Chenodeoxycholic Acid (CDCA) CAS 474-25-9 Assay ≥98% (Dry Basic)

Chenodeoxycholic Acid
C24H40O4: 392.57
3a,7a-Dihydroxy-5b-cholan-24-oic acid [474-25-9]
Chenodeoxycholic Acid, thaum qhuav, muaj tsis tsawg dua 98.0z thiab tsis ntau tshaj 101.0% ntawm C24H40O4.
Kev piav qhia Chenodeoxycholic Acid tshwm sim dawb, muaju, crystalline hmoov lossis hmoov.
Nws yog dawb do soluble nyob rau hauv methanol thiab ethanol (99.5), soluble nyob rau hauv acetone, thiab xyaum insoluble nyob rau hauv dej.
Kev txheeb xyuas Kev txheeb xyuas qhov kev nqus ntawm infrared spectrum ntawm Chenodeoxycholic Acid, yav tas los qhuav, raws li qhia nyob rau hauv cov poov tshuaj bromide disk txoj kev nyob rau hauv Infrared Spectrophotometry <2.25>, thiab muab piv cov spectrum nrog rau Reference Spectrum: ob qho tib si spectra pom zoo sib xws ntawm kev nqus ntawm tib yoj tus lej.
Optical rotation <2.49> [a]20D : +11.0 ° ~ +13.0 ° (tom qab ziab, 0.4 g, ethanol (99.5), 20 mL, 100 mm).
Melting point <2.60> 164 ~ 169 ℃
Purity (1) Chloride <1.03>- Dissolve 0.36 g ntawm Chenodeoxycholic Acid nyob rau hauv 30 mL ntawm methanol, ntxiv 10 mL ntawm dilute nitric acid thiab dej kom 50 mL, thiab ua qhov kev sim nrog cov tshuaj no.Npaj cov tshuaj raws li nram no: Rau 1.0 mL ntawm 0.01 mol / L hydrochloric acid VS ntxiv 30 mL ntawm methanol, 10 mL ntawm dilute nitric acid thiab dej kom 50 mL (tsis ntau tshaj 0.1%).
(2) Cov hlau hnyav <1.07>-Tau nrog 1.0 g ntawm Chenodeoxycholic Acid raws li Txoj Kev 4, thiab ua qhov kev sim.Npaj cov tshuaj tswj nrog 2.0 mL ntawm Standard Lead Solution (tsis ntau tshaj 20 ppm).
(3) Barium-Rau 2.0 g ntawm Chenodeoxycholic Acid ntxiv 100 mL dej, thiab boil rau 2 feeb.Rau cov tshuaj no ntxiv 2 mL ntawm hydrochloric acid, boil rau 2 feeb, lim tom qab txias, thiab ntxuav lub lim nrog dej kom txog thaum tau txais 100 mL ntawm cov lim.Rau 10 mL ntawm filtrate ntxiv 1 mL ntawm dilute sulfuric acid: tsis muaj turbidity tshwm sim.
(4) Lwm yam tshuaj-Dissolve 0.20 g ntawm Chenodeoxycholic Acid nyob rau hauv ib tug sib tov ntawm acetone thiab dej (9:1) kom meej meej 10 mL, thiab siv cov tshuaj no raws li cov qauv tov.Cais, yaj 10 mg ntawm lithocholic acid rau nyias-txheej chromatography nyob rau hauv lub sib tov ntawm acetone thiab dej (9: 1) kom meej meej 10 mL.Pipet 2 mL ntawm cov tshuaj no, ntxiv cov sib tov ntawm acetone thiab dej (9: 1) ua kom raws nraim 100 mL, thiab siv cov tshuaj no raws li tus qauv tov (1).Cais, yaj 10 mg ntawm ursodeoxycholic acid nyob rau hauv qhov sib tov ntawm acetone thiab dej (9: 1) ua kom raws nraim 100 mL, thiab siv cov tshuaj no raws li tus qauv tov (2).Cais, yaj 10 mg ntawm cholic acid rau nyias-txheej chromatography nyob rau hauv cov sib tov ntawm acetone thiab dej (9: 1) ua kom raws nraim 100 mL, thiab siv cov tshuaj no raws li tus qauv tov (3).Pipet 1 mL ntawm cov qauv tov, thiab ntxiv cov sib tov ntawm acetone thiab dej (9: 1) ua kom raws nraim 20 mL.Pipet 0.5 mL, 1 mL, 2 mL, 3 mL thiab 5 mL ntawm cov tshuaj no, ntxiv cov sib tov ntawm acetone thiab dej (1) rau lawv txhua tus kom ua tau raws nraim 50 mL, thiab xaiv cov kev daws teeb meem no raws li cov qauv kev daws teeb meem A. B, txheem tov C, txheem tov D thiab txheem tov E, feem.Ua qhov kev sim nrog cov kev daws teeb meem no raws li qhia hauv Thin-txheej Chromatography<2.03>.Spot 5 mL txhua tus qauv kev daws teeb meem, cov qauv kev daws teeb meem (1), (2), (3) thiab cov qauv kev daws teeb meem A, B, C, D thiab E ntawm lub phaj ntawm silica gel rau nyias txheej chromatography.Tsim lub phaj nrog sib tov ntawm 4-methyl-2-pentanone, toluene thiab formic acid (16: 6: 1) mus rau qhov deb li ntawm 15 cm, airdry lub phaj, thiab ntxiv qhuav ntawm 1209C rau 30 feeb.Tam sim ntawd, tshuaj tsuag tusyees ntawm phosphomolybdic acid n-hydrate nyob rau hauv ethanol (95) (1 nyob rau hauv 5) ntawm lub phaj, thiab kub ntawm 120 ℃ rau 2 mus rau 3 feeb: qhov chaw sib xws rau qhov chaw nrog cov txheej txheem tov (1) tsis txhob khaus ntau dua li qhov chaw nrog cov qauv kev daws teeb meem (1), qhov chaw sib txuam nrog qhov chaw nrog cov qauv kev daws teeb meem (2) tsis yog khaus ntau dua li qhov chaw nrog cov txheej txheem daws teeb meem (2), thiab qhov chaw sib haum rau qhov chaw. nrog cov txheej txheem kev daws teeb meem (3) tsis yog khaus ntau dua li qhov chaw nrog cov txheej txheem daws teeb meem
(3).Raws li muab piv rau cov chaw nrog cov qauv kev daws teeb meem A, B, C, D thiab E, qhov chaw uas tsis yog qhov chaw tseem ceeb thiab lwm yam tshaj li cov chaw uas tau hais los saum toj no tsis yog khaus tshaj qhov chaw nrog cov txheej txheem daws E, thiab tag nrho cov nyiaj ntawm lawv tsis ntau tshaj 1.5%.
Poob ntawm ziab <2.41> Tsis ntau tshaj 1.5% (1 g, 105 ℃, 3 teev).
Residue ntawm ignition <2.44> Tsis ntau tshaj 0.1% (1 g).
Kev ntsuas ntsuas qhov tseeb txog 0.5 g ntawm Chenodeoxycholic Acid, yav tas los qhuav, yaj hauv 40 mL ntawm ethanol (95) thiab 20 mL dej, thiab titrate <2.50> nrog 0.1 mol / L sodium hydroxide VS (potentiometric titration).Ua ib qho kev txiav txim siab dawb paug ib yam nkaus, thiab ua kom muaj kev kho qhov tsim nyog.
Txhua mL ntawm 0.1 mol / L sodium hydroxide VS ＝ 39.26 mg ntawm C24H40O4
Containers thiab cia Containers-Tight containers.