L-(-)-Tyrosine CAS 60-18-4 (H-Tyr-OH) Assay 98.5 ~ 101.5% Hoobkas Zoo

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) AJI 97 Test Method
Kev txheeb xyuas: Sib piv cov infrared absorption spectrum ntawm cov qauv nrog cov qauv ntawm cov txheej txheem potassium bromide disc.
Kev sib hloov tshwj xeeb [α]20 / D: Cov qauv qhuav, C = 5, 1mol / L HCl
Lub xeev ntawm kev daws teeb meem (Transmittance): 0.5g hauv 10ml ntawm 1mol / L HCl, spectrophotometer, 430nm, 10mm cell thickness.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml ntawm 0.01mol / L HCl
Ammonium (NH4): B-2
Sulfate (SO4): 1.2g, (1), ref: 0.50ml ntawm 0.005mol / L H2SO4
Hlau (Fe): 1.5g, (2), ref: 1.5ml ntawm Hlau Std.(0.01 mg/ml)
Heavy Metals (Pb): 2.0g, (2), ref: 2.0ml ntawm Pb Std.(0.01 mg/ml)
Arsenic (As2O3): 2.0g, (2), ref: 2.0ml ntawm As2O3 Std.
Lwm Yam Amino Acids: Dilute ammonia R2 Test Sample: 50μg, F-6-a, tswj: L-Tyr 0.25μg
Poob rau ziab: ntawm 105 ℃ rau 3 teev.
Residue ntawm Ignition (Sulfated): AJI Test 13
Assay: Cov qauv qhuav, 180mg, (1), 6ml ntawm formic acid, 50ml ntawm glacial acetic acid, 0.1mol / L HCLO4 1ml = 18.119mg C9H11NO3
pH Test: 0.02g hauv 100ml saturated aqueous tov

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) USP35 Test Method
DEFINITION
Tyrosine muaj NLT 98.5% thiab NMT 101.5% ntawm L-Tyrosine (C9H11NO3), suav ntawm lub hauv paus qhuav.
IDENTIFICATION
A. INFRARED ABSORPTION <197K>
QHIA
txheej txheem
Qauv: 180 mg ntawm Tyrosine
Tsis muaj: Sib tov 6 mL ntawm formic acid thiab 50 mL ntawm glacial acetic acid.
Titrimetric system
(Saib Titrimetry <541>)
Hom: Direct titration
Titrant: 0.1 N perchloric acid VS
Endpoint detection: Potentiometric
Kev Ntsuam Xyuas: Dissolve tus qauv hauv 6 mL ntawm formic acid thiab 50mL ntawm glacial acetic acid, thiab titrate nrog Titrant.Ua qhov kev txiav txim siab Blank.
Xam qhov feem pua ​​​​ntawm tyrosine (C9H11NO3) hauv cov qauv coj mus kuaj:
Result = {[(VS-VB) x N x F]/W} x100
VS = ntim ntawm Titrant noj los ntawm Sample (mL)
VB = ntim ntawm Titrant noj los ntawm Blank (mL)
N = qhov tseeb normality ntawm Titrant (mEq / mL)
F = equivalency factor, 181.2 mg/mEq
W = Sample nyhav (mg)
Kev lees txais: 98.5% ~ 101.5% ntawm lub hauv paus qhuav
IMPURITIES
RESIDUEON IGNITION <281>: NMT 0.4%
chloride thiab sulfate, chloride <221>
Standard tov: 0.20mL ntawm 0.020 N hydrochloric acid
Qauv: 0.35 g ntawm Tyrosine.[CEEB TOOM-Yog tias tsim nyog, yaj tus qauv los ntawm cua sov kom ze rau boiling thiab ntxiv 1 mL ntawm nitric acid.]
Kev lees txais: NMT 0.04%
chloride thiab sulfate, sulfate <221>
Standard tov: 0.50mL ntawm 0.020 N sulfuric acid
Qauv: 1.2 g ntawm Tyrosine.[CEEB TOOM-Yog tias tsim nyog, yaj tus qauv los ntawm kev ntxiv 6 mL ntawm diluted hydrochloric acid.]
Kev lees txais: NMT 0.04%
IRON <241>: NMT 30ppm [CEEB TOOM-Yog tias tsim nyog, siv 2 mL ntawm hydrochloric acid kom yaj cov qauv.]
HEAVY METALS, Method I <231>: NMT 15ppm
TXOJ CAI TSHIAB
Diluted ammonia daws: Dilute 16 mL ntawm concentrated ammonia nrog dej rau 100 mL.
Kev daws teeb meem tsim nyog: Dissolve 10 mg ntawm USP L-Tyrosine RS thiab 10 mg ntawm USP L-Phenylalanine RS hauv 1 mL ntawm Diluted ammonia tov, thiab dilute nrog dej mus rau 25.0 mL.
Kev daws teeb meem: Hauv ib lub hwj uas tsim nyog, yaj ib qho ntawm USP L-Tyrosine RS hauv 1 mL ntawm Diluted ammonia tov.Dilute nrog dej rau ntim kom tau txais kev daws ntawm 0.05mg / mL.[CEEB TOOM-Qhov kev daws teeb meem no muaj qhov sib npaug sib npaug rau 0.5% ntawm cov qauv tshuaj.]
Cov qauv kev daws teeb meem: Hloov 0.1 g ntawm Tyrosine rau hauv 10-mL ntim ntim, ntxiv cov tshuaj ammonia txaus kom yaj, thiab dilute nrog dej rau ntim.
Chromatographic system (Saib Chromatography <621>, Thin-Layer Chromatography.)
Mode: TLC
Adsorbent: 0.25-mm txheej ntawm chromatographic silica gel sib tov
Daim ntawv ntim: 5μL
Tsim cov kuab tshuaj: Isopropyl cawv thiab ammonium hydroxide (7: 3)
Txau reagent: 2 mg / mL ntawm ninhydrin hauv kev sib xyaw ntawm butyl cawv thiab 2N acetic acid (95: 5)
Qhov system haum
Kev xav tau kev tsim nyog: Lub chromatogram ntawm qhov kev daws teeb meem tsim nyog nthuav tawm ob qhov sib cais kom meej.
Kev tsom xam
Cov Qauv: Cov kev daws teeb meem tsim nyog, Cov qauv kev daws teeb meem, thiab cov qauv kev daws teeb meem.
Qhuav lub phaj ntawm 100 ° thiab 105 ° kom txog thaum ammonia ploj tag.Txau nrog Spray reagent, thiab kub ntawm 100 ° thiab 105 ° rau 15 min.Tshawb xyuas lub phaj hauv qab lub teeb dawb.
Kev lees txais cov qauv: Txhua qhov chaw thib ob ntawm cov qauv kev daws teeb meem tsis loj dua lossis hnyav dua li qhov chaw tseem ceeb ntawm Cov Txheej Txheem Kev daws teeb meem.
Txhua tus neeg impurities: NMT 0.5%
Tag nrho impurities: NMT 2.0%
TSEEM CEEB
OPTICAL ROTATION, Kev sib hloov tshwj xeeb <781S>
Cov qauv tshuaj: 50 mg / mL hauv 1 N hydrochloric acid
Kev lees txais cov qauv: -9.8 ° txog -11.2 °
LOSSON RAU DRYING <731>: Qhuav ib qho qauv ntawm 105 ℃ rau 3 h: nws poob NMT 0.3% ntawm nws qhov hnyav.
TSEEM CEEB
PACKAGING THIAB STORAGE: Khaws rau hauv cov thawv kaw kom zoo.
USP REFERENCE STANDARDS <11>
USP L-Phenylalanine RS
USP L-Tyrosine RS