D-Tryptophan CAS 153-94-6 (HD-Trp-OH) Assay 98.5 ~ 101.0% Hoobkas

Acidity
Noj 1g ntawm cov khoom no, ntxiv 50ml dej kom yaj, thiab ntsuas raws li txoj cai (Txoj Cai 5.4).Tus nqi pH yuav tsum yog 5.4 ~ 6.4.
Transmittance ntawm kev daws
noj 0.5g ntawm cov khoom no, ntxiv 2mol / L hydrochloric acid tov 20ml kom yaj, raws li ultraviolet-pom spectrophotometry (Txoj cai 0401), ntsuas qhov transmittance ntawm 430nm wavelength, tsis tsawg tshaj li 95.0%.
Chloride
Noj 0.25g ntawm cov khoom no thiab xyuas nws raws li txoj cai (Txoj cai 0801).Piv nrog rau kev tswj cov tshuaj ua los ntawm 0.02% ntawm tus qauv sodium chloride tov, nws yuav tsum tsis txhob muaj ntau concentrated ().
Sulfate
Noj l.0g ntawm cov khoom no thiab xyuas nws raws li txoj cai (Txoj cai 0802).Piv nrog rau kev tswj kev daws teeb meem ua los ntawm 0.02% ntawm cov txheej txheem potassium sulfate tov, nws yuav tsum tsis txhob muaj ntau concentrated ().
Ammonium ntsev
Noj 0.10g ntawm cov khoom no thiab xyuas nws raws li txoj cai (Txoj cai 0808).Piv nrog rau kev tswj cov tshuaj ua los ntawm 0.02% ntawm tus qauv ammonium chloride tov, nws yuav tsum tsis txhob tob ().
Lwm yam Amino Acids
Noj 0.30g ntawm cov khoom no, muab tso rau hauv 20ml ntsuas lub hwj, ntxiv 1 mol / L hydrochloric acid tov 1 ml thiab dej kom yaj, dilute nrog dej mus rau lub teev, co zoo, raws li ib tug tshuaj ntsuam xyuas;noj 1 ml kom raug, muab tso rau hauv 200ml ntsuas lub raj mis, dilute nws mus rau nplai nrog dej, co zoo, thiab siv los tswj kev daws teeb meem.Noj 10mg ntawm tryptophan tswj thiab tyrosine tswj, thiab muab tso rau hauv tib lub 25ml ntsuas fwj, 1 ml ntawm 1 mol / L hydrochloric acid tov thiab ib tug tsim nyog tus nqi ntawm cov dej ntxiv rau yaj cov tshuaj.Cov tshuaj yog diluted mus rau lub teev nrog dej thiab ces shaken los tsim ib tug haum tov rau lub system.Raws li cov txheej txheem nyias chromatography (General 0502), nqus cov saum toj no peb cov kev daws teeb meem txhua 2 μL, feem, ntawm tib silica gel G nyias txheej phaj, nrog n-butanol-glacial acetic acid-dej (3: 1: 1) kom nthuav tawm, nthuav tawm, qhuav, tshuaj tsuag nrog ninhydrin hauv cov tshuaj acetone (1-50), cua sov ntawm 80 ℃ kom txog thaum lub * taw tes tshwm, thiab saib tam sim ntawd.Kev tswj kev daws teeb meem yuav tsum pom qhov pom tseeb, thiab cov txheej txheem siv tau yuav tsum pom ob qhov sib cais tag nrho.Yog tias cov tshuaj ntsuam xyuas qhia pom qhov tsis huv, cov xim yuav tsum tsis txhob sib sib zog nqus (0.5%) tshaj qhov chaw tseem ceeb ntawm kev tswj cov tshuaj.
Poob rau ziab
Noj cov khoom no, qhuav ntawm 105 ℃ rau 3 teev, poob ntawm qhov hnyav yuav tsum tsis pub tshaj 0.2% (Txoj cai 0831).
Residue ntawm Ignition
Noj 1.0g ntawm cov khoom no thiab xyuas nws raws li txoj cai (Txoj cai 0841).Cov seem seem yuav tsum tsis pub tshaj 0.1%.
Hlau ntsev
Noj cov khoom no 1.0g tom qab hlawv thiab ashing, ntxiv hydrochloric acid (2ml) rau qhov seem, qhuav rau hauv ib da dej, ces ntxiv dilute hydrochloric acid (4ml), yaj nyob rau hauv me ntsis tshav kub, ntxiv dej (30ml) thiab ammonium persulfate. (50mg), thiab xyuas nws raws li txoj cai (Txoj cai 0807), nws yuav tsum tsis txhob tob (0.002%) tshaj li kev tswj cov tshuaj ua los ntawm cov txheej txheem hlau.
Hnyav Hlau
Cov seem tshuav nyob rau hauv cov khoom ntawm kev noj cov khoom siv hluav taws xob yuav tsum tsis txhob muaj ntau tshaj 10 feem ntawm ib lab ntawm cov hlau hnyav thaum kuaj xyuas los ntawm txoj cai lij choj (General Principles 0821, Law II).
Arsenic ntsev
noj 2.0g ntawm cov khoom no, ntxiv 5ml ntawm hydrochloric acid thiab 23ml dej kom yaj, xyuas raws li txoj cai (Txoj cai 0822 thawj txoj cai), yuav tsum ua raws li cov kev cai (0.0001%).
Cov kab mob endotoxin
noj cov khoom no, ntxiv tib neeg endotoxin kuaj dej, thiab cua sov rau 80 ℃ kom yaj nws, xyuas raws li txoj cai (General 1143), txhua lg ntawm tryptophan uas muaj endotoxin yuav tsum tsawg dua 50EU.(rau txhaj tshuaj)
Kev txiav txim siab txog cov ntsiab lus
Noj cov khoom no txog 0.15g, precision hnyav, ntxiv anhydrous formic acid 3ml yaj, ntxiv glacial acetic acid 50ml, raws li txoj cai muaj peev xwm titration (Txoj cai 0701), nrog hydrochloric acid titration tov (0.1 mol / L) titration, thiab cov Cov txiaj ntsig ntawm titration tau raug kho nrog kev kuaj dawb.Txhua 1 ml ntawm perchloric acid titration tov (0.1 mol / L) sib raug rau 20.42mg ntawm C11H12N2O2.