D-Leucine CAS 328-38-1 HD-Leu-OH Assay 98.5~101.5% Factory High Quality

pH
E lawe i 0.50g o kēia huahana, hoʻohui i 50ml o ka wai, wela e hoʻoheheʻe, anuanu, a hoʻoholo e like me ke kānāwai (General 0631), pono ka pH 5.5 ~ 6.5.
Ka hoʻouna ʻana o ka hoʻonā
E lawe i ka 0.50g o kēia huahana, hoʻohui i 50ml o ka wai, wela e hoʻoheheʻe, ʻoluʻolu, ultraviolet-visible spectrophotometry (General rule 0401), e ana i ka transmittance ma 430nm wavelength, ʻaʻole i emi iho ma mua o 98.0%.
Klorida
E lawe i 0.25g o kēia huahana a nānā e like me ke kānāwai (General rule 0801).Ke hoʻohālikelike ʻia me ka hoʻonā mana i hana ʻia me 5.0 ml o ka solution sodium chloride maʻamau, ʻaʻole pono e ʻoi aku ka nui (0.02%).
Sulfate
E lawe i 1.0g o kēia huahana a nānā e like me ke kānāwai (General rule 0802).Ke hoʻohālikelike ʻia me ka hoʻonā mana i hana ʻia o 2.0 ml o ka hopena potassium sulfate maʻamau, ʻaʻole pono e hoʻonui ʻia (0.02%).
Paakai Ammonium
E lawe i kēia huahana 0.10g, e nānā e like me ke kānāwai (General rule 0808), a hoʻohālikelike me ka hoʻonā mana i hana ʻia me ka solution ammonium chloride maʻamau 2.0 ML, ʻaʻole hohonu (0.02%).
ʻO ka waikawa amino ʻē aʻe
E lawe i ka nui kūpono o kēia huahana, e hoʻohui i ka wai e hoʻoheheʻe a hoʻoheheʻe ʻia e hana i kahi hopena i loaʻa ma kahi o 20mg no ka lml ma ke ʻano he hopena hoʻāʻo;E lawe i 1ml no ke ana pololei a waiho i loko o ka ipu ana 200ml, e hoʻoheheʻe i ka paona me ka wai, e lulu, ma ke ʻano he hoʻonā mana;E lawe i ka nui kūpono o ka leucine reference a me ka valine, a hoʻokomo i loko o ka pahu ana hoʻokahi, ua hoʻohui ʻia ka wai e hoʻoheheʻe a hoʻoheheʻe ʻia e hoʻomākaukau i kahi hopena i loaʻa ma kahi o 0.4mg kēlā me kēia 1ml ma ke ʻano he ʻōnaehana kūpono.E like me ka hoʻāʻo ʻana o ka chromatography papa lahilahi (General 0502), e hoʻomoʻa i nā haʻina ʻekolu ma luna o kēlā me kēia 5 u1, i kēlā me kēia, ma ka silica gel G ʻili lahilahi pā, me ka n-butanol-wai-glacial acetic acid (3:1:1) no ka hoʻomohala ʻana, ma hope o ka waiho ʻana, e hoʻomaloʻo i ka ea, e kāpīpī me ka ninhydrin i loko o ka acetone solution (1-50), e hoʻomehana ma 80 ° C a hiki i ka ʻike ʻana o nā kiko, a me ka nānā koke ʻana.Pono e hōʻike i ka ʻaha hoʻomalu i kahi kikoʻī, a ʻo ka ʻōnaehana pili pono e hōʻike i ʻelua mau wahi kaʻawale loa.Inā hōʻike ka hopena hoʻāʻo i nā wahi haumia, ʻaʻole pono ka hohonu o ke kala (0.5%) ma mua o ka wahi nui o ka hoʻonā mana.
Nalo ma ka maloo
E lawe i kēia huahana, maloʻo ma 105 ℃ no 3 mau hola, ʻaʻole e oi aku ke kaumaha o ke kaumaha ma mua o 0.2% (Ke kānāwai nui 0831).
Koena ma ka Ignition
E lawe i 1.0g o kēia huahana a nānā e like me ke kānāwai (General rule 0841).ʻAʻole ʻoi aku ke koena o ke koena ma mua o 0.1%.
Paakai Hao
E lawe i 1.5g o kēia huahana a nānā e like me ke kānāwai (General rule 0807).Ke hoʻohālikelike ʻia me ka hoʻonā mana i hana ʻia me 0.001% o ka hoʻonā hao maʻamau, ʻaʻole ia e hohonu ().
Metala Kaumaha
ʻO ke koena i waiho ʻia ma lalo o ke ʻano o ka lawe ʻana i ke koena hoʻā ahi ʻaʻole e loaʻa ma mua o 10 mau ʻāpana o ka miliona o ka metala kaumaha ke nānā ʻia e ke kānāwai (General Principles 0821, Law II).
Paakai Arsenic
E hoʻohui i ka wai 5ml, 1ml sulfuric acid a me 10ml sulfurous acid, wela i ka nui o kahi 2ml i loko o ka wai ʻauʻau, hoʻohui i ka wai 5ml, hoʻohui i ka solution Dropwise ammonia a hiki i ka mea hōʻailona o ka phenolphthalein e kū ʻole, hoʻohui i ka waika hydrochloric 5ml, hoʻohui i ka wai e hana i 28ml , e like me ka nānā ʻana i ke kānāwai (General Principles 0822 The first law), pono e hoʻokō i nā ʻōlelo (0.0001%).
Endotoxin maʻi bacteria
E lawe i kēia huahana, e nānā e like me ke kānāwai (General 1143), ʻo kēlā me kēia lg leucine i loaʻa ka endotoxin e emi iho ma mua o 25EU.(No ka injection)
328-38-1 - Ka Manaʻo Hoʻoholo
E lawe i kēia huahana e pili ana i 0.1g, pololei ke kaupaona ʻana, e hoʻohui i ka anhydrous formic acid 1 ml i hoʻoheheʻe ʻia, e hoʻohui i ka ʻakika acetic glacial 25ml, e like me ke ʻano potentiometric titration (General rule 0701), me ka perchloric acid titration solution (O. 1 mol/L) titration , a ua hoʻoponopono ʻia nā hopena o ka titration me kahi hoʻāʻo blank.ʻO kēlā me kēia 1 ml o ka waika perchloric titration solution (0.1 mol/L) pili i 13.12 mg o C6H13N02.