H-Glu(OEt)-OEt·HCl CAS 1118-89-4 L-Glutamic Acid Diethyl Ester Hydrochloride Purity >99.0% (HPLC) Hale Hana

I. Nā kaʻina hana nānā
1. Lae hehee
Hoʻohana ʻia ka mea hoʻoheheʻe kikohoʻe
KaʻAnuʻu 2: Maʻiʻo
2.1 Reagents
KaʻAnuʻu 1: Formic acid
2. Glacial acetic acid
3. ʻO ka waiʻona perchloric maʻamau titration solution (C=0.1000mol/l)
4. ʻO ka wai hōʻailona Violet Crystal: 5g / l.
2.2 Nā mea hana a me nā mea hana
1. Beaker 150ml
2. Microburet: 10ml
2. 3 Kaʻina Hanana
2. 3. Ua kaupaona pololei au ma kahi o 300mg o ka hāpana i hoʻomaloʻo mua ʻia ma 105 ℃ no 3h, e hoʻokomo i loko o ka Betela 150ml, e hoʻohui i 3ml formic acid, e hoʻohui i 50ml o ka wai acetic acetic, e hoʻohui i 2 kulu o ka solution test violet crystal ( TS-74), a e titrate me ka 0.1mol/L perchloric acid a hiki i ka helu hope ʻōmaʻomaʻo a i ʻole a nalo loa ka uliuli.
2. 3. 2 Ke ano helu
Ua like X me C (V1-V2) manawa 0.2042 / M manawa 100
ʻO C ka neʻe ʻana o ka waika perchloric titration solution maʻamau, i hōʻike ʻia ma nā mole no ka lita (mol/l).
ʻO V1 ka leo pau o ka waika perchloric maʻamau titration concentrated solution ma ka laʻana titration, i hōʻike ʻia ma ml (ml).
ʻAi ʻia ka nui o ka waika perchloric titration solution maʻamau ma V2 blank test, i hōʻike ʻia ma ml (ml)
0.2042 a me 1.00ml perchloric acid standard titrated concentrated liquid phase ke hōʻike ʻia ka maikaʻi o kēia huahana ma G
M ʻO ka maikaʻi o ka hāpana, i hōʻike ʻia ma ka gram (g).
2. 4 Hooponopono
Inā ʻoi aku ka ʻokoʻa o ka wela o ka lumi ma waena o ka hopena hoʻokolohua titration a me ka calibration perchloric acid standard solution ma mua o 10 ℃, pono e hoʻoponopono hou ʻia;A i ʻole, e hoʻoponopono ʻia ka ʻike C1 o ka waikona perchloric maʻamau e like me ka hoʻāʻo (2).
C2= C1/1+0.0011 (t1-t2)
ʻO 1+0.0011 ka helu hoʻonui o ka waikawa acetic glacial
ʻO ka ʻāpana o ka wela lumi no ka calibration T2 o ka waikawa perchloric ʻo Celsius (° C).
ʻO Celsius (° C) ka ʻāpana o ka wela lumi kahi i hoʻopaʻa ʻia ai kahi hāpana.
C1 t2 ℃ perchloric acid ka hoʻonā kūlana maʻamau, ma nā mole no ka lita (MOL/L)
3. Maloo a kaumaha ole
3.1 Mea Hana:
ʻO ka umu hoʻomaloʻo Thermostatic, 1/10,000 koena.
3.2 Kaʻina hana:
E lawe i 1-2 grams o nā laʻana (pololei i ka 0.5mg) a hoʻokomo iā lākou i loko o kahi ʻōmole paona mau i hoʻomaloʻo ʻia a kaupaona ʻia.Loaʻa ka mānoanoa like ʻole o nā laʻana ma lalo o ka ʻōmole kaupaona.No nā laʻana, e hoʻomaloʻo ʻia lākou i loko o kahi umu thermoelectric (105±5 ℃) i ke kaumaha mau.
3.3 Heluhelu
ʻO X ka m me ka m1 puunaue me ka m me ka m2 manawa 100
Kahi: X- maʻiʻo mākū %
m1 -- Ke kaumaha o ka hāpana maloʻo me ka ʻōmole ana g
m2 - E ana i ka nui o ka omole g
m- Ke kaumaha o ka hāpana g
3.4 Manao
1. Manaʻo: 1. Pono e hana ʻia nā laʻana like no ka maloʻo ʻana a me ke kaumaha ʻole.ʻElua mau hopena i hoʻokahi laʻana, a ʻo ka hapa ma waena o nā hopena ʻelua ʻaʻole ʻoi aku ma mua o 0.5%.
2. Pono e akahele ke kaupaona, pololei a hiki i 0.0001.
4 i ka hoohuli ana
4.1 Nā Kumu
ʻO ke kukui polarized plane e hele ana i loko o ka wai a i ʻole ka hopena o kahi pūhui i loaʻa i kekahi hana optical (ʻo ia hoʻi, kahi mea optically active) hiki ke hoʻololi i ka ʻōuli ʻana, e hoʻohuli i ka mokulele o ke kukui polarized i ka hema a i ʻole.ʻO ka helu o nā degere o ka hoʻololi ʻana i kapa ʻia ʻo optical rotation.Ma kahi mahana (i hōʻike pinepine ʻia e t, hiki ke 20 ℃ a i ʻole 25 ℃) kekahi mau nalu o ka mālamalama (ke kukui sodium melemele me D, ka lōʻihi hawewe λ 589.3nm) kukui polarized ma o kēlā me kēia milliliter i loaʻa i ka 1g optical activity material, ka mānoanoa o 1dm. (ʻo ia hoʻi, 10cm) hoʻonā i ka wā i kapa ʻia ai ka ʻōwili ʻana i ka huli ʻana o ka optical kikoʻī.
4.2 Koi
Ua hoʻohana ʻia ka laina D o ka sodium spectrum (589. 3 nm) e ana i ka lōʻihi o ka paipu o 1dm a ʻo ka mahana he 20 ℃.
4.3 Mea Hana
4.3.1 Kikohoʻe autotropy (pololei ±0.001°)
4.3.2 Kaulike uila (pololei 0.0001g).
4.4 Hana Hana
E lawe i 3g o kēia huahana, pololei i ka 0.0001g, e hoʻokomo i loko o ka ʻōmole volumetric 100ml, e hoʻohui i ka ethanol e hoʻoheheʻe a hoʻoheheʻe ʻia i ka unahi, e lulu maikaʻi.Holoi i ka paipu ana me ka li'ili'i o ka wai no nā manawa he nui, a laila, ho'okomo mālie i ka wai i loko o ka paipu ana ('a'ole e ho'oulu 'ia), e kau i ka paipu ana i loko o ka gyroscope no ka 'ike 'ana, a heluhelu i ka heluhelu 'ana i ka huli 'ana. 3 manawa like.
4.5 Heluhelu
ʻO ka hoʻololi kikoʻī o L-glutamate Diethyl carbonate i ka sodium spectrum D-line ma 20 ℃ [Alpha] D20 i helu ʻia penei:
[ɑ] D20 = alpha/m
Kahi: α -- helu awelika o nā heluhelu hoʻololi cubic;
m -- maikaʻi hāpana.
4.6 ʻOkoʻa ʻae ʻia: ʻAʻole ʻoi aku ka ʻokoʻa loa o ka hāpana like ma mua o 0.02% ke ana ʻelua.
4.7 Hoʻomalu
4.7.1 Pono e hoʻohana ʻia ka mea hoʻoheheʻe e like me ka calibration blank ma mua o kēlā me kēia ana: Ma hope o ke ana ʻana, pono e hana hou ʻia ka calibration no ka hoʻoholo ʻana inā hoʻololi ke kiko ʻole i ke ana ʻana.Inā loaʻa kekahi mau loli, pono e hoʻoholo hou i ka huli ʻana o ka hāpana.
4.7.2 Pono e wehewehe ʻia ka hāpana ma hope o ka hoʻoheheʻe ʻana a me ka dilution;a i ʻole, pono e kānana ʻia.
KaʻAnuʻu 5 Nānā
He keʻokeʻo ka pauka microcrystalline keʻokeʻo ka huahana