L-4-Nitrophenylalanine Methyl Ester HCl CAS 17193-40-7 H-Phe(4-NO2)-OMe·HCl Assay >98.5% (HPLC) Factory

Ke Kaʻina Hana o ka L-4-Nitrophenylalanine Methyl Ester HCl (H-Phe(4-NO2)-OMe·HCl) (CAS: 17193-40-7)
Wehewehe: E kau ma kahi o 2 g o ka laʻana i loko o kahi ipu petri-dish maʻemaʻe a maloʻo a nānā i ke ʻano o ka laʻana.Hōʻike: E hōʻike i ka nānā
Identificaiton: E hoʻohālikelike i ka manawa paʻa o ka piko nui ma ka chromatogram o ka hoʻomākaukau hoʻāʻo me ka manawa paʻa o ka piko nui i ka chromatogram o ka praparation kūpono ʻōnaehana e like me ka loaʻa ʻana o ka maʻemaʻe chromatographic e HPLC.
Pohō ma ka hoʻomaloʻo ʻana: E hoʻomaloʻo i ka ʻōmole kaupaona pāpaʻu i pani ʻia i ke aniani ma 105±2 ℃ no 30 mau minuke.E ʻae i ka maʻalili a hiki i ka lumi wela i loko o kahi desciccator.E kaupaona pono i ka omole kaumaha me ka pahu a e kakau i ke kaumaha (W1).E hoʻoili ma kahi o 1 a 2g ka nui o ka mea hoʻāʻo i kaupaona pololei ʻia i loko o ka ʻōmole kaupaona.Me ka lulu mālie a me ka ʻaoʻao ʻaoʻao, e puʻunaue i ke ʻano hoʻāʻo e like me ka hiki i ka hohonu o 5 mm paha.E kaupaona i ka omole kaumaha i hookomoia a e kakau i ke kaumaha (W2).E kau i ka ʻōmole i hoʻouka ʻia i loko o ka umu maloʻo, e wehe i ka pani a waiho pū i loko o ke keʻena.E hoʻomaloʻo i ka hāpana hoʻāʻo ma 105±2 ℃ no 3 mau hola.I ka wehe ʻana i ke keʻena, e pani koke i ka ʻōmole, a e ʻae iā ia e hele i kahi mahana i loko o kahi desiccator.E kaupaona pono i ka omole kaupaona me ka pani a ho'i hou i ke kaumaha (W3).E helu i ka pakeneka poho ma ka maloʻo ʻana o ka hāpana ma o ka hoʻohana ʻana i ke ʻano:
Helu helu ma ka maloo (% w/w)= (W2-W3) ÷ (W2-W1) x 100
Nā ʻike Enantiomer na HPLC:
Kemika & Reagents:
n-Hexane AR māka
Papa Methanol HPLC
Isopropyl Alcohol HPLC māka
Papa Diethylamine AR
Nā Kūlana: L-4-Nitrophenylalanine Methyl Ester HCl standard;L-4-Nitrophenylalanine Methyl Ester HCl enantiomer maʻamau
Māhele Mobile: E hoʻomākaukau i kahi hui like o n-Hexane, Isopropyl Alcohol, Methanol a me Diethylamine (65:20:15:0.1 v/v/v/v)
Hoʻomākaukau Paʻa: E hoʻololi i ka 2.5ml o ka Methanol i loko o kahi pahu volumetric 10-ml, e hoʻoheheʻe i ka leo me ka pae kelepona a hui pū.
Hoʻomākaukau kūpono ʻōnaehana:
E kaupaona pololei a hoʻololi i ka 5 mg o kēlā me kēia L-4-Nitrophenylalanine Methyl Ester HCl maʻamau a me L-4-Nitrophenylalanine Methyl Ester HCl enantiomer maʻamau i loko o ka pahu volumetric 10-ml, e hoʻoheheʻe i loko o 2.5ml o ka methanol, e hoʻoheheʻe i ka leo me ka mobile. pae a hui.
Hoʻomākaukau hoʻāʻo:
E kaupaona pololei a e hoʻololi i ka 5 mg o ka laʻana i loko o kahi pahu volumetric 10-mol, e hoʻoheheʻe ʻia ma kahi o 2.5ml o ka methanol, e hoʻoheheʻe ʻia i ka leo me ka pae kelepona a hui pū.
Nā ʻāpana mea kani:
Mea hana: HPLC i lako me ka injector, Pump, UV detector and Recorder
Kona: ChiralPak-IC (4.6mm x 250mm) 5 μ a i ʻole like
Kahe kahe: 1.0 ml / minuke
Loʻihi nalu: 260nm
Ka nui o ka hoʻokomo ʻana: 10 μL
Ke ana wela o ke kolamu: 25℃
Ka manawa holo: 20 minuke
Kaʻina hana
Hoʻokomo i nā hāʻina e like me ka mea i ʻōlelo ʻia ma ke kaʻina aʻe.E hoʻopaʻa i ka chromatogram a hōʻoia i nā pae hoʻohālike:
ʻO ka wehewehe ʻana o nā Injections Ka helu o nā Injections Criteria
Hoʻomākaukau Pākaʻa 1 ʻAʻole pono ke nānā ʻia kahi kiʻekiʻe hoʻopilikia i ka manawa paʻa o L-4-Nitrophenylalanine Methyl Ester HCl a i ʻole enantiomer.
ʻO ka hoʻomākaukau kūpono ʻana o ka ʻōnaehana 1 ʻAʻole i emi iho ka hopena ma waena o L-4-Nitrophenylalanine Methyl Ester HCl a me ka piko enantiomer ma lalo o 2.0
Hoʻomākaukau hoʻāʻo 1 E hoʻohana i ka chromatogram hoʻāʻo e hoʻoholo i ka ʻike enantiomer i loko o ka hāpana.
Nā manawa paʻa pili:
L-4-Nitrophenylalanine Methyl Ester HCl 1.0
Enantiomer Ma kahi o 1.11
ʻO ka manawa paʻa o L-4-Nitrophenylalanine Methyl Ester HCl ma kahi o 9.8 mau minuke.
Heluhelu: Ma ka chromatogram i loaʻa mai ka hoʻomākaukau hoʻāʻo ʻana, e hoʻowahāwahā i nā piko āpau e manaʻo ʻia ʻo L-4-Nitrophenylalanine Methyl Ester HCl a me Enantiomer.E hōʻike i ka maʻiʻo enantiomer i loko o ka hāpana ma ke ʻano hana maʻamau.
Chromatogrpahic Maʻemaʻe a me nā haumia e HPLC:
Kemika & Reagents:
Diammonium phosphate AR māka
ʻAkika Phosphoric AR māka
Papa Methanol HPLC
Wai Hoomaemae
Nā kūlana
L-4-Nitrophenylalanine Methyl Ester HCl maʻamau
4-Nitro-L-Phenylalanine monohydrate maʻamau
Māhele Mobile-A
E hoʻoheheʻe i 6.6 g o Diammonium phosphate i 1000ml o ka wai maʻemaʻe.Hoʻoponopono i ka pH o kēia hoʻonā i 6.0 me ka waikawa Phosphoric.Kānana a me ka degas.Ma mua o ke ana ʻana i ka pH o ka hopena, e hoʻopololei i ka mika pH me ka solution buffer maʻamau pH 4.01 ME 7.00.
Māhele-B
Papa Methanol HPLC
Hoʻomākaukau Pono Pūnaewele
E kaupaona pololei a e hoʻololi i 25 mg kēlā me kēia L-4-Nitrophenylalanine Methyl Ester HCl maʻamau a me 4-Nitro-L-Phenylalanine monohydrate maʻamau i loko o ka pahu volumetric 50-ml, e hoʻoheheʻe i loko a hoʻoheheʻe i ka leo me ka methanol a hui pū.
Hoʻomākaukau hoʻāʻo:
E kaupaona pololei a e hoʻololi i kahi laʻana 25 mg i loko o kahi ipu volumetric 50-ml, e hoʻoheheʻe i loko a hoʻoheheʻe ʻia me ka methanol a hui pū.
Nā ʻāpana mea kani:
Mea hana: HPLC i lako me ka injector, Pump, UV detector and Recorder
Kona: XTerra RP-18 (4.6mm x 150mm) 5 μ a i ʻole like
Kahe kahe: 1.0 ml / minuke
Loʻihi nalu: 265nm
Ka nui o ka hoʻokomo ʻana: 20 μL
Ke ana wela o ke kolamu: 25℃
Ka manawa holo: 45 minuke
Polokalamu Gradient
Manawa (min) Māhele Paʻa-A % Māhele Paʻa-B %
0.01 90 10
15 60 40
35 40 60
40 35 65
41 90 10
45 90 10
Kaʻina hana:
Hoʻokomo i nā hāʻina e like me ka mea i ʻōlelo ʻia ma ke kaʻina aʻe.E hoʻopaʻa i ka chromatogram a hōʻoia i nā pae hoʻohālike:
Hoʻomākaukau Pākaʻa 1 ʻAʻole pono ke nānā ʻia kahi peak interfering i ka manawa paʻa o L-4-Nitrophenylalanine Methyl Ester HCl.
ʻO ka hoʻomākaukau kūpono ʻana o ka ʻōnaehana 1 ʻAi ʻole ka liʻiliʻi ma lalo o ka 2.0 kumu no ka L-4-Nitrophenylalanine Methyl Ester HCl.
Hoʻomākaukau hoʻāʻo 1 E hoʻohana i ka chromatogram hoʻāʻo e hoʻoholo i ka maʻemaʻe chromatographic a me nā haumia.
Nā manawa paʻa pili:
L-4-Nitrophenylalanine Methyl Ester HCl 1.0 (manawa paʻa ma kahi o 11.4 mau minuke)
4-Nitro-L-Phenylalanine Ma kahi o 0.4
Toluene Ma kahi o 2.3
Heluhelu:
I ka chromatogram i loaʻa mai ka hoʻomākaukau hoʻāʻo ʻana, ma hope o ka hoʻoponopono ʻole ʻana, e hoʻowahāwahā i ka piko ma muli o toluene.E hōʻike i ka maʻemaʻe chromatographic, 4-Nitro-L-Phenylalanine maʻiʻo a me nā haumia a pau o ka hāpana ma ke ʻano hana maʻamau.