa ka 0.40 mg no ka mL ʻaʻole i oi aku ma mua o 0.80.
Ka hoʻololi kikoʻī <781S>: ma waena o 108° a me 114°.ʻO ka hopena hoʻāʻo: 50 mg no ka mL, i ka 2 N sodium hydroxide.
Crystallinity <695>: kūpono i nā koi
pH <791>: ma waena o 5.5 a me 6.5, ma kahi hoʻonā (1 i ka 10).
Nalo ma ka maloʻo <731>-Hoʻomaloʻo ma kahi o 100 mg i loko o ka ʻōmole capillary y-stoppered i loko o ka vacuum ma 60 ℃ no 3 mau hola: ʻaʻole e nalowale ma mua o 1.0% o kona kaumaha.
ʻO ke koena ma ka hoʻā ʻana <281>: ʻaʻole i ʻoi aku ma mua o 0.5%, hoʻomaʻemaʻe ʻia ke koena i hoʻopau ʻia me 2 mL o ka waikawa nitric a me 5 kulu o ka waikawa sulfuric.
hoʻāʻo-
pH 6.8 Phosphate buffer-E hoʻomākaukau e like me ke kuhikuhi ʻana i ka Buffer Solutions ma lalo o Solutions ma ka ʻāpana Reagents, Indicators, and Solutions.
Mobile phase-Dissolve 0.5 g o ka sodium 1-decanesulfonate i 800 mL o ka wai, e hoʻohui i 50 mL o ka acetonitrile a me 5 mL o ka waikawa acetic glacial, a hui.Hoʻoponopono me 1 N sodium hydroxide i kahi pH o 4.4.Kānana, a degas.Hana i nā hoʻololi inā pono y (e ʻike i ka System Suitability ma lalo o Chromatography <621>).
Hoʻomākaukau maʻamau-E hoʻoheheʻe i ka nui o ka USP Cycloserine RS i ka pH 6.8 Phosphate buffer no ka loaʻa ʻana o kahi hopena i ʻike ʻia ma kahi o 0.4 mg no ka mL.
Hoʻomākaukau hoʻāʻo-E hoʻoneʻe e pili ana i 20 mg o Cycloserine, i kaupaona pololei ʻia, i kahi pahu volumetric 50-mL, hoʻoheheʻe i loko a hoʻoheheʻe me ka pH 6.8 Phosphate buffer i ka leo, a hui.
Pūnaehana Chromatographic (e nānā i ka Chromatography <621>)-Ua hoʻolako ʻia ka chromatograph wai me kahi mea ʻike 219-nm a me kahi kolamu 4.6-mm × 25-cm i loaʻa iā 5- µm packing L1.ʻO ka nui o ke kahe e pili ana i 1 mL i kēlā me kēia minuke.Mālama ʻia ka mahana o ke kolamu ma kahi o 30 °.Chromatograph i ka hoʻomākaukau maʻamau, a hoʻopaʻa i nā pane kiʻekiʻe e like me ke kuhikuhi ʻana no ke Kaʻina Hana: ʻaʻole ʻoi aku ka nui o ka huelo ma mua o 1.8;a ʻo ka ʻokoʻa maʻamau pili no ka hoʻopiʻi hou ʻana ʻaʻole ia ma mua o 2.0%.
Kaʻina hana-Hoʻokaʻawale i nā puke like (ma kahi o 10 µL) o ka hoʻomākaukau maʻamau a me ka hoʻomākaukau ʻana i ka Assay i loko o ka chromatograph, e hoʻopaʻa i nā chromatograms, a ana i nā pane kiʻekiʻe no ka cycloserine.E helu i ka nui, ma µg, o C3H6N2O2 i kēlā me kēia mg o Cycloserine i lawe ʻia e ke kumu:
50,000(C/W)(rU / rS)
i loko o ka C ka manaʻo, i ka mg per mL, o USP Cycloserine RS i ka hoʻomākaukau maʻamau;ʻO W ka nui, ma mg, o Cycloserine i lawe ʻia no ka hoʻomākaukau ʻana i ka hoʻomākaukau ʻana;a ʻo rU a me rS nā pane kiʻekiʻe no ka cycloserine i loaʻa mai ka hoʻomākaukau Assay a me ka hoʻomākaukau maʻamau.