D-Histidine CAS 351-50-8 HD-His-OH Assay 98.5~101.0% Hale Hana

pH
e lawe i 1.0g o kēia huahana, hoʻohui i 50ml o ka wai e hoʻoheheʻe, a ana e like me ke kānāwai (General rule 0631).Pono ka waiwai o ka pH 7.0-8.5.
Ka hoʻouna ʻana o ka hoʻonā
E lawe i ka 0.60g o kēia huahana, e hoʻohui i ka wai 20ml e hoʻoheheʻe, e like me ka UV-visible spectrophotometry (General rule 0401), e ana i ka transmittance ma 430nm wavelength, ʻaʻole i emi iho ma mua o 98.0%.
Klorida
E lawe i 0.25g o kēia huahana a nānā e like me ke kānāwai (General rule 0801).Ke hoʻohālikelike ʻia me ka hopena hoʻomalu i hana ʻia me 0.02% o ka solution sodium chloride maʻamau, ʻaʻole pono e hoʻonui ʻia ().
Sulfate
E lawe i 1.0g o kēia huahana a nānā e like me ke kānāwai (General rule 0802).Ke hoʻohālikelike ʻia me ka hoʻonā mana i hana ʻia i ka 0.02% o ka hopena potassium sulfate maʻamau, ʻaʻole pono e ʻoi aku ka nui ().
Paakai Ammonium
e lawe i 0.10g o kēia huahana a nānā e like me ke kānāwai (General rule 0808).Ke hoʻohālikelike ʻia me ka hoʻonā mana i hana ʻia me 0.02% o ka solution ammonium chloride maʻamau, ʻaʻole ia e hohonu ().
Nā ʻAmino ʻē aʻe
e lawe i ka nui kūpono o kēia huahana, e hoʻohui i ka wai e hoʻoheheʻe a hoʻoheheʻe ʻia e hana i kahi hopena i loaʻa ma kahi o 10 mg no ka lml ma ke ʻano he hopena hoʻāʻo;E lawe i 1ml no ke ana pololei a hookomo i loko o ka ipu ana he 200ml, e hoohehee i ka unahi me ka wai, e lulu maikai, i mea hooponopono;E lawe i ka nui kūpono o ka histidine reference a me ka proline reference, a waiho i loko o ka ipu ana like, ua hoʻohui ʻia ka wai e hoʻoheheʻe a hoʻoheheʻe ʻia no ka hoʻomākaukau ʻana i kahi hopena i loaʻa ma kahi o 0.4mg kēlā me kēia 1ml ma ke ʻano he ʻōnaehana kūpono.E like me ka ho'āʻo chromatography papa lahilahi (General 0502), e kaha kiʻi i 5 u1 o kēlā me kēia o nā haʻina ʻekolu ma luna, e kuhikuhi ma luna o ka silica gel G ʻili lahilahi pā, me ka N-propanol-concentrated ammonia solution (67:33) e like me ka ka hoʻomohala ʻana i ka mea hoʻoheheʻe, hoʻonui, hoʻomaloʻo, kāpī ʻia me ka ninhydrin i loko o ka acetone solution (1-50), wela ma 80 ° C a hiki i ka ʻike ʻana o nā kiko, a nānā koke.Pono e hōʻike i ka ʻaha hoʻomalu i kahi kikoʻī, a ʻo ka ʻōnaehana pili pono e hōʻike i ʻelua mau wahi kaʻawale loa.Inā hōʻike ka hopena hoʻāʻo i nā wahi haumia, ʻaʻole pono ka hohonu o ke kala (0.5%) ma mua o ka wahi nui o ka hoʻonā mana.
Nalo ma ka maloo
E lawe i kēia huahana, maloʻo ma 105 ℃ no 3 mau hola, ʻaʻole e oi aku ka pohō o ke kaumaha ma mua o 0.2% (General rule 0831).
Koena ma ka Ignition
ʻAʻole i ʻoi aku ma mua o 0.1% (Ke kānāwai nui 0841).
Paakai Hao
e lawe i 1.0g o kēia huahana a nānā e like me ke kānāwai (General rule 0807).Ke hoʻohālikelike ʻia me ka hoʻonā mana i hana ʻia me 0.001% o ka hoʻonā hao maʻamau, ʻaʻole ia e hohonu ().
Nā metala kaumaha
ʻO kēia huahana 1.0g, nānā e like me ke kānāwai (General Principles 0821 The first law), ʻaʻole i ʻoi aku ka nui o nā metala kaumaha ma mua o 10 mau ʻāpana o ka miliona.
Paakai Arsenic
e lawe i 2.0g o kēia huahana, e hoʻohui i 23ml wai e hoʻoheheʻe, e hoʻohui i 5ml o ka waika hydrochloric, e nānā e like me ke kānāwai (General rule 0822 mua kānāwai), pono e hoʻokō me nā mea i hoʻolako (0.0001%).
Endotoxin maʻi bacteria
E lawe i kēia huahana, e nānā e like me ke kānāwai (General rule 1143), ʻo kēlā me kēia lg histidine i loaʻa ka endotoxin e emi iho ma mua o 6.0EU.(No ka injection)
351-50-8 - Hoʻoholo maʻiʻo
E lawe i kēia huahana ma kahi o 0.15g, pololei ke kaupaona ʻana, e hoʻohui i ka anhydrous formic acid 2ml e hoʻoheheʻe, e hoʻohui i ka glacial acetic acid 50ml, e like me ke ʻano titration hiki (General rule 0701), me ka perchloric acid titration solution (0.1 mol/L) titration, a ua hoʻoponopono ʻia nā hopena o ka titration me kahi hoʻāʻo blank.ʻO kēlā me kēia 1 ml perchloric acid titration solution (0.1 mol/L) pili i 15.52mg o C6H9N302.