Fmoc-Gly-OH CAS 29022-11-5 Fmoc-Glycine Maʻemaʻe >99.0% (HPLC) Hale Hana

ʻO Fmoc-Glycine (Fmoc-Gly-OH) (CAS: 29022-11-5) kahi ʻano pale N-Fmoc o Glycine.ʻO Glycine kahi waikawa amino pono ʻole.ʻO Fmoc-amino acids, hoʻohana ʻia i ka peptide synthesis, hoʻohana ʻia ma ke ʻano he mea hoʻohuihui kūlohelohe, waena lāʻau lapaʻau, biochemical reagent a i ʻole reagent kemika.
Introduction: ʻO Fmoc-Glycine ka Glycine i pale ʻia e Fmoc.ʻO ka Fluorene methoxycarbonyl (Fmoc) he pūʻulu pale e pili ana i ke kumu, hiki ke hoʻohana ʻia i ka wai ammonia concentrated a i ʻole dioxane-methanol-4N NaOH (30: 9: 1) a me ka piperidine, ethanolamine, cyclohexylamine, 1,4-Dioxane, pyrrolidone a me ʻē aʻe ammonia 50% dichloromethane solution.Ma lalo o nā kūlana alkaline nāwaliwali e like me ka sodium carbonate a i ʻole ka sodium bicarbonate, hoʻokomo pinepine ʻia ka hui pale Fmoc e Fmoc-Cl a i ʻole Fmoc-OSu.
Noi: ʻO Fmoc-Glycine ka Fmoc-protected Glycine, ka mea i hoʻohana nui ʻia e synthesize polypeptides.ʻO ka ʻokoʻa o nā pūʻulu pale amino acid he hopena nui i ka pono a me ka hua o ka polypeptide synthesis.No ka laʻana, hiki ke hoʻohana ʻia ʻo Fmoc-Glycine e hoʻomākaukau ai i kahi mea hoʻopaneʻe glycine derivative corrosion, a ʻo ka hui pale Fmoc a me ke kaulahao lōʻihi hydrophobic e pili ana i ka mole Glycine ma o ka hopena amidation, a me ka heteroatom (N, O, etc.) A i ʻole. unsaturated paʻa) a me ka bisphenyl apo i loaʻa π electrons ua adsorbed ma luna o ka metala, a me ka hydrophobic kaulahao lōʻihi poloka i ka wai molekele, i mea e hana i ka pale kiʻiʻoniʻoni ma luna o ke kila kila e hoʻokō i ke kumu o ka corrosion pale.
Hoʻomākaukau: Fmoc-Glycine synthesis nā ʻanuʻu kikoʻī penei: 3.0g Glycine i hoʻoheheʻe ʻia i 115ml o 10% Na2CO3, hoʻoluʻu i -4 ~ 0 ℃, 11.2g Fmoc-Cl i hoʻoheheʻe ʻia i 35.0ml o ka acetone a hoʻohui ʻia i ka ʻōnaehana dropwise, ma hope o ka hoʻohui dropwise, e hoʻoulu i ka hau hau no 30min, a laila e hoʻoulu i ka lumi wela no 2.0h.Ua nānā ʻia ka holomua ʻana e ka chromatography layer thin.Ma hope o ka pau ʻana o ka hopena, ua ninini ʻia ka 400ml o ka wai i loko o ka wai, ua unuhi ʻia ka ether i ʻelua manawa, hoʻomaʻamaʻa a hoʻoponopono ʻia i ka pH2 me ka waikawa hydrochloric concentrated, ua hoʻoheheʻe ʻia ka nui o ke keʻokeʻo keʻokeʻo, 50 ml o ka ethyl acetate i unuhi ʻia ʻekolu mau manawa, anhydrous magnesium. Ua hoʻomaloʻo ʻia ka sulfate, ua hoʻoheheʻe ʻia ka mea hoʻoheheʻe ma lalo o ke kaomi hoʻemi ʻia, a ua hoʻohana ʻia ka petroleum etera e hoʻoheheʻe ʻia e loaʻa ai ka 11.5g o Fmoc-Glycine.Ma hope o ka hoʻomaʻemaʻe ʻana, ʻo 97.35% ka maʻemaʻe o ka huahana e ka chromatography wai hana kiʻekiʻe.