Adenine CAS 73-24-5 Assay 98.0% ~ 102.0% (Titration) High Purity Factory

Adenine muaj NLT 98.0% thiab NMT 102.0% ntawm C 5H5N5, suav ntawm lub hauv paus qhuav.
IDENTIFICATION
A. INFRARED ABSORPTION <197K>
QHIA
txheej txheem
Qauv: 200 mg ntawm Adenine
Tsis muaj: 80 mL ntawm ib tug sib tov ntawm 100 mL ntawm glacial acetic acid thiab 300 mL ntawm acetic anhydride
Titrimetric System
Hom: Direct titration
Titrant: 0.1 N ib chloric acid VS standardized raws li nram no: Hloov 300 mg ntawm poov tshuaj biphthalate mus rau 150-mL beaker thiab, los ntawm stirring, yaj nyob rau hauv 80 mL ntawm ib tug sib tov ntawm 100 mL ntawm glacial acetic acid thiab 300 mL ntawm acetic anhydride.Titrate nrog chloric acid daws.Txhua 20.42mg ntawm potassium biphthalate yog sib npaug rau 1 mL ntawm 0.1 N perchloric acid.
Endpoint detection: Potentiometric
Kev Ntsuas: Dissolve tus qauv hauv 80 mL ntawm kev sib xyaw ntawm 100 mL ntawm glacial acetic acid thiab 300 mL ntawm acetic anhydride los ntawm nplawm, thiab titrate nrog Titrant.Xam cov feem pua ​​ntawm Adenine (C5H5N5) hauv feem:
Cov txiaj ntsig = [(V − B) × N × F × 100]/W
V = Sample titrant ntim (mL)
B = Qhov ntim titrant (mL)
N = titrant normality (mEq/mL)
F = equivalency factor, 135.13 mg/mEq
W = qhov hnyav ntawm Sample (mg)
Kev lees txais: 98.0% -102.0% ntawm lub hauv paus qhuav
IMPURITIES
Inorganic impurities
RESIDUEON IGNITION <281>: NMT 0.1%
HEAVY METALS, Method II <231>: NMT 10ppm
Organic impurities
txheej txheem
pH 7.0 phosphate buffer: Dissolve 4.54 g ntawm monobasic potassium phosphate hauv dej kom 500 mL ntawm kev daws.Dissolve 4.73 g ntawm anhydrous dibasic sodium phosphate hauv dej kom 500 mL ntawm kev daws.Sib tov 38.9 mL ntawm cov tshuaj monobasic potassium phosphate nrog 61.1 mL ntawm dibasic sodium phosphate tov.Kho, yog tias tsim nyog, los ntawm dropwise ntxiv ntawm dibasic sodium phosphate tov rau pH ntawm 7.0.
Cov txheej txheem kev daws teeb meem: Dissolve ib qho tsim nyog ntawm USP Adenine RS nyob rau hauv dej kub, txias, thiab dilute quantitatively nrog dej kom tau txais ib tug paub concentration ntawm 0.19 mg / mL.
Cov txheej txheem kev daws teeb meem: Pipet 5-mL feem ntawm Standard Tshuag tov rau hauv peb 100-mL volumetric flasks, thiab dilute nrog 0.10 N hydrochloric acid, 0.010 N sodium hydroxide, thiab pH 7.0 phosphate buffer, ntsig txog, rau ntim.
Piv txwv cov tshuaj: Dissolve ib tug tsim nyog kom muaj nuj nqis ntawm Adenine nyob rau hauv dej kub, txias, thiab dilute quantitatively nrog dej kom tau ib tug paub txog concentration ntawm 0.19 mg / mL.
Piv txwv cov tshuaj: Dissolve ib tug tsim nyog kom muaj nuj nqis ntawm Adenine nyob rau hauv dej kub, txias, thiab dilute quantitatively nrog dej kom tau ib tug paub txog concentration ntawm 0.19 mg / mL.
Cov qauv kev daws teeb meem: Pipet 5-mL feem ntawm cov qauv Tshuag tshuaj rau hauv peb 100-mL volumetric flasks, thiab dilute nrog 0.10 N hydrochloric acid, 0.010 N sodium hydroxide, thiab pH 7.0 phosphate buffer, feem, rau ntim.
Spectrometric tej yam kev mob
(Saib Spectrophotometry thiab Lub Teeb-Scattering <851>.)
Mode: UV-Vis
Lub wavelength: 220-320nm
Cell: 1 cm
Dawb: Dej
Kev tsom xam
Cov Qauv: Cov qauv kev daws teeb meem thiab cov qauv kev daws teeb meem
Kev lees txais cov txheej txheem: Cov kev nqus nqus, suav ntawm lub hauv paus qhuav, ntawm lub wavelengths ntawm qhov siab tshaj plaws absorbance, rau txhua khub ntawm cov kev daws teeb meem tsis txawv ntau dua 2.0%.
TSEEM CEEB
LOSSON DRYING <731>: Qhuav ib qho qauv ntawm 110 ° rau 4 h: nws poob NMT 1.0% ntawm nws qhov hnyav.
NITROGEN CONTENT, Txoj Kev II 〈461〉: 50.2% - 53.4%, suav ntawm lub hauv paus qhuav
TSEEM CEEB
PACKAGING THIAB STORAGE: Khaws rau hauv cov thawv kaw kom zoo.
USP REFERENCE STANDARDS <11>
USP Adenine RS