L-Glutamine CAS 56-85-9 (H-Gln-OH) Ho'āʻo 99.0~101.0% Hale Hana Kiʻekiʻe

Pharmacopoeia KepaniNā Kūlana Kūlana
L-Glutamine [56-85-9]
ʻO L-Glutamine, ke maloʻo, ʻaʻole i emi iho ma lalo o 99.0% a ʻaʻole ʻoi aku ma mua o 101.0% o L-Glutamine (C5H10N2O3).
Hōʻike ʻia ʻo L-Glutamine ma ke ʻano he keʻokeʻo, nā kristal a i ʻole ka pauka crystalline.He ʻono ʻano liʻiliʻi kona
Hiki ke hoʻoheheʻe ʻia i ka waika formic, hiki ke hoʻoheheʻe ʻia i ka wai, a hiki ʻole ke hoʻoheheʻe ʻia i ka ethanol (99.5)
Identification E hoʻoholo i ke kikoʻī o ka infrared absorption o L-Glutamine e like me ke kuhikuhi ʻana ma ke ʻano pāpaʻi bromide pāpaʻa ma lalo o Infrared Spectrophotometry <2.25>, a hoʻohālikelike i ke kikoʻī me ka Spectrum Reference: hōʻike nā kikoʻī ʻelua i ka ikaika o ka absorption ma nā helu nalu like.
ʻO ka Rotation Optical <2.49> [a]20D:+6.3° a i +7.3° E kaupaona pololei ma kahi o 2 g o L-Glutamine, i maloʻo mua, e hoʻohui i 45mL o ka wai, mahana a 40 ℃ e hoʻoheheʻe, a ma hope o ka hoʻoluʻu ʻana, hoʻohui i ka wai e hana ai. pololei 50 mL.E hoʻoholo i ka hoʻololi ʻana o kēia hoʻonā i loko o ke kelepona 100-mm, i loko o 60 mau minuke.
pH <2.54> ʻO ka pH o kahi hopena i hoʻomākaukau ʻia ma ka hoʻoheheʻe ʻana i 1.0 g o L-Glutamine i 50 mL o ka wai ma waena o 4.5 a me 6.0.
Maemae
(1) Malamalama a me ka waihoʻoluʻu o ka hoʻonā—ʻO kahi hopena i loaʻa ma ka hoʻoheheʻe ʻana i ka 0.5 g o L-Glutamine i loko o 20 mL o ka wai maʻemaʻe a kala ʻole.
(2) Chloride <1.03> —E hana i ka ho'āʻo me 0.5 g o L-Glutamine.E hoʻomākaukau i ka hoʻonā mana me ka 0.30 mL o 0.01 mol/L hydrochloric acid VS (ʻaʻole ʻoi aku ma mua o 0.021%).
(3) Sulfate <1.14>—E hana i ka ho'āʻo me 0.6 g o L-Glutamine.E hoʻomākaukau i ka hoʻonā mana me 0.35 mL o 0.005 mol/L sulfuric acid VS (ʻaʻole i ʻoi aku ma mua o 0.028z).
(4) Ammonium <1.02>—E hana i ka ho'āʻo me 0.10 g o L-Glutamine, me ka hoʻohana ʻana i ka distillation ma lalo o ke kaomi hoʻemi ʻia.E hoʻomākaukau i ka hoʻonā hoʻomalu me 10.0 mL o ka Haʻina Ammonium maʻamau.ʻO ka mahana o ka ʻauʻau wai he 45 ℃ (ʻaʻole ʻoi aku ma mua o 0.1%)
(5) Nā Metala Kaumaha <1.07>—E hoʻomau me 1.0 g o L-Glutamine e like me ke ʻano 1, a hana i ka hoʻāʻo.E hoʻomākaukau i ka hoʻonā hoʻomalu me 1.0 mL o ka mea hoʻonā alakaʻi maʻamau (ʻaʻole ʻoi aku ma mua o 10 ppm).
(6) Hao <1.10>—E hoʻomākaukau i ka hopena hoʻāʻo me 1.0 g o L-Glutamine e like me ke ʻano 1, a hana i ka hoʻāʻo e like me ke ʻano A. E hoʻomākaukau i ka hoʻonā mana me 1.0 mL o ka Iron Solution (ʻaʻole ʻoi aku ma mua o 10). ppm)
(7) Nā mea pili—E hoʻoheheʻe i 0.10 g o L-Glutaminein 10 mL o ka wai, a e hoʻohana i kēia hāʻina e like me ka hāpana hāpana.Pipet 1 mL o ka hāpana hāpana, hoʻohui i ka wai e hana pono i 10 mL.Pipet 1 mL o kēia haʻina, e hoʻohui i ka wai e hana pono i 50 mL, a e hoʻohana i kēia haʻina e like me ka hopena maʻamau.E hana i ka ho'āʻo me kēia mau haʻina e like me ka kuhikuhi ʻana ma lalo o Thin-layer Chromatography <2.03>.E kau i 5mL o kēlā me kēia hāpana hāpana a me ka hopena maʻamau ma luna o kahi pā silicagel no ka chromatography ʻāpana lahilahi.A laila e hoʻomohala me ka hui ʻana o ka 1-butanol, ka wai a me ka acetic acid (100) (3:1:1) i kahi mamao o 10 cm, a hoʻomaloʻo i ka pā ma 80 ℃ no 30 mau minuke.E kāpī like i ka hopena o ka ninhydrin i loko o ka hui ʻana o ka methanol a me ka acetic acid (100) (97:3) (1 in 100) ma ka pā, a me ka wela ma 80 ℃ no 10 mau minuke: kahi ʻokoʻa ma waho o ka wahi nui i loaʻa me ka ʻAʻole ʻoi aku ka ikaika o ka hāpana hāpana ma mua o kahi i loaʻa me ka hoʻonā maʻamau.
ʻAʻole ʻoi aku ma mua o 0.3% (1 g, 105℃, 3 hola)
ʻAʻole ʻoi aku ma mua o 0.10%(1 g).
E kaupaona pololei ma kahi o 0.15 g o L-Glutamine, maloʻo mua, e hoʻoheheʻe i 3 mL o ka waikawa formic, e hoʻohui i 50 mL o ka ʻakika acetic (100), a e titrate <2.50> me 0.1 mol/L perchloric acid VS (potentiometric titration).E hana i kahi hoʻoholo hakahaka ma ke ʻano like, a hana i nā hoʻoponopono e pono ai.
ʻO kēlā me kēia mL o 0.1 mol/L perchloric acid VS＝14.61 mg o C5H10N2O3
ʻO nā ipu a me nā pahu mālama—nā ipu paʻa.

Nā Kūlana Kūlana USP <11>-USP Glutamine RS.
ʻIkepili, Hoʻopiʻi Infrared <197K>.
Hoʻololi kikoʻī <781S>: ma waena o +6.3° a me +7.3°, hoʻoholo ʻia ma 20℃
Ho'āʻo - I loko o kahi ipu kūpono, e hoʻomākaukau pono i kahi hopena ma kahi o 40mg no ka ml i ka wai ma kahi o 40 ℃.Hoʻolili a hoʻoheheʻe ʻia me ka wai i ka nui ma mua o ka hoʻohana ʻana.
Nalo ma ka maloo <731>-E hoʻomaloʻo ma 105 ℃ no 3 mau hola;ʻaʻole e emi ma mua o 0.20% o kona kaumaha.
ʻO ke koena ma ka ʻā ʻana <281>ʻAʻole ma mua o 0.30%
Klorida <221>ʻAʻole i ʻoi aku ka nui o ka chloride ma mua o ka 0.50ml o 0.020 N hydrochloric acid: ʻaʻole i ʻoi aku ma mua o 0.05%.
Sulfate <221>ʻAʻole i ʻoi aku ka sulfate ma mua o ka 0.25ml o 0.020 N sulfuric acid: ʻaʻole i ʻoi aku ma mua o 0.03%.
Maemae Chromatographic
Adsorbent: 0.25-mm papa o ka huila silica gel chromatographic.
ʻO ka hopena ho'āʻo: 10mg i ka ml, i ka wai
Hoʻoponopono maʻamau-E hoʻomākaukau i kahi hopena o USP Glutamine RS i loko o ka wai i ʻike ʻia ma kahi o 0.05mg i kēlā me kēia ml.
Ka nui noi: 5μL
Ka Hoʻomohala ʻana i ka Pūnaehana Solvent: kahi hui ʻana o ka waiʻona butyl, ka wai, a me ka waikawa acetic glacial (3:1:1).
Spray Reagent-Dissolve 0.2g ninhydrin i 100ml o ka hui ʻana o ka butyl alcohol a me 2 N acetic acid (95:5)
Ke Kaʻina Hana-E hoʻomau e like me ka kuhikuhi ʻana no ka chromatography layer thin-layer ma lalo o Chromatography <621>, a laila hoʻomaloʻo i ka pā ma 80 ℃ no 30 mau minuke.E kāpīpī i ka pā me ka spray reagent, wela ma 80 ℃ no 10 mau minuke, a nānā ma lalo o ke kukui keʻokeʻo: ʻaʻohe wahi lua o ka chromatogram i loaʻa mai ka hopena hoʻāʻo ʻoi aku ka nui a ʻoi aku ka ikaika ma mua o ka wahi nui o ka chromatogram i loaʻa mai ka solution standrad. (0.5%)
ʻO nā mea haumia kino, Keʻano I <467>: E hoʻokō i nā koi.
Asssy-E hoʻololi ma kahi o 150mg o Glutamine, paona pono, i kahi pahu 125ml, e hoʻoheheʻe i 3ml o ka formic acid a me 50ml o ka glacial acetic acid, a titrate me 0.1 N perchloric acid VS e hoʻoholo i ka hopena potentiometrically.Hana i kahi hoʻoholo hakahaka, a hana i nā hoʻoponopono kūpono (e nānā i ka Titrimetry<541>).ʻO kēlā me kēia ml o 0.1 N perchloric acid ua like ia me 14.615 mg o C5H10N2O3