L-Valine CAS 72-18-4 (H-Val-OH) Ho'āʻo 98.5~101.0% Hale Hana Kiʻekiʻe

L-Valine (H-Val-OH) (CAS: 72-18-4) AJI 97 Hana Ho'āʻo
ʻIke: E hoʻohālikelike i ka spectrum absorption infrared o ka hāpana me ke ʻano maʻamau ma ke ʻano pāpaʻi pāpaʻi bromide.
Ka Po'ai Pono [α]20/D: La'ana Māloo, C=8, 6mol/L HCl
Moku'āina o ka Solution (Transmittance): 0.5g i 20ml o H2O, spectrophotometer, 430nm, 10mm ka mānoanoa pūnaewele.
Klorida (Cl): 0.7g, A-1, ref: 0.40ml o 0.01mol/L HCl
Ammonium (NH4): B-2
Sulfate (SO4): 1.2g, (1), ref: 0.50ml o 0.005mol/L H2SO4
Hao (Fe): 1.5g, (2), ref: 1.5ml o Iron Std.(0.01mg/ml)
Nā Metala Kaumaha (Pb): 2.0g, (2), ref: 2.0ml o Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (2), ref: 2.0ml o As2O3 Std.
ʻO nā ʻakika Amino ʻē aʻe: Dilute ammonia R2 Hōʻike Laʻana: 50μg, F-6-a, mana: L-Tyr 0.25μg
Nalo ma ka maloʻo: ma 105 ℃ no 3 mau hola.
Ke koena ma ka ʻāʻā (Sulfated): AJI Test 13
Ho'āʻo: Lapana maloʻo, 120mg, (1), 3ml o ka ʻakika formic, 50ml o ka ʻakika glacial acetic, 0.1mol/L HCLO4 1ml=11.715mg C5H11NO2
Ho'āʻo pH: 1.0g i 20ml o H2O

L-Valine (H-Val-OH) (CAS: 72-18-4)JP16 Hana Hana
ʻO L-Valine, ke maloʻo, ʻaʻole i emi iho ma lalo o 98.5% o C5H11NO2.
Hōʻike ʻia ʻo L-Valine e like me nā keokeo keʻokeʻo a i ʻole ka pauka crystalline.ʻAʻohe ona ʻala a ʻaʻala liʻiliʻi paha, a he ʻono iki kona ʻono, a lilo i ʻawaʻawa.
Hiki ke hoʻoheheʻe ʻia i ka waika formic, hiki ke hoʻoheheʻe ʻia i ka wai, a hiki ʻole ke hoʻoheheʻe ʻia i ka ethanol (95).
Hoʻoheheʻe ia i ka waika hydrochloric dilute.
Identification E hoʻoholo i ke kikoʻī o ka infrared absorption spectrum o L-Valine, i hoʻomaloʻo mua ʻia, e like me ke kuhikuhi ʻia ma ke ʻano pāpaʻi pāpaʻa bromide ma lalo o Infrared Spectrophotometry <2.25>, a e hoʻohālikelike i ke kikoʻī me ka Spectrum Reference: hōʻike nā kikoʻī ʻelua i ka ikaika like o ka absorption ma nā helu nalu like. .
ʻO ka huli ʻana o ka Optical <2.49> [a]20D: +26.5°~+29.0° (ma hope o ka maloʻo ʻana, 2 g, 6 mol/L hydrochloric acid TS, 25 mL, 100 mm).
pH <2.54> E hoʻoheheʻe i ka 0.5 g o L-Valine i 20 mL o ka wai: ʻo ka pH o kēia hopena ma waena o 5.5 a me 6.5.
Maʻemaʻe (1) Maʻemaʻe a me ke kala o ka hopena E hoʻoheheʻe i ka 0.5 g o L-Valine i 20 mL o ka wai: he akaka a kala ʻole ka hopena.
(2) Chloride <1.03>-E hana i ka ho'āʻo me 0.5 g o L-Valine.E hoʻomākaukau i ka hopena hoʻomalu me 0.30 mL o 0.01mol/L hydrochloric acid VS (ʻaʻole ʻoi aku ma mua o 0.021%).
(3) Sulfate <1.14>-E hana i ka ho'āʻo me 0.6 g o L-Valine.E hoʻomākaukau i ka hoʻonā mana me 0.35 mL o 0.005mol/L sulfuric acid VS (ʻaʻole ʻoi aku ma mua o 0.028%).
(4) Ammonium <1.02>-E hana i ka ho'āʻo me 0.25 g o L-Valine.E hoʻomākaukau i ka hoʻonā hoʻomalu me 5.0 mL o ka ʻĀpana Ammonium maʻamau (ʻaʻole ʻoi aku ma mua o 0.02%).
(5) Nā metala kaumaha <1.07> -E hoʻomau me 1.0 g o L-Valine e like me ke ʻano 1, a hana i ka hoʻāʻo.E hoʻomākaukau i ka hoʻonā hoʻomalu me 2.0 mL o ka ʻOi (ʻaʻole ʻoi aku ma mua o 20 ppm).
(6) Arsenic <1.11>-E hoʻomau me ka 1.0g o L-Valine, e hoʻomākaukau i ka hopena hoʻāʻo e like me ke ʻano 2, a hana i ka hoʻāʻo (ʻaʻole ʻoi aku ma mua o 2 ppm).
(7) Nā mea pili-E hoʻoheheʻe i ka 0.10 g o L-Valine i 25mL o ka wai, a hoʻohana i kēia hopena ma ke ʻano he hāpana hāpana.Pipet 1 mL o ka hāpana hāpana, a hoʻohui i ka wai i 50 mL pono.Pipet 5 mL o kēia haʻina, hoʻohui i ka wai e hana pono i 20 mL, a hoʻohana i kēia haʻina e like me ka hopena maʻamau.E hana i ka ho'āʻo me kēia mau haʻina e like me ka kuhikuhi ʻana ma lalo o Thin-layer Chromatography <2.03>.E kau i 5mL o kēlā me kēia hāpana hāpana a me ka hoʻonā maʻamau ma luna o kahi pā silica gel no ka chromatography ʻāpana lahilahi.E hoʻomohala i ka pā me ka hui ʻana o ka 1-butanol, ka wai a me ka acetic acid (100) (3:1:1) i kahi mamao o 10 cm, a hoʻomaloʻo i ka pā ma 80 ℃ no 30 mau minuke.E pāpaʻi like i ka hopena o ka ninhydrin i loko o ka acetone (1 i ka 50) ma ka pā, a me ka wela ma 80 ℃ no 5 mau minuke: ʻaʻole i ʻoi aku ka ikaika o nā kiko ma waho o ka wahi nui o ka hāpana hāpana ma mua o ka wahi mai ka hopena maʻamau.
ʻAʻole i oi aku ma mua o 0.30% (1 g, 105℃,3 hola).
ʻAʻole i ʻoi aku ma mua o 0.1% (1 g).
E kaupaona pololei ma kahi o 0.12 g o L-Valine, i hoʻomaloʻo mua ʻia, a hoʻoheheʻe ʻia i loko o 3 mL o ka waikawa formic, e hoʻohui i 50 mL o ka waikawa acetic (100), a e hoʻoemi <2.50> me 0.1 mol/L perchloric acid VS (potentiometric titration) .Hana i kahi hoʻoholo hakahaka, a hana i nā hoʻoponopono e pono ai.
ʻO kēlā me kēia mL o 0.1 mol/L perchloric acid VS＝11.72 mg o C5H11NO2
ʻO nā pahu a me ka mālama ʻana i nā pahu pahu-paʻa paʻa.