L-Proline CAS 147-85-3 (H-Pro-OH) Kev Ntsuas 98.5 ~ 101.0% Lub Hoobkas Zoo

L-Proline (H-Pro-OH) (CAS: 147-85-3) AJI 97 Test Method
Kev txheeb xyuas: Sib piv cov infrared absorption spectrum ntawm cov qauv nrog cov qauv ntawm cov txheej txheem potassium bromide disc.
Kev sib hloov tshwj xeeb [α]20 / D: Cov Qauv Qhuav, C = 4, H2O
State of Solution (Transmittance): 1.0g hauv 10ml ntawm H2O, spectrophotometer, 430nm, 10mm cell thickness.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml ntawm 0.01mol / L HCl
Ammonium (NH4): B-1
Sulfate (SO4): 1.2g, (1), ref: 0.50ml ntawm 0.005mol / L H2SO4
Hlau (Fe): 1.5g, ref: 1.5ml ntawm Hlau Std.(0.01 mg/ml)
Heavy Metals (Pb): 2.0g, (1), ref: 2.0ml ntawm Pb Std.(0.01 mg/ml)
Arsenic (As2O3): 2.0g, (1), ref: 2.0ml ntawm As2O3 Std.
Lwm Yam Amino Acids: Cov Qauv Xeem: 30μg, B-6-a
Poob rau ziab: ntawm 105 ℃ rau 3 teev.
Assay: Cov qauv qhuav, 120mg, (1), 3ml ntawm formic acid, 50ml ntawm glacial acetic acid, 0.1mol / L HCLO4 1ml = 11.513mg C5H9NO2
pH Test: 1.0g hauv 10ml ntawm H2O

L-Proline (H-Pro-OH) (CAS: 147-85-3) USP35 Test Method
DEFINITION
Proline muaj NLT 98.5% thiab NMT 101.5% ntawm L-Proline (C5H9NO2), suav ntawm lub hauv paus qhuav.
IDENTIFICATION
A. INFRARED ABSORPTION <197K>
QHIA
txheej txheem
Qauv: 110 mg ntawm Proline
Tsis muaj: Sib tov 3 mL ntawm formic acid thiab 50 mL ntawm glacial acetic acid.
Titrimetric system
(Saib Titrimetry <541>)
Hom: Direct titration
Titrant: 0.1 N perchloric acid VS
Endpoint detection: Potentiometric
Kev Ntsuas: Dissolve cov qauv hauv 3 mL ntawm formic acid thiab 50mL ntawm glacial acetic acid.Titrate nrog Titrant.Ua qhov kev txiav txim siab Blank.
Xam qhov feem pua ​​​​ntawm Proline (C5H9NO2) hauv cov qauv coj mus kuaj:
Result = {[(VS-VB) x N x F]/W} x100
VS = ntim ntawm Titrant noj los ntawm Sample (mL)
VB = ntim ntawm Titrant noj los ntawm Blank (mL)
N = qhov tseeb normality ntawm Titrant (mEq / mL)
F = equivalency factor, 115.1 mg/mEq
W = Sample nyhav (mg)
Kev lees txais: 98.5% ~ 101.5% ntawm lub hauv paus qhuav
IMPURITIES
RESIDUEON ON IGNITION <281>: NMT 0.4%
chloride thiab sulfate, chloride <221>
Standard tov: 0.50mL ntawm 0.020 N hydrochloric acid
Qauv: 0.73g ntawm Proline
Kev lees txais: NMT 0.05%
chloride thiab sulfate, sulfate <221>
Standard tov: 0.10mL ntawm 0.020 N sulfuric acid
Qauv: 0.33g ntawm Proline
Kev lees txais: NMT 0.03%
Hlau <241>: NMT 30ppm
HEAVY METALS, Method I <231>: NMT 15ppm
TXOJ CAI TSHIAB
Kev daws teeb meem tsim nyog: 0.4mg / mL txhua ntawm USP L-Proline RS thiab USP L-Threonine RS hauv 0.1 N hydrochloric acid
Kev daws teeb meem: 0.05mg / mL ntawm USP L-Proline RS hauv 0.1 N hydrochloric acid.[CEEB TOOM-Qhov kev daws teeb meem no muaj qhov sib npaug sib npaug rau 0.5% ntawm cov qauv tshuaj.]
Cov qauv tshuaj: 10mg / mL ntawm Proline hauv 0.1 N hydrochloric acid
Chromatographic system
(Saib Chromatography <621>, Thin-Layer Chromatography.)
Mode: TLC
Adsorbent: 0.25-mm txheej ntawm chromatographic silica gel sib tov
Daim ntawv ntim: 5μL
Tsim cov kuab tshuaj: Butyl cawv, glacial acetic acid, thiab dej (3: 1: 1)
Txau reagent: 2 mg / mL ntawm ninhydrin hauv kev sib xyaw ntawm butyl cawv thiab 2N acetic acid (95: 5)
Qhov system haum
Kev xav tau kev tsim nyog: Lub chromatogram ntawm qhov kev daws teeb meem tsim nyog nthuav tawm ob qhov sib cais kom meej.
Kev tsom xam
Cov Qauv: Cov kev daws teeb meem tsim nyog, Cov qauv kev daws teeb meem, thiab cov qauv kev daws teeb meem.
Tom qab cua qhuav lub phaj, tshuaj tsuag nrog tshuaj tsuag reagent, thiab cua sov ntawm 100 ° thiab 105 ° rau 15 min.Tshawb xyuas lub phaj hauv qab lub teeb dawb.
Kev lees txais cov qauv: Txhua qhov chaw thib ob ntawm cov qauv kev daws teeb meem tsis loj dua lossis hnyav dua li qhov chaw tseem ceeb ntawm Cov Txheej Txheem Kev daws teeb meem.
Tus kheej impurities: NMT 0.5%
Tag nrho impurities: NMT 2.0%
TSEEM CEEB
OPTICAL ROTATION, Kev sib hloov tshwj xeeb <781S>
Sample tov: 40 mg / mL hauv dej
Kev lees txais cov qauv: -84.3 ° txog -86.3 °
LOSSON DRYING <731>: Qhuav ib qho qauv ntawm 105 ℃ rau 3 h: nws poob NMT 0.4% ntawm nws qhov hnyav.
TSEEM CEEB
PACKAGING THIAB STORAGE: Khaws rau hauv cov thawv kaw kom zoo.
USP REFERENCE STANDARDS <11>
USP L-Proline RS UAS
USP L-Threonine RS