Fmoc-Asp(OtBu)-OH CAS 71989-14-5 Maʻemaʻe >99.0% (HPLC) Hale Hana

ʻO Fmoc-L-Aspartic Acid β-tert-Butyl Ester (Fmoc-Asp(OtBu) -OH) (CAS: 71989-14-5) he mea waena kemika, hoʻohana maʻamau i ka hana polypeptide synthesis, no ka pale o ka waikawa amino.ʻO Fmoc-amino acids, hoʻohana ʻia i ka peptide synthesis, hoʻohana ʻia ma ke ʻano he mea hoʻohuihui kūlohelohe, waena lāʻau lapaʻau, biochemical reagent a i ʻole reagent kemika.
Hoʻomākaukau: E hoʻohui i 581g o ka tert-Butyl Acetate a me 133g o ka Aspartic Acid i kahi ML ʻekolu waha omole, hoʻoulu, a hoʻohui i ka perchloric acid dropwise, pane i ka 10 ℃ no 48 mau hola, hoʻomaha i 0 ℃, a laila hoʻohui i 700ml wai, i unuhiʻia (ke koena tert-Butyl Acetate e like me ka solvent extraction), neutralized me Na2CO3 i ka pH = 7, ka hoʻokaʻawale ʻana o ka wai, ua hoʻopaʻa ʻia ka papa tert-Butyl Acetate ma lalo o ke kaomi hoʻemi e loaʻa ai ka hui ʻana o Asp(OtBu)2, Asp(OtBu) a me ka aila Asp-OtBu, i huiia me ka papa wai e hoohanaia.Ua hoʻoili ʻia ka hui ʻana i ka 125 ml ʻekolu mau hue, ua hoʻohui ʻia ʻo G cuso4 · 5H2O, a ua hoʻoponopono ʻia ka pH i 3 me ka waikawa hydrochloric concentrated.Hoʻoulu ʻia ka hui ʻana a hoʻomehana ʻia i 50 ℃.No 12 hola e hoʻomākaukau ai iā Cu[Asp(OtBu)]x.(x = 1~2);A laila i lalo i ka lumi wela, e hoʻohui i ka 116.2G tetramethylethylenediamine a me 300ml dioxane, a laila hoʻoponopono i ka pH i 8 ~ 9 me ka triethylamine e hoʻomākaukau ai iā Asp(OtBu);a laila e hoʻohui i ka 82gFmoc-OSu i Asp(OtBu), mālama i ka pH 8 ~ 9, e hana no nā hola 8, a laila e unuhi me ka ethyl acetate, acidify, holoi me ka wai, ka hoʻoheheʻe ʻana i ka wai, crystallization, maloʻo, 85g o Fmoc-L-aspartic acid beta. Ua loaʻa ka -tert-butyl ester ma kahi hua o 20.7%.Ma o ka hoʻopili HPLC o Fmoc-L-Aspartic Acid β-tert-Butyl Ester.