D-Tryptophan CAS 153-94-6 (HD-Trp-OH) Hōʻike 98.5~101.0% Hale Hana

ʻakika
E lawe i 1g o kēia huahana, hoʻohui i 50ml wai e hoʻoheheʻe, a ana e like me ke kānāwai (General Rule 5.4).Pono ka pH waiwai he 5.4-6.4.
Ka lawe ʻana o ka hoʻonā
e lawe i 0.5g o kēia huahana, e hoʻohui i ka 2mol/L hydrochloric acid solution 20ml e hoʻoheheʻe, e like me ka ultraviolet-visible spectrophotometry (General rule 0401), e ana i ka transmittance ma 430nm wavelength, ʻaʻole i emi iho ma mua o 95.0%.
Klorida
E lawe i 0.25g o kēia huahana a nānā e like me ke kānāwai (General rule 0801).Ke hoʻohālikelike ʻia me ka hopena hoʻomalu i hana ʻia me 0.02% o ka solution sodium chloride maʻamau, ʻaʻole pono e hoʻonui ʻia ().
Sulfate
E lawe i ka l.0g o kēia huahana a nānā e like me ke kānāwai (General rule 0802).Ke hoʻohālikelike ʻia me ka hoʻonā mana i hana ʻia i ka 0.02% o ka hopena potassium sulfate maʻamau, ʻaʻole pono e ʻoi aku ka nui ().
Paakai Ammonium
E lawe i 0.10g o kēia huahana a nānā e like me ke kānāwai (General rule 0808).Ke hoʻohālikelike ʻia me ka hoʻonā mana i hana ʻia me 0.02% o ka solution ammonium chloride maʻamau, ʻaʻole ia e hohonu ().
Nā ʻAmino ʻē aʻe
E lawe i ka 0.30g o kēia huahana, e hoʻokomo i loko o kahi pahu ana 20ml, e hoʻohui i ka 1 mol / L hydrochloric acid solution 1 ml a me ka wai e hoʻoheheʻe, e hoʻoheheʻe me ka wai i ka unahi, e lulu maikaʻi, e like me ka hopena ho'āʻo;e lawe pono i ka 1 ml, e hookomo i loko o ka ipu ana 200ml, e hoohehee me ka wai, e lulu maikai, a e hoohana ia mea ma ke ano he hooponopono.E lawe i ka 10mg o ka tryptophan control a me ka tyrosine control, a hookomo i loko o ka ipu ana 25ml like, 1 ml o 1 mol/L hydrochloric acid solution a me ka nui o ka wai i hoohuiia no ka hoopau ana.Ua hoʻoheheʻe ʻia ka hopena i ka paona me ka wai a laila hoʻoluliluli ʻia e hana i kahi hopena kūpono no ka ʻōnaehana.E like me ka ho'āʻo chromatography papa lahilahi (General 0502), e hoʻomohu i nā haʻina ʻekolu ma luna o kēlā me kēia 2 μL, i kēlā me kēia, ma ka silica gel G ʻili lahilahi pā, me ka n-butanol-glacial acetic acid-wai (3:1:1) e hohola, e hohola, e maloo, e pulupulu me ka ninhydrin i ka acetone solution (1-50), e wela ma 80 ℃ a hiki i ka ikea ana o ka * kiko, a nana koke.Pono e hōʻike i ka ʻaha hoʻomalu i kahi kikoʻī, a ʻo ka ʻōnaehana pili pono e hōʻike i ʻelua mau wahi kaʻawale loa.Inā hōʻike ka hopena hoʻāʻo i nā wahi haumia, ʻaʻole pono ka hohonu o ke kala (0.5%) ma mua o ka wahi nui o ka hoʻonā mana.
Nalo ma ka maloo
E lawe i kēia huahana, maloʻo ma 105 ℃ no 3 mau hola, ʻaʻole e oi aku ke kaumaha o ke kaumaha ma mua o 0.2% (Ke kānāwai nui 0831).
Koena ma ka Ignition
E lawe i 1.0g o kēia huahana a nānā e like me ke kānāwai (General rule 0841).ʻAʻole ʻoi aku ke koena o ke koena ma mua o 0.1%.
Paakai Hao
E lawe i kēia huahana 1.0g ma hope o ka puhi ʻana a me ka lehu, e hoʻohui i ka waika hydrochloric (2ml) i ke koena, e hoʻomaloʻo i ka wai ʻauʻau, a laila e hoʻohui i ka waika hydrochloric dilute (4ml), e hoʻoheheʻe i ka wela iki, e hoʻohui i ka wai (30ml) a me ka ammonium persulfate (50mg), a nānā iā ia e like me ke kānāwai (General rule 0807), ʻaʻole pono e hohonu (0.002%) ma mua o ka hoʻonā mana i hana ʻia i ka hopena hao maʻamau.
Metala Kaumaha
ʻO ke koena i waiho ʻia ma lalo o ke ʻano o ka lawe ʻana i ke koena hoʻā ahi ʻaʻole e loaʻa ma mua o 10 mau ʻāpana o ka miliona o ka metala kaumaha ke nānā ʻia e ke kānāwai (General Principles 0821, Law II).
Paakai Arsenic
e lawe i ka 2.0g o kēia huahana, e hoʻohui i 5ml o ka waika hydrochloric a me 23ml o ka wai e hoʻoheheʻe, e nānā e like me ke kānāwai (General rule 0822 mua kānāwai), pono e hoʻokō me nā mea (0.0001%).
Endotoxin maʻi bacteria
E lawe i kēia huahana, e hoʻohui i ka wai hoʻāʻo endotoxin kanaka, a me ka wela i 80 ℃ e hoʻoheheʻe ʻia, e nānā e like me ke kānāwai (General 1143), ʻo kēlā me kēia lg o tryptophan i loaʻa ka endotoxin e emi iho ma mua o 50EU.(No ka injection)
Hoʻoholo maʻiʻo
E lawe i kēia huahana ma kahi o 0.15g, pololei ke kaupaona ʻana, e hoʻohui i ka anhydrous formic acid 3ml i hoʻoheheʻe ʻia, e hoʻohui i ka glacial acetic acid 50ml, e like me ke ʻano titration hiki (General rule 0701), me ka hydrochloric acid titration solution (0.1 mol/L) titration, a me ka Ua hoʻoponopono ʻia nā hopena o ka titration me kahi hoʻāʻo blank.ʻO kēlā me kēia 1 ml o ka waika perchloric titration solution (0.1 mol/L) pili i 20.42mg o C11H12N2O2.