ʻO Glycine CAS 56-40-6 (H-Gly-OH) Hōʻike 98.5~101.5% ʻOihana Kiʻekiʻe

USP35-NF30
HOAKAKA
Loaʻa iā Glycine ka NLT 98.5% a me ka NMT 101.5% o ka glycine (C2H5NO2), i helu ʻia ma ke kumu maloʻo.
IKE
A. KA HOPE INFRARED <197M>
HANA
• HANA
Laʻana: 150 mg o Glycine
Palekana: 100 mL o ka ʻakika acetic glacial
Pūnaehana Titrimetric
(E nānā i ka Titrimetry <541>.)
Ke ʻano: Hoʻohālikelike pololei
Titrant: 0.1 N no ka ʻakika chloric VS
ʻIke hope: ʻike
Nānā: E hoʻoheheʻe i ka Laʻana i 100 mL o ka ʻakika acetic glacial, a hoʻohui i 1 kulu o ka TS violet kristal.Titrate me ka Titrant i kahi ʻōmaʻomaʻo hope.Hana i ka hoʻoholo Blank.
E helu i ka pākēneka o ka glycine (C2H5NO2) i ka laʻana i lawe ʻia:
Ka hopena = {[(VS − VB) × N × F]/W} × 100
VS = Ka nui o ka titrant i hoʻopau ʻia e ka Laʻana (mL)
VB = Ka nui o ka pahu i hoʻopau ʻia e ka Blank (mL)
N = ka maʻamau maoli o ka Titrant (mEq/mL)
F = helu kaulike, 75.07 mg/mEq
W = Ke kaumaha laʻana (mg)
Nā mea e ʻae ai: 98.5% -101.5% ma ke kumu maloʻo
NA mea haumia
• KOE MA KA HANA <281>: NMT 0.1%
• CHLORIDE A ME SULFATE, Klorida <221>
Haʻina maʻamau: 0.10 mL o 0.020 N waikona hydrochloric
Laʻana: 1 g o Glycine
Nā pae ʻae ʻae: NMT 0.007%
• CHLORIDE ME SULFATE, Sulfate <221>
Haʻina maʻamau: 0.20 mL o 0.020 N sulfuric acid
Laʻana: 3 g o Glycine
Nā pae ʻae ʻae: NMT 0.0065%
• NA METAL KAUHA, Keʻano I <231>: NMT 20 ppm
• NA METAL KAUHA, Keʻano I <231>: NMT 20 ppm
Laʻana hoʻonā: 100 mg/mL o Glycine
Nānā: E hoʻolapalapa i 10 mL o ka hopena laʻana no 1 min, a waiho ʻia no 2 mau hola.
Paʻa e ʻae ai: ʻIke ʻia ka haʻina e like me ka maopopo a me ke kelepona e like me 10 mL o ka hopena like ʻole i hoʻolapalapa ʻia.
NA HOAO KUAI
• NALO MA KA HOOMALOO <731>: E hoomaloo i kekahi laana ma 105° no 2 h: lilo ia i NMT 0.2% o kona kaumaha.
NA KOI HOU
• PACKAGING a me ka mālama ʻana: mālama i loko o nā pahu paʻa maikaʻi.
• USP REFERENCE STANDARD <11>
USP Glycine RS

Kepani Pharmacopoeia JP17
ʻO Glycine, ke maloʻo, loaʻa ʻaʻole emi ma lalo o 98.5% o Glycine(C2H5NO2).
Hōʻike ʻia ʻo Glycine e like me ke keʻokeʻo, ka pauka orcrystalline kristal.He ʻono kona.Hiki ke hoʻoheheʻe ʻia i ka wai a me ka waikawa formic, a hiki ʻole ke hoʻoheheʻe ʻia i ka ethanol (95).Hōʻike ia i ka polymorphism kristal.
Identification E hoʻoholo i ka spectrum absorption infrared o Glycine, i hoʻomaloʻo mua ʻia, e like me ka kuhikuhi ʻana ma ke ʻano pāpaʻi pāpaʻabromide ma lalo o Infrared Spectrophotometry <2.25>, a e hoʻohālikelike i ka spectrum me ka Reference Spec-trum: hōʻike nā kikoʻī ʻelua i ka ikaika o ka absorption ma nā helu nalu like.Inā ʻike ʻia kekahi ʻokoʻa ma waena o ka spectra, e hoʻoheheʻe i ka Glycine i ka wai, e hoʻoheheʻe i ka wai a maloʻo, a hana hou i ka hoʻāʻo me ke koena.
pH <2.54> E hoʻoheheʻe i 1.0 g Glycine i 20 mL o ka wai: ʻo ka pH o ka hopena ma waena o 5.6 a me 6.6.
Maemae
(1) Ka malamalama a me ka waihoʻoluʻu o ka hoʻonā-Dissolve 1.0 gof Glycine i loko o 10 mL o ka wai: ʻaʻole maopopo ka hopena a ʻaʻole kala.
(2) Chloride <1.03>-E hana i ka ho'āʻo me 0.5 g o Glycine.E hoʻomākaukau i ka hoʻonā mana me 0.30 mL o 0.01mol/L hydrochloric acid VS (ʻaʻole ʻoi aku ma mua o 0.021%)
(3) Sulfate <1.14>-E hana i ka ho'āʻo me 0.6 g o Glycine.E hoʻomākaukau i ka hoʻonā mana me 0.35 mL o 0.005mol/L sulfuric acid VS (ʻaʻole ʻoi aku ma mua o 0.028z).
(4) Ammonium <1.02>-E hana i ka ho'āʻo me ka 0.25 g o Glycine.E hoʻomākaukau i ka hoʻonā hoʻomalu me 5.0 mL o ka ʻĀpana Ammonium maʻamau (ʻaʻole ʻoi aku ma mua o 0.02%).
(5) Nā Metala Kaumaha <1.07>-E hoʻomau me 1.0 g o Glycine e like me ke ʻano 1, a hana i ka hoʻāʻo.E hoʻomākaukau i ka hoʻonā hoʻomalu me 2.0 mL o ka ʻOi (ʻaʻole ʻoi aku ma mua o 20 ppm).
(6) Arsenic <1.11>-E hoʻomākaukau i ka hoʻonā hoʻāʻo me 1.0 gof Glycine e like me ke ʻano 1, a hana i ka hoʻāʻo (ʻaʻole ʻoi aku ma mua o 2 ppm).
(7) Nā mea e pili ana-E hoʻoheheʻe i ka 0.10 g o Glycine i 25mL o ka wai a hoʻohana i kēia hāʻina e like me ka hāpana hāpana.Pipet 1 mL o ka hāpana hāpana, hoʻohui i ka wai e hana pono i 50 mL.Pipet 5 mL o kēia hāpana, hoʻohui i ka wai e hana pololei i 20 mL, a hoʻohana i kēia hoʻonā e like me ka hopena maʻamau.E kau i 5mL o kēlā me kēia hāpana hāpana a me ka hoʻonā maʻamau ma luna o ka pā silica gel forthin-layer chromatography.E hoʻomohala i ka pā me ka hui ʻana o 1-butanol, ka wai a me ka acetic acid (100) (3:1:1) i kahi mamao o 10 cm, a hoʻomaloʻo i ka pā ma 80 ℃ no 30 mau minuke.E pāpaʻi like i ka hopena o ka ninhydrin i loko o ka acetone (1 i ka 50), a me ka wela ma 80 ℃ no 5 mau minuke: ʻaʻole ʻoi aku ka ikaika o nā kiko ma waho o ka wahi nui o ka hāpana hāpana ma mua o ka wahi mai ka hopena maʻamau.
ʻAʻole ʻoi aku ma mua o 0.30% (1 g, 105℃, 3 hola).
ʻAʻole i ʻoi aku ma mua o 0.10% (1g).
Hoʻohāpai pololei ma kahi o 80 mg o Glycine, maloʻo mua, hoʻoheheʻe ʻia i loko o 3 mL o ka waika formic, hoʻohui i 50 mL o ka ʻakika acetic (100), a titrate <2.50> me 0.1 mol/L perchloric acid VS (potentiometric titration).E hana i kahi hoʻoholo ʻokoʻa, a hana i nā hoʻoponopono e pono ai.
ʻO kēlā me kēia mL o 0.1 mol/L perchloric acid VS＝7.507 mg o C2H5NO2
ʻO nā pahu a me ka mālama ʻana i nā ipu-nā ipu pani maikaʻi.