H-Glu(OEt)-OEt · HCl CAS 1118-89-4 L-Glutamic Acid Diethyl Ester Hydrochloride Purity > 99.0% (HPLC) Hoobkas

I. Cov txheej txheem tshuaj xyuas
1. Melting point
Cov cuab yeej digital melting point yog siv
Kauj Ruam 2: Cov ntsiab lus
2.1 Reagents
Kauj Ruam 1: Formic acid
2. Glacial acetic acid
3. Perchloric acid txheem titration tov (C = 0.1000mol / l)
4. Crystal Violet qhia kua: 5g/l.
2.2 Cov cuab yeej thiab cov cuab yeej siv
1. Beaker 150ml
2. Microburet: 10ml
2. 3 Cov kauj ruam tsom xam
2. 3. Kuv hnyav txog 300mg ntawm cov qauv ua ntej qhuav ntawm 105 ℃ rau 3h, muab tso rau hauv 150ml Bethel, ntxiv 3ml ntawm formic acid, ntxiv 50ml ntawm ice acetic acid, ntxiv 2 tee ntawm crystal violet test tov ( TS-74), thiab titrate nrog 0.1mol / L perchloric acid kom txog thaum ntsuab kawg taw tes lossis kom txog thaum lub xiav ploj tag.
2. 3. 2 Kev suav txheej txheem
X yog sib npaug rau C (V1-V2) x 0.2042 / M x 100
C yog qhov concentration ntawm perchloric acid standard titration tov, qhia nyob rau hauv moles ib liter (mol / l).
V1 yog cov ntim ntim ntawm perchloric acid tus qauv titration concentrated tov hauv cov qauv titration, qhia hauv ml (ml).
Cov ntim tau noj ntawm perchloric acid standard titration tov nyob rau hauv V2 dawb paug xeem, qhia nyob rau hauv ml (ml)
0.2042 thiab 1.00ml perchloric acid tus qauv titrated concentrated kua theem thaum qhov zoo ntawm cov khoom no qhia nyob rau hauv G
M Qhov zoo ntawm cov qauv, qhia hauv grams (g).
2. 4 Kev Kho
Yog hais tias chav tsev kub qhov sib txawv ntawm cov titration test tov thiab calibration perchloric acid standard tov tshaj 10 ℃, nws yuav tsum tau rov calibrated;Txwv tsis pub, qhov concentration C1 ntawm perchloric acid txheem tov yuav tsum raug kho raws li kev xeem (2).
C2 = C1/1+0.0011 (t1-t2)
1 + 0.0011 yog qhov nthuav coefficient ntawm glacial acetic acid
Chav tsev ntawm chav tsev kub rau T2 calibration ntawm perchloric acid yog Celsius (° C).
Chav tsev ntawm chav tsev kub uas ib qho piv txwv yog titrated yog Celsius (° C).
C1 t2 ℃ perchloric acid standard tov concentration, hauv moles ib liter (MOL / L)
3. Qhuav thiab tsis hnyav
3.1 Cov cuab yeej:
Thermostatic ziab qhov cub, 1/10,000 tshuav nyiaj li cas.
3.2 Cov txheej txheem:
Noj 1-2 gram ntawm cov qauv (qhov tseeb rau 0.5mg) thiab muab tso rau hauv lub khob ua ntej qhuav tas li thiab hnyav hnyav.Cov qauv yuav muaj cov tuab tuab nyob hauv qab ntawm lub raj mis hnyav.Raws li rau cov qauv, lawv yuav qhuav nyob rau hauv ib tug (105 ± 5 ℃) thermoelectric qhov cub kom qhov hnyav tas li.
3.3 Kev suav
X yog m rho tawm m1 faib los ntawm m rho m2 x 100
Qhov twg: X- noo noo%%
m1 - Qhov hnyav ntawm cov qauv qhuav ntxiv rau ntsuas lub raj mis g
m2 - Ntsuas qhov loj ntawm lub raj mis g
m- Qhov hnyav ntawm tus qauv g
3.4 Cov ntsiab lus rau kev mloog
1. Cov ntsiab lus tseem ceeb: 1. Cov qauv sib txuas yuav tsum tau ua kom qhuav thiab tsis hnyav.Ib qho piv txwv muaj ob qhov txiaj ntsig, thiab qhov yuam kev ntawm ob qhov txiaj ntsig tsis ntau tshaj 0.5%.
2. Qhov hnyav yuav tsum ceev faj, raug rau 0.0001.
4 rau kev sib hloov
4.1 Cov ntsiab cai
Lub dav hlau-polarized lub teeb dhau los ntawm cov kua lossis cov tshuaj ntawm cov khoom sib xyaw uas muaj qee qhov kev ua haujlwm kho qhov muag (uas yog, cov khoom siv kho qhov muag) tuaj yeem ua rau kev hloov kho qhov muag, ua rau lub dav hlau ntawm lub teeb polarized tig mus rau sab laug lossis sab xis.Tus naj npawb ntawm kev sib hloov yog hu ua optical rotation.Ntawm qhov kub thiab txias (feem ntau yog qhia los ntawm t, tuaj yeem yog 20 ℃ lossis 25 ℃) qee qhov wavelength ntawm lub teeb (daj sodium teeb nrog D, wavelength λ 589.3nm) polarized lub teeb los ntawm txhua milliliter uas muaj 1g optical kev ua ub no, thickness ntawm 1dm (uas yog, 10cm) daws thaum qhov kev sib hloov yog hu ua kev sib hloov kho qhov muag.
4.2 Kev xav tau
D kab ntawm sodium spectrum (589. 3 nm) tau siv los ntsuas lub raj ntev ntawm 1dm thiab qhov kub thiab txias yog 20 ℃.
4.3 Cov cuab yeej
4.3.1 Digital autotropy (qhov tseeb ± 0.001°)
4.3.2 Kev ntsuas hluav taws xob (qhov tseeb 0.0001g).
4.4 Txoj kev ua haujlwm
Noj 3g ntawm cov khoom no, raug rau 0.0001g, muab tso rau hauv 100ml volumetric lub raj mis, ntxiv ethanol rau yaj thiab dilute nws mus rau lub teev, co zoo.Yaug lub raj ntsuas nrog me me ntawm cov kua rau ob peb zaug, thiab tom qab ntawd maj mam txhaj cov kua rau hauv lub raj ntsuas (tsis txhob ua npuas tshwm sim), tso lub raj ntsuas hauv lub gyroscope rau kev kuaj pom, thiab nyeem cov ntawv nyeem kev hloov kho qhov muag rau 3 zaug nyob rau hauv parallel.
4.5 Kev suav
Qhov tshwj xeeb kev sib hloov ntawm L-glutamate Diethyl carbonate rau sodium spectrum D-line ntawm 20 ℃ [Alpha] D20 tau xam raws li hauv qab no:
[ɑ] D20 = alpha/m
Qhov twg: α - nruab nrab tus naj npawb ntawm cov ntsuas ntsuas ntsuas ntsuas;
m -- qauv zoo.
4.6 Kev tso cai sib txawv: Qhov sib txawv ntawm tib tus qauv yuav tsum tsis pub tshaj 0.02% thaum ntsuas ob zaug.
4.7 Kev ceev faj
4.7.1 Cov kuab tshuaj yuav tsum tau siv ua calibration calibration ua ntej txhua qhov kev ntsuas: Tom qab ntsuas, calibration yuav tsum tau ua dua los txiav txim seb pes tsawg point hloov thaum ntsuas.Yog tias muaj kev hloov pauv, qhov kev hloov kho qhov muag ntawm cov qauv yuav tsum tau txiav txim siab dua.
4.7.2 Cov qauv yuav tsum tau qhia meej tom qab kev sib cais thiab dilution;txwv tsis pub, nws yuav tsum tau lim.
Kauj Ruam 5 Kev tshwm sim
Cov khoom yuav tsum yog dawb mus rau dawb microcrystalline hmoov