EDC·HCl CAS 25952-53-8 Coupling Reagent Purity >99.0% (T) Factory

ʻO 1-(3-Dimethylaminopropyl) -3-Ethylcarbodiimide Hydrochloride (EDC·HCl) (CAS: 25952-53-8) he pauka crystalline keʻokeʻo, maʻalahi deliquescent, hiki ke hoʻoheheʻe ʻia i ka wai, hiki ke hoʻoheheʻe ʻia i ka ethanol, hoʻohana ʻia ma ke ʻano he reagent hoʻāla no ka carboxyl. nā pūʻulu i ka amide synthesis, hoʻohana pū ʻia no ka hoʻoulu ʻana i nā pūʻulu phosphate, ka hoʻopili ʻana i nā protein a me nā waikawa nucleic, a me ka hana ʻana i nā immunocoupler.Hoʻohana ʻia ʻo EDC-HCl i ka pae pH o 4.0-6.0 a hoʻohana pinepine ʻia me ka N-Hydroxysuccinimide (NHS) a i ʻole N-Hydroxysulfosuccinimide sodium paʻakai e hoʻomaikaʻi i ka pono hoʻohui.
ʻO EDC·HCl he carbodiimide hiki ke hoʻoheheʻe ʻia i ka wai, hoʻohana nui ʻia no ka hui ʻana o ka peptide.Hoʻohana ʻia ʻo EDC·HCl ma ke ʻano he carboxyl activating agent.He hana koʻikoʻi ia no ka immobilization o nā biomolecules nui i hui pū me N-hydroxysuccinimide.Hoʻohana ʻia ia i ka acylation o phosphoranes.
ʻO ka wai-soluble derivative o carbodiimide pono no ka hoʻohui ʻana i nā haptens i nā protein a me nā polypeptides.Hoʻohana ʻia e hoʻololi i nā mea loaʻa NMDA a ma ke ʻano he condensing agent i ka peptide synthesis.ʻO ka pōmaikaʻi nui o ka hui ʻana o EDAC ʻo ia ka maʻalahi o ka wehe ʻana i ka reagent keu a me ka urea e like me ka holoi ʻana me ka waika wai a i ʻole ka wai.Hoʻopili ʻo Carbodiimides i ka hoʻokumu ʻana i nā paʻa amide, nā ʻakika carboxylic, a me nā amine ma ka hoʻoulu ʻana i ka carboxylate e hana i kahi O-acylurea.Hiki ke hoʻouka ʻia kēia kikowaena e kahi amine e hana i kahi amide.Hoʻokuʻu ʻia ʻo EDAC ma ke ʻano he urea derivative.
Hiki ke hoʻohana ʻia ʻo EDC·HCl no ka hana ʻana i nā amides.Hoʻohana ʻia ia ma ke ʻano he mea hoʻohui i ka synthesis o nā esters mai nā waikawa carboxylic e hoʻohana ana i ka dimethylaminopyridine e like me ka catalyst.
ʻEkolu mau mea hana condensing maʻamau: DCC, DIC a me EDC·HCl.ʻO ka hoʻohana ʻana i kēia ʻano condensing agent maʻamau e koi i ka hoʻohui ʻana i nā catalysts acylation a i ʻole activators, e like me DMAP, HOBt, etc. ʻAʻole hoʻohana ʻia ka catalyst e hoʻohuli iā ia i loko o ka ester ikaika a i ʻole ka amide hana, e hoʻonohonoho hou ʻia i loko o ka huahana paʻa kūpono o ka urea (Ala B).Mea hoʻoulu condensation: Aia nā ʻano o ka mea hoʻoulu condensation maʻamau.I kēia manawa, ua hoʻohana nui ʻia ka 4-N,N-dimethylpyridine (DMAP) no ka hoʻoulu ʻana i nā hopena acylation.I kekahi manawa hiki ke hoʻohana ʻia ka 4-PPY inā ʻaʻole maikaʻi ka hopena catalytic o DMAP.Wahi a nā palapala pili, ʻo kona hiki ke catalytic ma kahi o hoʻokahi tausani mau manawa kiʻekiʻe ma mua o DMAP.ma waena o nā condensers maʻamau ʻekolu, ʻoi aku ka liʻiliʻi o DCC a me DIC.Hoʻohana pū ʻia ʻo DCC a me DMAP.ʻO kekahi o nā pōʻino nui loa o ka hoʻohana ʻana i ka DCC ʻo ia ka huahana ʻē aʻe o ka hopena, dicyclohexylurea, he liʻiliʻi ka solubility i loko o ka māhele organik maʻamau akā hiki ke hoʻoheheʻe iki, no laila paʻakikī ke wehe pono ʻia ma o kekahi mau hana hoʻomaʻemaʻe maʻamau, recrystallization, kolamu chromatography, etc.;no ka mea, he liʻiliʻi ka solubility o dicyclohexylurea i loko o ka etera ma mua o nā mea hoʻoheheʻe ʻē aʻe, no laila, ʻo ka mālama ʻana i kēia ʻano hopena e hoʻoheheʻe maʻamau i ka solvent hopena a hoʻohui i ka etera, a kānana i ka hapa nui o ka dicyclohexylurea ma mua o ka mālama hou ʻana.No ka mea, ʻoi aku ka maikaʻi o ka solubility o ka diisopropylurea i hana ʻia e DIC i nā mea hoʻoheheʻe organik maʻamau, hoʻohana maʻamau ia i ka hoʻohui ʻana i nā kemika solid-phase synthesis.Hoʻohana nui ʻia ʻo EDCI i kēia manawa i ka lāʻau kemika.ʻO kekahi o kāna mau hiʻohiʻona nui, ʻo ia ka urea i hana ʻia ma hope o ka hopena o ka wai a hiki ke holoi ʻia.ʻO ka mea maʻamau, ua hui pū ʻia ʻo EDCI me HOBt (nota: ʻo kēia hopena HOBt he mea nui loa ia, inā ʻaʻole hiki ke alakaʻi i ka hopena condensation haʻahaʻa loa).I kekahi manawa inā nui ka ʻākeʻe o ka ʻakika a i ʻole he pūʻulu hoʻihoʻi electron, e mau ana ka hopena i ka ʻanuʻu ester hana (he hōʻailona kikoʻī nui ko ka ester ikaika a hiki ke ʻike ʻia e MS a i ʻole LC-MS).