HEPPSO Hydrate CAS 68399-78-0 Purity >99.0% (Titration) Biological Buffer Ultra Pure Grade

Short Description:

Chemical Name: HEPPSO Hydrate

CAS: 68399-78-0

Purity: >99.0% (Titration)

Appearance: White Crystalline Powder

Biological Buffer Ultra Pure Grade

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Description:

Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of HEPPSO Hydrate (CAS: 68399-78-0) with high quality, commercial production. We can provide certificate of analysis (COA), worldwide delivery, small and bulk quantities available, strong after-sale service. Please contact: alvin@ruifuchem.com

Chemical Properties:

Chemical Name	HEPPSO Hydrate
Synonyms	HEPPSO Free Acid; 4-(2-Hydroxyethyl)piperazine-1-(2-Hydroxypropane-3-Sulfonic Acid) Hydrate; N-(Hydroxyethyl)piperazine-N'-2-Hydroxypropanesulfonic Acid; 3-[4-(2-Hydroxyethyl)-1-Piperazinyl]-2-Hydroxypropanesulfonic Acid Hydrate
CAS Number	68399-78-0
CAT Number	RF-PI1677
Stock Status	In Stock, Production Scale Up to Tons
Molecular Formula	C9H20N2O5S·xH2O
Molecular Weight	268.33
Melting Point	158.0~160.0℃
Brand	Ruifu Chemical

Specifications:

Item	Specifications
Appearance	White Crystalline Powder
Purity / Analysis Method	>99.0% (Titration)
Useful pH Range	7.1~8.5
pKa (25°C)	7.5
Water (by Karl Fischer)	<7.00%
Heavy Metals (as Pb)	<5ppm
Solubility (0.1M in H2O)	Clear and Complete
pH (5% in H2O)	7.1~8.5
UV (1M in H2O) A260nm	<0.05 Abs
UV (1M in H2O) A280nm	<0.05 Abs
Chloride (as Cl)	<0.005%
Test Standard	Enterprise Standard
Infrared Spectrum	Conforms to Structure
NMR	Conforms to Structure
Grade	Ultra Pure Grade; Molecular Biology Grade
Usage	Biological Buffer; Zwitterionic Buffer

Package & Storage:

Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement.

Storage Condition: Store in sealed containers at cool and dry place; Protect from light and moisture.

Advantages:

FAQ:

Application:

HEPPSO Hydrate (CAS: 68399-78-0) is a zwitterionic buffer. The working pH range of HEPPSO buffer is 7.1~8.5. HEPPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is commonly used as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPPSO displays no toxicity for crustaceans with which it has been studied and has been deemed suitable for toxicological studies. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPPSO forms a complex with Cu(II) ions so stability constants and concentrations should be taken into consideration when using this buffer in a solution containing copper ions. Though HEPPSO binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.