L-Cysteine ​​CAS 52-90-4 (H-Cys-OH) Assay 98.5~101.0% (Titration) Hale Hana Mea Ki'eki'e

ʻAʻole i emi iho ka L-Cysteine ​​ma lalo o 98.5% a ʻaʻole i oi aku ma mua o 101.0% o L-Cysteine ​​(C3H7NO2S), i helu ʻia ma ke kumu maloʻo.
Hōʻike ʻia ʻo L-Cysteine ​​​​ma ke ʻano he keʻokeʻo keʻokeʻo a i ʻole ka pauka crystalline keʻokeʻo.He ʻala ʻano a me ka ʻono ʻono.Hiki ke hoʻoheheʻe ʻia i ka wai, a hiki ʻole ke hoʻoheheʻe ʻia i ka ethanol (99.5).Hoʻoheheʻe ia i ka 1 mol/L hydrochloric acid TS.
Identification E hoʻoholo i ke kiko kikoʻī infrared absorption o L-Cysteine ​​e like me ke kuhikuhi ʻana ma ke ʻano pāpaʻi bromide pāpaʻa ma lalo o Infrared Spectrophotometry <2.25>, a hoʻohālikelike i ke kikoʻī me ka Spectrum Reference: hōʻike nā kikoʻī ʻelua i ka ikaika o ka absorption ma nā helu nalu like.
ʻO ka hoʻololi ʻana o ka Optical <2.49> [a]20D:+8.0 ~+10.0° (2 g helu ʻia ma ke kumu maloʻo, 1 mol/L hydrochloric acid TS, 25mL, 100 mm).
pH <2.54> ʻO ka pH o kahi hopena i hoʻomākaukau ʻia ma ka hoʻoheheʻe ʻana i 1.25 g o L-Cysteine ​​​​i loko o 50 mL o ka wai he 4.7 a 5.7.
Maʻemaʻe (1) Maʻemaʻe a me ka waihoʻoluʻu o ka hoʻonā-Dissolve 1.0 g o L-Cysteine ​​​​i loko o 20 mL o ka wai: maopopo ka hopena a me ke kala ʻole.
(2) Chloride <1.03>-Dissolve 0.30 g o L-Cysteine ​​​​i loko o 10mL o ka waikawa nitric diluted (1 i ka 4), hoʻohui i 10mL o hydrogenperoxide (30), wela no 20 mau minuke i loko o kahi ʻauʻau wai paila, maloʻo, a laila hoʻohui. wai e hana i 50 ml.Hana i ka ho'āʻo me ka hoʻohana ʻana i kēia hoʻonā e like me ka hopena hoʻāʻo.E hoʻomākaukau i ka hoʻonā mana me ka 0.35 mL o 0.01 mol/L hydrochloric acid VS (ʻaʻole ʻoi aku ma mua o 0.041%)
(3) Sulfate <1.14>-E hoʻoheheʻe i 0.6 g o L-Cysteine ​​​​i loko o 30 mL o ka wai a me 3 mL o ka waika hydrochloric dilute, a hoʻohui i ka wai e lilo i 50 mL.Hana i ka ho'āʻo me ka hoʻohana ʻana i kēia hoʻonā e like me ka hopena hoʻāʻo.E hoʻomākaukau i ka hopena hoʻomalu penei: I ka 0.35 mL o 0.005 mol/L sulfuric acid VS e hoʻohui i 3 mL o ka waika hydrochloric dilute a me ka wai e lilo i 50 mL.E hoʻomākaukau i ka hopena ho'āʻo a me ka hopena hoʻomalu me 4 mL o ka barium chloride TS, i kēlā me kēia (ʻaʻole iʻoi aku ma mua o 0.028%).
(4) Ammonium <1.02>-E hana i ka ho'āʻo me 0.25 g o L-Cysteine, me ka hoʻohana ʻana i ka distillation ma lalo o ke kaomi hoʻemi.E hoʻomākaukau i ka hoʻonā hoʻomalu me 5.0 mL o ka ʻĀpana Ammonium maʻamau (ʻaʻole ʻoi aku ma mua o 0.02%).
(5) Heavy Metals <1.07>-E hoʻomau me ka 1.0g o L-Cysteine ​​​​e like me keʻano 4, a hana i ka ho'āʻo. E hoʻomākaukau i ka hoʻonā mana me 1.0 mL o ka Hoʻoponopono Lead Maʻamau (ʻaʻole iʻoi aku ma mua o 10 ppm).
(6) Ka hao <1.10>-E hoʻomākaukau i ka hopena ho'āʻo me 1.0 g o L-Cysteine ​​​​e like me keʻano 1, a hana i ka ho'āʻo e like me keʻano A. ).
(7) Nā mea pili-E hoʻoheheʻe i 0.10 g o L-Cysteine ​​​​i loko o ka solution N-ethylmaleimide (1 i ka 50) e hana pono i 10 mL, waiho no 30 mau minuke, a hoʻohana i kēia hopena ma ke ʻano he hāpana hāpana.Pipet 1 mL o ka hāpana hāpana, hoʻohui i ka wai e hana pono ai i 10 mL, pipet 1 mL o kēia hāʻina, hoʻohui i ka wai e hana pono i 50 mL, a hoʻohana i kēia ʻano hoʻonā ma ke ʻano he hopena maʻamau (1).E hoʻokaʻawale, e hoʻoheheʻe i 0.10 g o L-cystine i 0.5mol/L hydrochloric acid TS e hana pono i 20 mL.Pipet 1mL o kēia haʻina, hoʻohui i ka wai e hana i 100 mL, a hoʻohana i kēia haʻina e like me ka hopena maʻamau (2).E hana i ka ho'āʻo me kēia mau haʻina e like me ka kuhikuhi ʻana ma lalo o Thin-layer Chromatography<2.03>.E kau i 10mL i kēlā me kēia o ka hāpana hāpana a me nā haʻina maʻamau (1) a me (2) ma luna o kahi pā silica gel no ka chromatography ʻāpana lahilahi.E hoʻomohala i ka pā me ka hui ʻana o 1-butanol, ka wai, a me ka acetic acid (100) (3:1:1) i kahi mamao o 10 knm, a hoʻomaloʻo i ka pā no 30 mau minuke ma 809C. E pīpī i ka pā me ka hopena. o ninhydrin i loko o ka hui ʻana o ka methanol a me ka ʻakika acetic (100) (97:3) (1 i ka 100), a laila wela ma 80 ℃ no 10 mau minuke: kahi i loaʻa mai ka hāpana hāpana e pili ana i kahi i loaʻa mai ka hopena maʻamau. ʻAʻole ʻoi aku ka ikaika o (2) ma mua o ka wahi mai ka hopena maʻamau (2).Eia kekahi, ʻaʻole ʻoi aku ka ikaika o nā kiko ʻē aʻe ma waho o ke kiko nui a me nā kiko i ʻōlelo ʻia ma luna o ka hāpana hāpana ma mua o ke kiko mai ka solution maʻamau (1).
ʻAʻole i ʻoi aku ma mua o 0.50% (1 g, i loko o ka vacuum, phosphorus (V) oxide, 3 mau hola).
ʻAʻole i ʻoi aku ma mua o 0.1%(1 g).
E kaupaona pololei ma kahi o 0.2 g o L-Cysteine, e hoʻokomo i loko o ka ipu i pani ʻia, a e hoʻoheheʻe ʻia i loko o 20 mL o ka wai.E hoʻoheheʻe i 4 g o ka potassium iodide i loko o kēia hopena, e waiho koke i loko o ka wai anuanu, e hoʻohui i 5 mL o ka waika hydrochloric dilute a me 25 mL pono o 0.05 mol/L iodine VS, waiho i kahi pouli no 20 mau minuke, a laila titrate <2.50 > ka nui o ka iodine me 0.1 mol/L sodium thiosulfate VS (hōʻailona: starch TS).Hana i kahi hoʻoholo hakahaka me ke ʻano like.
ʻO kēlā me kēia mL o 0.05 mol/L iodine VS＝12.12 mg o C3H7NO2S
ʻO nā pahu a me nā pahu waihona-nā pahu paʻa.