Fmoc-Asp(OtBu)-OH CAS 71989-14-5 Purity > 99.0% (HPLC) Hoobkas

Fmoc-L-Aspartic Acid β-tert-Butyl Ester (Fmoc-Asp(OtBu)-OH) (CAS: 71989-14-5) yog ib qho tshuaj nruab nrab, feem ntau siv hauv cov txheej txheem polypeptide synthesis, rau kev tiv thaiv amino acid.Fmoc-amino acids, siv nyob rau hauv peptide synthesis, siv los ua cov organic synthesis intermediate, tshuaj intermediate, biochemical reagent lossis tshuaj reagent.
Kev npaj: Ntxiv 581g ntawm tert-Butyl Acetate thiab 133g ntawm Aspartic Acid rau ML peb lub qhov ncauj lub raj mis, do, thiab ntxiv perchloric acid dropwise, cov tshuaj tiv thaiv ntawm 10 ℃ rau 48 teev, txias rau 0 ℃, ces ntxiv 700ml dej, rho tawm (qhov seem tert-Butyl Acetate li hnyav extraction), neutralized nrog Na2CO3 rau pH = 7, kua sib cais, tert-Butyl Acetate txheej yog concentrated nyob rau hauv txo siab kom tau ib tug sib tov ntawm Asp (OtBu) 2, Asp (OtBu) thiab Asp-OtBu roj, uas tau ua ke nrog cov txheej txheem aqueous siv.Cov txiaj ntsig sib xyaw tau raug xa mus rau 125 ml peb lub raj mis, G cuso4 · 5H2O tau ntxiv, thiab pH raug kho rau 3 nrog cov concentrated hydrochloric acid.Cov sib tov yog stirred thiab rhuab mus rau 50 ℃.Rau 12 teev los npaj Cu[Asp(OtBu)]x.(x = 1~2);Tom qab ntawd mus rau chav sov, ntxiv 116.2G tetramethylethylenediamine thiab 300ml dioxane, thiab tom qab ntawd kho cov pH rau 8 ~ 9 nrog triethylamine los npaj Asp (OtBu);Tom qab ntawd ntxiv 82gFmoc-OSu rau hauv Asp (OtBu), tswj pH 8 ~ 9, hnov ​​​​mob rau 8 teev, ces rho tawm nrog ethyl acetate, acidify, ntxuav nrog dej, nqus distillation, crystallization, ziab, 85g ntawm Fmoc-L-aspartic acid beta -tert-butyl ester tau txais los ntawm 20.7%.Los ntawm HPLC tsom xam ntawm Fmoc-L-Aspartic Acid β-tert-Butyl Ester.