Levodopa (L-DOPA) CAS 59-92-7 99.0~100.5% USP BP EP Ka ma'i Anati-Parkinson ma'amau.

ʻO ka hoʻololi ʻana [EP]
E hoʻoheheʻe i ka nui like me 0.200g o ka mea maloʻo a me 5g o hexamethylenetetramine R i loko o 10ml o 1mol/L hydrochloric acid a hoʻoheheʻe i 25.oml me ka waikawa like.E ʻae i ka hoʻonā e kū i pale ʻia mai ka mālamalama no 3h.ʻO ke kihi o ka huli ʻana o ka optical he -1.27° a i -1.34°
ʻIke ʻia o ka Haʻina
Disslove 1.0g i ka 1mol/L hydrochloric acid a dilute i 25ml me ka solvnet like.ʻAʻole ʻoi aku ka ikaika o ka waihoʻoluʻu ma mua o ka hoʻonā kuhikuhi BY6.
Nā mea pili [EP]
E noʻonoʻo me ka chromatography ʻāpana lahilahi, me ka hoʻohana ʻana i ka cellulose no ka chromatography R ma ke ʻano he mea uhi.
ʻO ka hopena hoʻāʻo-E hoʻoheheʻe i 0.1g o ka mea e nānā ʻia i loko o 5ml o ka anhydrous formic acid R a hoʻoheheʻe i 10ml me ka methanol R. E hoʻomākaukau koke ma mua o ka hoʻohana ʻana.
Haʻina kuhikuhi (a)-E hoʻoheheʻe i 0.5ml o ka hopena hoʻāʻo i 100ml me ka methanol R.
Pane kuhikuhi (b)-E hoʻoheheʻe i 30mg o tyrosine R i 1ml o ka waika formic anhydrous R a hoʻoheheʻe ʻia i 100ml me ka methanol R. E hoʻohui i 1ml o kēia wai me 1ml o ka hopena hoʻāʻo.
E hoʻopili paʻa i ka pā e like me nā kaula 20mm ka lōʻihi, 1oμl o ka hoʻonā hoʻāʻo, 10μl o ka hopena kuhikuhi (a) a me 20μl o ka hopena kuhikuhi (b).Maloo i ke au o ka ea.E hoʻomohala ma luna o kahi ala o 15cm me ka hoʻohana ʻana i ka hui ʻana o 50 mau puke o ka butanol R, 25 mau puke o ka ʻakika acetic glacial R a me 25 mau puke wai.E hoʻomaloʻo i ka pā i loko o ke ea mehana a kāpīpī me kahi hui hou i hoʻomākaukau ʻia o ka nui like o ka 10% m/v solution of ferric chloride R a me 5 percent m/v solution of postassium ferricyanide R. E nānā koke i nā chromatoframs.Kekahi wahi o ka chromatogram i loaʻa me ka hoʻonā hoʻāʻo, ʻokoʻa ka wahi nui, ʻaʻole i ʻoi aku ka ikaika ma mua o ka wahi o ka chromatogram i loaʻa me ka hoʻonā kuhikuhi (a) hōʻike.ʻAʻole kūpono ka hoʻāʻo inā ʻaʻole hōʻike ka chromatogram i loaʻa me ka solution reference (b), ma luna o ke kiko nui, kahi wahi ʻokoʻa i ʻoi aku ka ikaika ma mua o ke kiko o ka chromatogram i loaʻa me ka solution reference (a).
ʻAno UV [EP]
Disslove 30.0mg i ka 0.1mol/L hydrochloric acid a dilute i 100.oml me ka waika like.E hoʻoheheʻe i 10.0ml o kēia hoʻonā i 100.0ml me 0.1mol/L hydrochloric acid.Nānā ʻia ma waena o 230nm a me 350nm, hōʻike ka hopena i hoʻokahi absorption maximum ma 280nm.ʻO ka absorbance kikoʻī ma kēia kiʻekiʻe he 137 a 147, i helu ʻia me ka kuhikuhi ʻana i ka mea maloʻo.
Nalo ma ka maloo
E lawe i kēia huahana, maloʻo a hiki i ke kaumaha mau ma 105°C, ʻaʻole e ʻoi aku ke kaumaha o ke kaumaha ma mua o 1.0% (General rule 0831).
ʻO ke koena ma ka ʻāʻī (Sulfated)
E lawe i ka l.0g o Levodopa a nānā e like me ke kānāwai (General rule 0841).ʻAʻole ʻoi aku ke koena o ke koena ma mua o 0.1%.
Nā Metala Kaumaha
ʻO ke koena i waiho ʻia ma lalo o ke ʻano o ka lawe ʻana i ke koena hoʻā ahi ʻaʻole e loaʻa ma mua o 10 mau ʻāpana o ka miliona o ka metala kaumaha ke nānā ʻia e ke kānāwai (General Principles 0821, Law II).
Hōʻike pH
0.10g i 10ml o H2O lulu no 15 minuke.
Hoʻoholo maʻiʻo
E lawe i kēia huahana e pili ana i 0.lg, pololei ke kaupaona ʻana, e hoʻohui i ka anhydrous formic acid 2ml e hoʻoheheʻe, e hoʻohui i ka glacial acetic acid 20ml, lulu, hoʻohui i ka aniani violet hōʻailona 2 kulu, me ka perchloric acid titration solution (0.1 mol/L) titration i ka hopena ʻōmaʻomaʻo, a hoʻoponopono ʻia ka hopena o ka titration me kahi hoʻāʻo blank.ʻO kēlā me kēia 1 ml o ka waika perchloric titration solution (0.1 mol/L) pili i 19.72mg o C9H11N04.

JP17 Hana Hana

ʻO Levodopa, ke maloʻo, ʻaʻole i emi iho ma lalo o 98.5% o Levodopa (C9H11NO4).
Hōʻike ʻia ʻo Levodopa ma ke ʻano keʻokeʻo a i ʻole keʻokeʻo hina hina, nā kristal a i ʻole ka pauka crystalline.He ala ʻole.Hiki ke hoʻoheheʻe ʻia i ka waika formic, hiki ke hoʻoheheʻe ʻia i ka wai, a hiki ʻole ke hoʻoheheʻe ʻia i ka ethanol (95).Hoʻoheheʻe ia i ka waika hydrochloric dilute.Aia ma waena o 5.0 a me 6.5 ka pH o ka hopena saturated o Levodopa.Ka helu hehee: ma kahi o 275 ℃ (me ka decomposition).
ʻIkepili
(1) I ka 5 mL o ka hopena o Levodopa (1 i ka 1000) hoʻohui i 1mL o ninhydrin TS, a wela no 3 mau minuke i loko o ka ʻauʻau wai: ulu ka waihoʻoluʻu poni.
(2) I ka 2 mL o ka hopena o Levodopa (1 i loko o 5000) hoʻohui i 10 mL o 4-aminoantipyrine TS, a lulu: ulu ka ʻulaʻula.
(3) E hoʻoheheʻe i 3 mg o Levodopa i 0.001 mol/L hydrochloric acid TS e hana i 100 mL.E hoʻoholo i ke kiko kikoʻī o ka haʻina e like me ke kuhikuhi ʻana ma lalo o Ultravioletvisible Spectrophotometry <2.24>, a e hoʻohālikelike i ke kikoo me ka Spectrum Reference: hōʻike nā kikoʻī ʻelua i ka ikaika o ka absorption ma nā lōʻihi nalu like.
Absorbance <2.24> E 1%1cm (280 nm): 136-146 (ma hope o ka maloʻo, 30 mg, 0.001 mol / L hydrochloric acid TS, 1000 mL).
Hoʻololi Optical <2.49> [a]20D:-11.5° ~-13.0°(ma hope o ka maloʻo ʻana, 2.5 g, 1 mol/L hydrochloric acid TS, 50 mL, 100 mm).
Maemae
(1) Ka malamalama a me ka waihoʻoluʻu o ka hoʻonā-Dissolve 1.0 g o Levodopa i 20 mL o 1 mol/L hydrochloric acid TS: he akaka a kala ʻole ka hopena.
(2) Chloride <1.03>-E hoʻoheheʻe i 0.5 g o Levodopa i 6 mLof dilute nitric acid, a hoʻohui i ka wai e lilo i 50 mL.Hana i ka ho'āʻo me ka hoʻohana ʻana i kēia hoʻonā e like me ka hopena hoʻāʻo.E hoʻomākaukau i ka hopena hoʻomalu me 0.30 mL o 0.01 mol/L hydrochloricacid VS (ʻaʻole iʻoi aku ma mua o 0.021%).
(3) Sulfate <1.14>-E hoʻoheheʻe i 0.40 g Levodopa i 1 mL o ka waika hydrochloric dilute a me 30 mL o ka wai, a hoʻohui i ka wai e lilo i 50 mL.Hana i ka ho'āʻo me ka hoʻohana ʻana i kēia hoʻonā e like me ka hopena hoʻāʻo.E hoʻomākaukau i ka hoʻonā mana me 0.25 mL o 0.005 mol/L sulfuric acid VS (ʻaʻole ʻoi aku ma mua o 0.030%).
(4) Nā Metala Kaumaha <1.07>-E hoʻomau me 1.0 g o Levodopa e like me ke ʻano 2, a hana i ka hoʻāʻo.E hoʻomākaukau i ka hoʻonā hoʻomalu me 2.0 mL o ka ʻOi (ʻaʻole ʻoi aku ma mua o 20 ppm).
(5) Arsenic <1.11>-Dissolve 1.0 g o Levodopa i loko o 5 mLof dilute hydrochloric waika, a hana i ka ho'āʻo me kēia hāʻina e like me ka ho'āʻo hoʻonā (ʻaʻole i oi ma mua o 2 ppm).
(6) Nā mea pili-E hoʻoheheʻe i 0.10 g o Levodopa i 10 mL o ka sodium disulfite TS, a hoʻohana i kēia hoʻonā e like me ka hāpana hāpana.Pipet 1 mL o ka hāpana hāpana, hoʻohui i ka sodium disulfite TS e hana pono i 25 mL.Pipet 1 mL o kēia hoʻonā, e hoʻohui i ka sodium disulfite TS e hana pono i 20 mL, a e hoʻohana i kēia hopena ma ke ʻano he hopena maʻamau.Hana i ka ho'āʻo me kēia mau hoʻonā e like me ke kuhikuhi ʻana ma lalo o Thin-layer Chro-matography<2.03>.E kau i ka 5mL o kēlā me kēia hāpana hāpana a me ka hopena maʻamau ma luna o kahi pā cellulose no ka chromatography lahilahi.E hoʻomohala i ka pā me ka hui ʻana o 1-butanol, wai, acetic acid (100) a me methanol (10:5:5:1) a hiki i kahi mamao o 10 cm, a maloʻo i ka pā.E kāpīpī i ka hopena o ka ninhydrin i loko o ka acetone (1 i ka 50) ma ka pā a me ka wela ma 90 ℃ no 10 mau minuke: ʻaʻole i ʻoi aku ka ikaika o nā kiko ma mua o ka wahi nui o ka hāpana hāpana.
ʻAʻole ʻoi aku ma mua o 0.30%(1 g, 105℃,3 hola).
ʻAʻole i ʻoi aku ma mua o 0.1% (1 g).
E kaupaona pololei ma kahi o 0.3 g o Levodopa, maloʻo mua, e hoʻoheheʻe i 3 mL o ka waika formic, e hoʻohui i 80 mL o ka ʻakika acetic (100), a e hoʻokaʻawale i <2.50> me 0.1 mol/L perchloric acid VS a hiki i ka hoʻololi ʻana o ke kala o ka hopena. mai ka poni a hiki i ka uliuli-'ōmaʻomaʻo a i ka ʻōmaʻomaʻo (hōʻailona: 3 kulu o crystalviolet TS).Hana i kahi hoʻoholo hakahaka, a hana i nā hoʻoponopono e pono ai.
ʻO kēlā me kēia mL o 0.1 mol/L perchloric acid VS＝19.72 mg o C9H11NO4
ʻO nā pahu a me ka mālama ʻana i nā pahu pahu-paʻa paʻa.
Waihona-kū-māmā.