L-(-)-Tyrosine CAS 60-18-4 (H-Tyr-OH) Ho'āʻo 98.5~101.5% Hale Hana Kiʻekiʻe

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) AJI 97 Ka Hana Ho'āʻo
ʻIke: E hoʻohālikelike i ka spectrum absorption infrared o ka hāpana me ke ʻano maʻamau ma ke ʻano pāpaʻi pāpaʻi bromide.
Ka Po'ai Pono [α]20/D: La'ana Māloo, C=5, 1mol/L HCl
Moku'āina o ka Solution (Transmittance): 0.5g i 10ml o 1mol/L HCl, spectrophotometer, 430nm, 10mm ka mānoanoa pūnaewele.
Klorida (Cl): 0.7g, A-1, ref: 0.40ml o 0.01mol/L HCl
Ammonium (NH4): B-2
Sulfate (SO4): 1.2g, (1), ref: 0.50ml o 0.005mol/L H2SO4
Hao (Fe): 1.5g, (2), ref: 1.5ml o Iron Std.(0.01mg/ml)
Nā Metala Kaumaha (Pb): 2.0g, (2), ref: 2.0ml o Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (2), ref: 2.0ml o As2O3 Std.
ʻO nā ʻakika Amino ʻē aʻe: Dilute ammonia R2 Hōʻike Laʻana: 50μg, F-6-a, mana: L-Tyr 0.25μg
Nalo ma ka maloʻo: ma 105 ℃ no 3 mau hola.
Ke koena ma ka ʻāʻā (Sulfated): AJI Test 13
Ho'āʻo: Lapana maloʻo, 180mg, (1), 6ml o ka ʻakika formic, 50ml o ka ʻakika glacial acetic, 0.1mol/L HCLO4 1ml=18.119mg C9H11NO3
Ho'āʻo pH: 0.02g i loko o 100ml saturated aqueous solution

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) USP35 Hana Hana
HOAKAKA
Aia ka Tyrosine i ka NLT 98.5% a me ka NMT 101.5% o L-Tyrosine (C9H11NO3), i helu ʻia ma ke kumu maloʻo.
IKE
A. KA PALAPALA INFRARED <197K>
HANA
HANA
Laʻana: 180 mg o Tyrosine
Pākaʻa: Hoʻohui i 6 mL o ka waika formic a me 50 mL o ka ʻakika acetic glacial.
Pūnaehana Titrimetric
(E nānā i ka Titrimetry <541>)
Ke ʻano: Hoʻohālikelike pololei
Titrant: 0.1 N perchloric acid VS
ʻImi hope: Potentiometric
Nānā: E hoʻoheheʻe i ka Laʻana i 6 mL o ka waika formic a me 50mL o ka ʻakika acetic glacial, a e hoʻoemi me ka Titrant.Hana i ka hoʻoholo Blank.
E helu i ka pākēneka o ka tyrosine (C9H11NO3) ma ka Laʻana i lawe ʻia:
Ka hopena = {[(VS-VB) x N x F]/W} x100
VS = ka nui o ka Titrant i hoʻopau ʻia e ka Laʻana (mL)
VB= ka nui o ka Titrant i hoʻopau ʻia e ka Blank (mL)
N= ka mea maʻamau maoli o ka Titrant (mEq/mL)
F= helu kaulike, 181.2 mg/mEq
W= Kaumaha laʻana (mg)
Nā pae hoʻoholo: 98.5% ~ 101.5% ma ke kumu maloʻo
NA mea haumia
HANA KOE <281>: NMT 0.4%
KLORIDA A ME SULFATE, Klorida <221>
Haʻina maʻamau: 0.20mL o 0.020 N waikona hydrochloric
Laʻana: 0.35 g o Tyrosine.[NOTE-Inā pono, e hoʻoheheʻe i ka Laʻana ma ka hoʻomehana ʻana a kokoke e hoʻolapalapa a hoʻohui i 1 mL o ka waikawa nitric.]
Nā pae ʻae ʻae: NMT 0.04%
KLORIDA A ME KA SULFATE, Sulfate <221>
Haʻina maʻamau: 0.50mL o 0.020 N sulfuric acid
Laʻana: 1.2 g o Tyrosine.[NOTE-Inā pono, e hoʻoheheʻe i ka Laʻana ma ka hoʻohui ʻana i 6 mL o ka waika hydrochloric diluted.]
Nā pae ʻae ʻae: NMT 0.04%
IRON <241>: NMT 30ppm [NOTE-Inā pono, e hoʻohana i 2 mL o ka waikawa hydrochloric e hoʻoheheʻe i ka Laʻana.]
NA METAL KAUHA, Keʻano I <231>: NMT 15ppm
NA PUNA PILI
ʻO ka hopena ammonia i hoʻoheheʻe ʻia: E hoʻoheheʻe i 16 mL o ka ammonia concentrated me ka wai i 100 mL.
ʻO ka hopena kūpono o ka ʻōnaehana: E hoʻoheheʻe i 10 mg o USP L-Tyrosine RS a me 10 mg o USP L-Phenylalanine RS i 1 mL o ka solution ammonia Diluted, a hoʻoheheʻe me ka wai i 25.0 mL.
Haʻina maʻamau: I loko o ka ipu kūpono, e hoʻoheheʻe i ka nui o USP L-Tyrosine RS i 1 mL o ka hoʻoheheʻe ammonia Diluted.E hoʻoheheʻe me ka wai i ka nui e loaʻa ai kahi hopena o 0.05mg/mL.[NOTE-He hoʻopaʻa koʻikoʻi kēia me ka 0.5% o ka hopena Laʻana.]
Laʻana hoʻonā: E hoʻololi i ka 0.1 g o Tyrosine i loko o kahi pahu volumetric 10-mL, e hoʻohui i ka solution ammonia i hoʻoheheʻe ʻia e hoʻoheheʻe ʻia, a hoʻoheheʻe ʻia me ka wai i ka nui.
Pūnaehana Chromatographic (E nānā i ka Chromatography <621>, Thin-Layer Chromatography.)
Ke ano: TLC
Adsorbent: 0.25-mm papa o ka huila silica gel chromatographic
Nui noi: 5μL
Ke hoʻomohala nei i ka ʻōnaehana solvent: Isopropyl alcohol a me ammonium hydroxide (7:3)
Pahu mea hoʻoponopono: 2 mg/mL o ninhydrin i loko o ka hui ʻana o ka butyl alcohol a me 2N acetic acid (95:5)
Kūpono ʻōnaehana
Nā koi kūpono: Hōʻike ka chromatogram o ka ʻōnaehana kūpono i nā wahi ʻelua i hoʻokaʻawale ʻia.
Kānāwai
Nā Laʻana: ʻO ka hoʻonā kūpono o ka ʻōnaehana, ka hoʻonā maʻamau, a me ka hopena laʻana.
E hoʻomaloʻo i ka pā ma waena o 100° a me 105° a nalo loa ka amonia.E kāpīpī me ka Spray reagent, a me ka wela ma waena o 100° a me 105° no 15 min.E nānā i ka pā ma lalo o ke kukui keʻokeʻo.
Paʻa ʻae ʻia: ʻAʻole ʻoi aku ka nui a ʻoi aku ka ikaika o nā wahi lua o ka solution Sample ma mua o ka wahi nui o ka solution Standard.
ʻO nā haumia o kēlā me kēia kanaka: NMT 0.5%
Huina haumia: NMT 2.0%
NA HOAO KUAI
KA HOʻOLOLI OPTICAL, Hoʻololi kikoʻī <781S>
Laʻana hoʻonā: 50 mg/mL i ka 1 N hydrochloric acid
Nā mea e ʻae ai: -9.8° a i -11.2°
LOSSON ON DRYING <731>: Hoʻomaloʻo i kahi hāpana ma 105 ℃ no 3 mau hola: nalowale NMT 0.3% o kona kaumaha.
NA KOI HOU
PACKAGING a me ka mālama ʻana: mālama i loko o nā pahu paʻa maikaʻi.
USP REFERENCE STANDARD <11>
USP L-Phenylalanine RS
USP L-Tyrosine RS