D-Leucine CAS 328-38-1 HD-Leu-OH Assay 98.5 ~ 101.5% Hoobkas Zoo

pH
Noj 0.50g ntawm cov khoom no, ntxiv 50ml dej, cua sov kom yaj, txias, thiab txiav txim raws li txoj cai (General 0631), pH tus nqi yuav tsum yog 5.5 ~ 6.5.
Transmittance ntawm kev daws teeb meem
Noj 0.50g ntawm cov khoom no, ntxiv 50ml dej, cua sov kom yaj, txias, ultraviolet-pom pom spectrophotometry (Txoj cai 0401), ntsuas qhov transmittance ntawm 430nm wavelength, tsis tsawg tshaj li 98.0%.
Chloride
Noj 0.25g ntawm cov khoom no thiab xyuas nws raws li txoj cai (Txoj cai 0801).Piv nrog rau kev tswj cov tshuaj ua los ntawm 5.0 ml ntawm standard sodium chloride tov, nws yuav tsum tsis txhob muaj ntau concentrated (0.02%).
Sulfate
Noj 1.0g ntawm cov khoom no thiab xyuas nws raws li txoj cai (Txoj cai 0802).Piv nrog rau kev tswj kev daws teeb meem ua los ntawm 2.0 ml ntawm cov txheej txheem potassium sulfate tov, nws yuav tsum tsis txhob muaj ntau concentrated (0.02%).
Ammonium ntsev
Noj cov khoom no 0.10g, xyuas raws li txoj cai (Txoj cai lij choj 0808), thiab sib piv nrog kev tswj cov tshuaj ua los ntawm cov txheej txheem ammonium chloride tov 2.0 ML, tsis ntxaum (0.02%).
Lwm yam Amino Acid
Siv cov khoom tsim nyog ntawm cov khoom no, ntxiv dej kom yaj thiab dilute los ua cov tshuaj uas muaj txog 20mg ib lml raws li kev sim tshuaj;Siv 1ml rau kev ntsuas qhov tseeb thiab muab tso rau hauv 200ml ntsuas lub khob, dilute mus rau qhov ntsuas nrog dej, co, raws li kev tswj kev daws teeb meem;Siv tus nqi tsim nyog ntawm leucine siv thiab valine siv, thiab muab tso rau hauv tib lub khob ntsuas, dej tau ntxiv rau yaj thiab diluted los npaj cov tshuaj uas muaj txog 0.4mg txhua ib 1ml raws li kev daws teeb meem.Raws li cov txheej txheem nyias chromatography (General 0502), nqus cov saum toj no peb cov kev daws teeb meem txhua 5 u1, raws li, ntawm tib silica gel G nyias txheej phaj, nrog n-butanol-dej-glacial acetic acid (3: 1: 1) rau txoj kev loj hlob, tom qab xa mus, huab cua kom qhuav, txau nrog ninhydrin hauv cov tshuaj acetone (1-50), cua sov ntawm 80 ° C kom txog thaum cov pob tshwm, thiab kuaj tam sim ntawd.Kev tswj kev daws teeb meem yuav tsum pom qhov pom tseeb, thiab cov txheej txheem siv tau yuav tsum pom ob qhov sib cais tag nrho.Yog tias cov tshuaj ntsuam xyuas qhia pom qhov tsis huv, cov xim yuav tsum tsis txhob sib sib zog nqus (0.5%) tshaj qhov chaw tseem ceeb ntawm kev tswj cov tshuaj.
Poob rau ziab
Noj cov khoom no, qhuav ntawm 105 ℃ rau 3 teev, poob ntawm qhov hnyav yuav tsum tsis pub tshaj 0.2% (Txoj cai 0831).
Residue ntawm Ignition
Noj 1.0g ntawm cov khoom no thiab xyuas nws raws li txoj cai (Txoj cai 0841).Cov seem seem yuav tsum tsis pub tshaj 0.1%.
Hlau ntsev
Noj 1.5g ntawm cov khoom no thiab xyuas nws raws li txoj cai (Txoj cai 0807).Piv nrog rau kev tswj kev daws teeb meem ua los ntawm 0.001% ntawm cov qauv hlau tov, nws yuav tsum tsis txhob sib sib zog nqus ().
Hnyav Hlau
Cov seem tshuav nyob rau hauv cov khoom ntawm kev noj cov khoom siv hluav taws xob yuav tsum tsis txhob muaj ntau tshaj 10 feem ntawm ib lab ntawm cov hlau hnyav thaum kuaj xyuas los ntawm txoj cai lij choj (General Principles 0821, Law II).
Arsenic ntsev
Ntxiv 5ml dej, 1ml sulfuric acid thiab 10ml sulfurous acid, tshav kub mus rau ib lub ntim ntawm txog 2ml nyob rau hauv ib da dej, ntxiv 5ml dej, ntxiv Dropwise ammonia tov kom txog rau thaum qhov taw qhia ntawm phenolphthalein yog nruab nrab, ntxiv hydrochloric acid 5ml, ntxiv dej kom 28ml. , raws li kev soj ntsuam kev cai lij choj (General Principles 0822 Thawj txoj cai), yuav tsum ua raws li cov kev cai (0.0001%).
Cov kab mob endotoxin
Noj cov khoom no, xyuas raws li txoj cai (General 1143), txhua lg leucine uas muaj endotoxin yuav tsum tsawg dua 25EU.(rau txhaj tshuaj)
328-38-1 - Kev Txiav Txim Cov Ntsiab Lus
Noj cov khoom no txog 0.1g, precision hnyav, ntxiv anhydrous formic acid 1 ml yaj, ntxiv glacial acetic acid 25ml, raws li txoj cai potentiometric titration (General txoj cai 0701), nrog perchloric acid titration tov (O. 1 mol / L) titration , thiab cov txiaj ntsig ntawm titration tau raug kho nrog kev kuaj dawb.Txhua 1 ml ntawm perchloric acid titration tov (0.1 mol / L) sib raug rau 13.12 mg ntawm C6H13N02.