EDCHCl CAS 25952-53-8 Coupling Reagent Purity > 99.0% (T) Hoobkas

1-(3-Dimethylaminopropyl)-3-Ethylcarbodiimide Hydrochloride (EDC·HCl) (CAS: 25952-53-8) yog dawb crystalline hmoov, yooj yim deliquescent, soluble nyob rau hauv dej, soluble nyob rau hauv ethanol, siv raws li ib tug activating reagent rau carboxyl pab pawg nyob rau hauv amide synthesis, kuj siv rau activating phosphate pawg, cross-linking ntawm proteins thiab nucleic acids, thiab zus tau tej cov immunocuplers.EDC-HCl yog siv nyob rau hauv pH ntau ntawm 4.0-6.0 thiab feem ntau yog siv nrog N-Hydroxysuccinimide (NHS) los yog N-Hydroxysulfosuccinimide sodium ntsev los txhim kho kev coupling efficiency.
EDC · HCl yog dej-soluble carbodiimide, dav siv rau peptide coupling.EDC · HCl yog siv los ua carboxyl activating agent.Nws ua lub luag haujlwm tseem ceeb rau immobilization ntawm cov biomolecules loj hauv kev koom nrog N-hydroxysuccinimide.Nws kuj tseem siv rau hauv acylation ntawm phosphorane.
Cov dej-soluble derivative ntawm carbodiimide pab tau rau conjugating haptens rau cov proteins thiab polypeptides.Siv los hloov kho NMDA receptors thiab ua tus neeg sawv cev condensing hauv peptide synthesis.Qhov zoo ntawm EDAC coupling yog qhov yooj yim tshem tawm ntawm cov reagent ntau dhau thiab sib xws urea los ntawm kev ntxuav nrog dilute acid lossis dej.Carbodiimides catalyze tsim cov amide bonds, carboxylic acids, thiab amines los ntawm activating lub carboxylate los ua O-acylurea.Qhov nruab nrab no tuaj yeem tawm tsam los ntawm amine ncaj qha los tsim ib qho amide.EDAC tau tso tawm raws li cov kua dej urea derivative.
EDC · HCl tuaj yeem siv rau kev sib txuas ntawm amides.Nws yog siv los ua tus neeg sawv cev sib txuas hauv kev sib txuas ntawm esters los ntawm carboxylic acids siv dimethylaminopyridine ua cov catalyst.
Muaj peb feem ntau siv condensing agents: DCC, DIC thiab EDC · HCl.Kev siv hom condensing tus neeg sawv cev no feem ntau xav tau qhov sib ntxiv ntawm acylation catalysts lossis activators, xws li DMAP, HOBt, thiab lwm yam. Vim tias qhov sib ntxiv nruab nrab ntawm cov kua qaub rau carbodiimide hauv thawj theem ntawm cov tshuaj tiv thaiv tsis ruaj khov, yog tias acylation catalyst tsis yog siv los hloov nws mus rau hauv cov active ester los yog active amide, nws yuav tsum rearranged rau hauv cov khoom ruaj khov los ntawm urea (Path B).Condensation activator: Muaj cov nram qab no hom kev siv condensation activator.Tam sim no, 4-N, N-dimethylpyridine (DMAP) tau siv dav los ua kom muaj ntau yam kev cuam tshuam acylation.Qee zaum 4-PPY tuaj yeem siv tau thaum cov nyhuv catalytic ntawm DMAP tsis zoo.Raws li cov ntaub ntawv cuam tshuam, nws lub peev xwm catalytic yog li ntawm ib txhiab npaug siab dua DMAP.Ntawm peb cov condensers feem ntau, DCC thiab DIC yog pheej yig dua.Feem ntau DCC thiab DMAP tau siv ua ke.Ib qho tsis zoo ntawm kev siv DCC yog tias lwm yam khoom ntawm cov tshuaj tiv thaiv, dicyclohexylurea, muaj me me solubility nyob rau hauv cov organic theem tab sis yog me ntsis soluble, yog li nws yog ib qho nyuaj rau tshem tawm nws kom huv si los ntawm ib co feem ntau siv purification txoj kev, recrystallization, kab chromatography, thiab lwm yam;vim hais tias cov solubility ntawm dicyclohexylurea nyob rau hauv ether yog tsawg dua lwm cov kuab tshuaj, yog li ntawd, kev kho mob ntawm cov tshuaj tiv thaiv no feem ntau evaporates cov tshuaj tiv thaiv hnyav thiab ntxiv ether, thiab lim tawm feem ntau ntawm dicyclohexylurea ua ntej kev kho mob ntxiv.Vim hais tias cov diisopropylurea ua los ntawm DIC muaj zoo solubility nyob rau hauv cov organic solvents, feem ntau yog siv nyob rau hauv combinatorial chemistry solid-phase synthesis.EDCI tam sim no siv feem ntau hauv tshuaj chemistry.Ib qho ntawm nws cov yam ntxwv tseem ceeb yog tias urea tsim tom qab cov tshuaj tiv thaiv yog dej-soluble thiab yooj yim los ntxuav.Feem ntau, EDCI tau ua ke nrog HOBt (ceeb toom: qhov tshuaj tiv thaiv HOBt no feem ntau yog qhov tsis tseem ceeb, txwv tsis pub nws yuav ua rau qis qis qis).Qee lub sij hawm yog tias txoj hauj lwm hindrance ntawm cov kua qaub yog loj los yog muaj ib pab pawg neeg tshem tawm electron, cov tshuaj tiv thaiv yuav nyob twj ywm rau hauv ester active kauj ruam (lub active ester muaj ib tug muaj zog spectrum teeb liab thiab yuav ntes tau los ntawm MS los yog LC-MS).