L-Tryptophan CAS 73-22-3 (H-Trp-OH) Hōʻike 98.5~101.5% Hale Hana Kiʻekiʻe

L-Tryptophan (H-Trp-OH) (CAS: 73-22-3) AJI 97 Hana Hana
ʻIke: E hoʻohālikelike i ka spectrum absorption infrared o ka hāpana me ke ʻano maʻamau ma ke ʻano pāpaʻi pāpaʻi bromide.
Ka Poʻe Kūikawā [α]20/D: Laʻana Māloʻo, C=1, H2O e hoʻoheheʻe ʻia ma ka hoʻomehana ʻana.
Moku'āina o ka Solution (Transmittance): 0.5g i 20ml o 2 mol/L HCl, spectrophotometer, 430nm, 10mm ka mānoanoa pūnaewele.
Klorida (Cl): 0.7g, A-1, ref: 0.40ml o 0.01mol/L HCl
Ammonium (NH4): B-2
Sulfate (SO4): 1.2g, (1), ref: 0.50ml o 0.005mol/L H2SO4
Hao (Fe): 0.75g, (2), ref: 1.5ml o Iron Std.(0.01mg/ml)
Nā Metala Kaumaha (Pb): 2.0g, (4), ref: 2.0ml o Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (1), ref: 2.0ml o As2O3 Std.
Nā Amino Acids ʻē aʻe: H2O, Hoʻoheheʻe ʻia e ka hoʻomehana ʻana (40 ℃) Laʻana Hoʻāʻo: 50μg, A-6-a, mana: L-Trp 0.25μg
Nalo ma ka maloʻo: ma 105 ℃ no 3 mau hola.
Ke koena ma ka ʻāʻā (Sulfated): AJI Test 13
Ho'āʻo: Lapana maloʻo, 200mg, (1), 3ml o ka ʻakika formic, 50ml o ka ʻakika glacial acetic, 0.1mol/L HCLO4 1ml=20.423mg C11H12N2O2
Ho'āʻo pH: 1.0g i 100ml o H2O

L-Tryptophan (H-Trp-OH) (CAS: 73-22-3) Ke ʻano hoʻāʻo USP35
HOAKAKA
Loaʻa iā Tryptophan ka NLT 98.5% a me ka NMT 101.5% o C11H12N2O2, e like me L-Tryptophan, i helu ʻia ma ke kumu maloʻo.
IKE
A. KA PALAPALA INFRARED <197K>
HANA
HANA
Laʻana hoʻonā: E kau i 200 mg o Tryptophan i loko o kahi pahu 125-mL.E hoʻoheheʻe ʻia i loko o kahi hui ʻana o 3 mL o ka waika formic a me 50 mL o ka waikawa acetic glacial.
Nānā: Titrate me 0.1 N perchloric acid VS, e hoʻoholo ana i ka hopena o ka potentiometric.Hana i kahi hoʻoholo hakahaka, a hana i nā hoʻoponopono kūpono (e nānā i ka Titrimetry <541>).ʻO kēlā me kēia mL o 0.1 N perchloric acid ua like ia me 20.42 mg o C11H12N2O2.
Nā pae hoʻoholo: 98.5% ~ 101.5% ma ke kumu maloʻo
NA mea haumia
Nā mea haumia ʻole
KA HOʻOPIʻI KOE <281>: NMT 0.1%
CHLORIDE A ME SULFATE, Chloride <221>: ʻAʻole hōʻike ʻia kahi ʻāpana 0.73-g ʻoi aku ka chloride ma mua o ka 0.50 mL o 0.020 N waikona hydrochloric (0.05%).[NOTE-E hoʻomehana mālie i ka laʻana hoʻomākaukau e hoʻoheheʻe, inā pono.]
CHLORIDE A ME SULFATE, Sulfate <221>: ʻAʻole hōʻike ʻia kahi ʻāpana 0.33-g i ka sulfate ma mua o ka 0.10 mL o 0.020 N sulfuric acid (0.03%).[NOTE-E hoʻomehana mālie i ka laʻana hoʻomākaukau e hoʻoheheʻe, inā pono.
HAAWINA <241>: NMT 30ppm
NA METAL HEAVY, Hana II <231>: NMT 15ppm
Nā mea haumia kino
HANA 1
Pane A: Trifluoroacetic acid i loko o ka wai (1 mL/L)
Pane B: ʻAka Trifluoroacetic i loko o kahi acetonitrile a me ka wai (80:20) (1 mL/L trifluoroacetic acid solution)
ʻO ka hoʻonā maʻamau: 1.0 mg/L kēlā me kēia o USP Tryptophan Related Compound A RS a me USP Tryptophan Related Compound B RS i loko o ka wai
Laʻana hoʻonā: 10.0 mg/mL o tryptophan i loko o ka wai
ʻO ka hopena kūpono o ka ʻōnaehana: 1.0 mg/L o ka USP Tryptophan Related Compound B RS i ka wai
Māhele paʻalima: E ʻike i ka papa gradient ma lalo.

Pūnaehana Chromatographic (E nānā i ka Chromatography <621>, Pono Pūnaewele.)
Ke ano: LC
ʻIke: UV 220 nm
kolamu: 4.6-mm × 25-cm;5-µm paʻi L1
Ka wela o ke kolamu: 30°
Kahe kahe: 1 mL / min
Ka nui o ka hoʻopio: 20 µL
Kūpono ʻōnaehana
Laʻana: Haʻina kūpono ʻōnaehana
Pono kūpono
Wehewehe maʻamau pili: NMT 5.0%
Kānāwai
Nā Laʻana: Haʻina maʻamau a me ka hāʻina Laʻana
E helu i ka pākēneka o kēlā me kēia haumia i hōʻike ʻole ʻia ma ka ʻāpana o Tryptophan i lawe ʻia:
Ka hopena = (rU/rS) × (CS/CU) × 100
rU = wahi kiʻekiʻe o kēlā me kēia haumia i hōʻike ʻole ʻia i loko o ka hāʻina Laʻana
rS = wahi kiʻekiʻe o tryptophan pili pūhui B ma ka Haʻina maʻamau
CS = ka manaʻo o ka USP Tryptophan Pili pūhui B RS i ka hoʻonā maʻamau (µg/mL)
CU = ka hoʻopaʻa ʻana o Tryptophan i loko o ka hopena Laʻana (µg/mL)
Nā pae ʻae ʻae
Huina paumaele 1: NMT 0.01% o ka huina haumia e hoolele ana ma mua o ka piko tryptophan
Huina haumia 2: NMT 0.03% o ka huina haumia eluting ma hope o ka tryptophan peak.[NĀ MEA-E hoʻokaʻawale i ka piko no ka hui pili tryptophan B.]
ʻO ka pūhui pili tryptophan A: Inā ʻike ʻia kahi kiʻekiʻe o ka pūhui pili tryptophan A i ka hāpana Laʻana, a laila e hoʻokō i ka hoʻāʻo no ke Kaʻina 2: Ka palena o Tryptophan Related Compound A, ma lalo.
KĀNĀINA 2: KA LĀKAI O TRYPTOPHAN PILI A
Pane A: 18 mM monobasic sodium phosphate, kānana a hoʻopau ʻia (pH 2.5), a me ka acetonitrile (9:1)
Pane B: 10 mM monobasic sodium phosphate, kānana a hoʻopau ʻia (pH 2.5), a me ka acetonitrile (1:1)
Pane C: Acetonitrile i ka wai (7:3)
Haʻina maʻamau: 0.1 mg/L o USP Tryptophan Related Compound A RS i loko o ka wai
Laʻana hoʻonā: 10.0 mg/mL o Tryptophan i ka wai
Māhele paʻalima: E ʻike i ka papa gradient ma lalo

Pūnaehana Chromatographic
(E nana Chromatography <621>, Pūnaehana Pono.)
Ke ano: LC
ʻIke: UV 216 nm
kolamu: 3.9-mm × 15-cm;5-µm paʻi L1
Ka wela o ke kolamu: 30°
Kahe kahe: 1 mL / min
Ka nui o ka hoʻopio: 20 µL
Kūpono ʻōnaehana
Laʻana: Haʻina maʻamau
Pono kūpono
Wehewehe maʻamau pili: NMT 5.0%
Kānāwai
Nā Laʻana: Haʻina maʻamau a me ka hāʻina Laʻana
E helu i ka pākēneka o ka pūhui pili tryptophan
A ma ka ʻāpana o Tryptophan i lawe ʻia:
Ka hopena = (rU/rS) × (CS/CU) × 100
rU = wahi kiʻekiʻe o tryptophan pili pūhui A i loko o ka Haʻina Haʻawina
rS = wahi kiʻekiʻe o tryptophan pili pūhui A i loko o ka Haʻina maʻamau
CS = ka manaʻo o ka USP Tryptophan Pūnaʻi Pili A RS i ka hoʻonā maʻamau (µg/mL)
CU = ka hoʻopaʻa ʻana o Tryptophan i loko o ka hopena Laʻana (µg/mL)
Nā pae ʻae ʻae: NMT 10 ppm
NA HOAO KUAI
KA HOOLOLI OPTICAL, Ka Poali Pono <781S>: -29.4° a i ka -32.8°
Laʻana hoʻonā: 10 mg/mL, i ka wai.[NOTE-E hoʻomāmā mālie e hoʻoheheʻe, inā pono.]
PH <791>: 5.5~7.0, ma kahi hoʻonā (1 i ka 100)
LILO MA KA HOOMALOO <731>: E maloo i kahi la'ana ma 105° no 3 mau hola: nalowale NMT 0.3% o kona kaumaha.
NA KOI HOU
PACKAGING a me ka mālama ʻana: mālama i loko o nā pahu paʻa maikaʻi
USP REFERENCE STANDARD <11>
USP L-Tryptophan RS
USP Tryptophan Puhui Pili A RS
3,3′-[Ethylidenebis(1H-indole-1,3-diyl)]bis[2S)-2-aminopropanoic]ʻakika.C24H26N4O4 432.49
USP Tryptophan Puhui Pili B RS
2-Acetamido-3-(1H-indol-3-yl)ʻakika propanoic.C13H14N2O3 246.3