L-Serine CAS 56-45-1 (H-Ser-OH) Assay 98.5~101.0% Factory High Quality

JP16
ʻO L-Serine, ke hoʻomaloʻo ʻia, ʻaʻole i emi iho ma mua o 98.5% a ʻaʻole ʻoi aku ma mua o 101.0% o C3H7NO3.
Hōʻike ʻia ʻo L-Serine e like me nā keʻokeʻo keʻokeʻo a i ʻole ka pauka crystalline.He ʻono iki kona ʻono.Hiki ke hoʻoheheʻe ʻia i ka wai a me ka waika formic, a hiki ʻole ke hoʻoheheʻe ʻia i ka ethanol (99.5).Hoʻoheheʻe ia i 2 mol/L hydrochloric acid TS.
Identification E hoʻoholo i ke kiko kikoʻī infrared absorption o L-Serine e like me ke kuhikuhi ʻana ma ke ʻano pāpaʻi bromide pāpaʻa ma lalo o Infrared Spectrophotometry<2.25>, a hoʻohālikelike i ke kikoʻī me ka Spectrum Reference: hōʻike nā kikoʻī ʻelua i ka ikaika o ka absorption ma nā helu nalu like.
ʻO ka huli ʻana o ka Optical <2.49> [a]20D: +14.0°~+16.0°(Ma hope o ka maloʻo ʻana, 2.5 g, 2 mol/L hydrochloric acid TS, 25 mL, 100 mm).
pH <2.54> ʻO ka pH o kahi hopena i hoʻomākaukau ʻia ma ka hoʻoheheʻe ʻana i 1.0 g L-Serine i 10 mL o ka wai ma waena o 5.2 a me 6.2.
Maʻemaʻe (1) Maʻemaʻe a me ka waihoʻoluʻu o ka hoʻonā-Dissolve 1.0 g o L-Serine i loko o 10 mL o ka wai: he akaka a kala ʻole ka hopena.
(2) Chloride <1.03>-E hana i ka ho'āʻo me 0.5 g o L-Serine.E hoʻomākaukau i ka hoʻonā mana me 0.30 mL o 0.01mol/L hydrochloric acid VS (ʻaʻole ʻoi aku ma mua o 0.021%)
(3) Sulfate <1.14> Hana i ka ho'āʻo me 0.6 g o L-Serine.E hoʻomākaukau i ka hoʻonā mana me 0.35 mL o 0.005mol/L sulfuric acid VS (ʻaʻole ʻoi aku ma mua o 0.028%).
(4) Ammonium <1.02>-E hana i ka ho'āʻo me 0.25 g o L-Serine.E hoʻomākaukau i ka hoʻonā hoʻomalu me 5.0 mL o ka ʻĀpana Ammonium maʻamau (ʻaʻole ʻoi aku ma mua o 0.02%).
(5) Nā Metala Kaumaha <1.07> E hoʻomau me 2.0 g o L-Serine e like me ke ʻano 1, a hana i ka hoʻāʻo.E hoʻomākaukau i ka hoʻonā hoʻomalu me 2.0 mL o ka mea hoʻonā alakaʻi maʻamau (ʻaʻole ʻoi aku ma mua o 10 ppm).
(6) Hao <1.10> E hoʻomākaukau i ka hopena ho'āʻo me 1.0 g o L-Serine e like me ke ʻano 1, a hana i ka hoʻāʻo e like me ke ʻano A. E hoʻomākaukau i ka hoʻonā mana me ka 1.0mL o ka Iron Solution maʻamau (ʻaʻole ʻoi aku ma mua o 10 ppm) .
(7) Nā mea pili-E hoʻoheheʻe i ka 0.10 g o L-Serine i 10mL o ka wai, a hoʻohana i kēia haʻina e like me ka hāpana hāpana.Pipet 1 mL o ka hāpana hāpana, hoʻohui i ka wai e hana pono i 10 mL.Pipet 1 mL o kēia hāʻina, hoʻohui i ka wai e hana pololei i 50 mL, a hoʻohana i kēia haʻina e like me ka hoʻonā maʻamau.E ʻike i 5mLeachofthesamplesolution a me ka hoʻonā maʻamau ma luna o ka pā silica gel forthin-layer chromatography.A laila e hoʻomohala me ka hui ʻana o 1-butanol, ka wai a me ka ʻakika acetic (100) (3:1:1) i kahi mamao ma kahi o 10 cm, a hoʻomaloʻo i ka pā ma 809C no 30 mau minuke.E kāpīpī i ka wai hoʻonā o ninhydrinin i hui pū ʻia me ka methanol a me ka ʻakika acetic (100) (97:3) (1 i ka 100) ma ka pā, a me ka wela ma 80 ℃ no 10 mau minuke: ʻo kahi ʻē aʻe ma waho o ka wahi nui i loaʻa mai ka hāpana hāpana. ʻoi aku ka ikaika ma mua o ka wahi mai ka hopena maʻamau.
ʻAʻole ʻoi aku ma mua o 0.3% (1 g, 105℃, 3 hola).
ʻAʻole i ʻoi aku ma mua o 0.1%(1 g).
E kaupaona pololei ma kahi o 0.11 g o L-Serine, i maloʻo mua, e hoʻoheheʻe i 3 mL o ka waika formic, e hoʻohui i 50 mL o ka ʻakika acetic (100), a e titrate <2.50> me 0.1 mol/L perchloric acid VS (potentiometric titration).E hana i kahi hoʻoholo hakahaka ma ke ʻano like, a hana i nā hoʻoponopono e pono ai.
ʻO kēlā me kēia mL o 0.1 mol/L perchloric acid VS＝10.51 mg o C3H7NO3
ʻO nā pahu a me ka mālama ʻana i nā pahu pahu-paʻa paʻa.

USP39-NF34
DEFINITION L-Serine loaʻa iā NLT 98.5% a me NMT 101.5% o L-serine (C3H7NO3), i helu ʻia ma ke kumu maloʻo.
IKE
A. KA PALAPALA INFRARED <197K>
HANA
HANA
Laʻana: 100mg o Serine
Pākaʻa: Hoʻohui i 3mL o ka ʻakika formic a me 50mL o ka ʻakika glacial acetic.
Pūnaehana Titrimetric
(E nānā i ka Titrimetry <541>)
Ke ʻano: Hoʻohālikelike pololei
Titrant: 0.1 N perchloric acid VS
ʻImi hope: Potentiometric
Nānā: E hoʻoheheʻe i ka Laʻana ma 3mL o ka waika formic a me 50mL o ka waikawa acetic glacial.Titrate me ka Titrant.
Hana i ka hoʻoholo Blank.
E helu i ka pākēneka o ka serine (C3H7NO3) i ka hāpana i lawe ʻia:
Ka hopena = {[(VS-VB) xNxF]/W} x100
VS= Titrant volume i hoʻopau ʻia e ka Laʻana (mL)
VB= Hoʻopau ʻia ka nui o ka titrant e ka Blank (mL)
N= ka mea maʻamau maoli o ka Titrant (mEq/mL)
F= helu kaulike, 105.1mg/mEq
W= Kaumaha laʻana (mg)
Nā pae hoʻoholo: 98.5% ~ 101.5% ma ke kumu maloʻo
NA mea haumia
KA HOʻOPIʻI KOE <281>: NMT 0.1%
CHLORIDEAND SULFATE, Klorida <221>
Haʻina maʻamau: 0.50mL o 0.020 N waikona hydrochloric
Laʻana: 0.73g o Serine
Nā pae ʻae ʻae: NMT 0.05%
CHLORIDEAND SULFATE, Sulfate <221>
Haʻina maʻamau: 0.10mL o 0.020 N sulfuric acid
Laʻana: 0.33g o Serine
Nā pae ʻae ʻae: NMT 0.03%
HAAWINA <241>: NMT 30ppm
Holoi i kēia mau mea:
HEAVY METALS, Keʻano I <231>: NMT 15ppm (Kumu 1-Jan-201)
NA PUNA PILI
Haʻina kūpono ʻōnaehana: 0.4mg/mL kēlā me kēia o USP L-Serine RS a me USP L-Methionine RS i 0.1 N hydrochloric acid
Haʻina maʻamau: 0.05mg/mL o USP L-Serine RS i loko o 0.1 N hydrochloric acid.[NOTE-He hoʻohāinu koʻikoʻi kēia e like me ka 0.5% o ko ka Haʻina Haʻina.]
Laʻana hoʻonā: 10mg/mL o Serine i 0.1 N hydrochloric acid
Pūnaehana Chromatography E nānā i ka Chromatography <621>, Kromatography Layer Thin-Layer.)
Ke ano: TLC
Adsorbent: 0.25-mm papa o ka huila silica gel chromatographic
Nui noi: 5μL
Ke kūkulu nei i ka ʻōnaehana solvent: Butyl alcohol, glacial acebutyl alcohol a me 2N acetic acid (95:5)
Kūpono ʻōnaehana
Nā koi kūpono: Hōʻike ka chromatogram o ka ʻōnaehana kūpono i nā wahi ʻelua i hoʻokaʻawale ʻia.
Kānāwai
Nā Laʻana: ʻO ka hoʻonā kūpono o ka ʻōnaehana, ka hoʻonā maʻamau, a me ka hopena laʻana.
Ma hope o ka hoʻomaloʻo ʻana i ka ea, e kāpī me ka Spray reagent, a wela ma waena o 100 ° a me 105 ° no 15 min.E nānā i ka pā ma lalo o ke kukui keʻokeʻo.
Paʻa ʻae ʻia: ʻAʻole ʻoi aku ka nui a ʻoi aku ka ikaika o nā wahi lua o ka solution Sample ma mua o ka wahi nui o ka solution Standard.
Nā haumia o kēlā me kēia kanaka: NMT 0.5%
Huina haumia: NMT 2.0%
NA HOAO KUAI
KA HOʻOLOLI OPTICAL, Hoʻololi kikoʻī <781S>
Laʻana hoʻonā: 100mg/mL i ka 2N hydrochloric acid
Nā pae ʻae ʻae: +14.0° a i +15.6°
LOSSON DRYING <731>: Hoʻomaloʻo i kahi hāpana ma 105 ℃ no 3 mau hola: nalowale NMT 0.2% o kona kaumaha.
NA KOI HOU
ACKAGINGAND STORAGE: Mālama ʻia i loko o nā pahu paʻa maikaʻi.
USP REFERENCE STANDARD <11>
USP L-Methionine RS
USP L-Serine RS