L-Valine CAS 72-18-4 (H-Val-OH) Kev Ntsuas 98.5 ~ 101.0% Lub Hoobkas Zoo

L-Valine (H-Val-OH) (CAS: 72-18-4) AJI 97 Test Method
Kev txheeb xyuas: Sib piv cov infrared absorption spectrum ntawm cov qauv nrog cov qauv ntawm cov txheej txheem potassium bromide disc.
Kev sib hloov tshwj xeeb [α]20 / D: Cov Qauv Qhuav, C = 8, 6mol / L HCl
State of Solution (Transmittance): 0.5g hauv 20ml ntawm H2O, spectrophotometer, 430nm, 10mm cell thickness.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml ntawm 0.01mol / L HCl
Ammonium (NH4): B-2
Sulfate (SO4): 1.2g, (1), ref: 0.50ml ntawm 0.005mol / L H2SO4
Hlau (Fe): 1.5g, (2), ref: 1.5ml ntawm Hlau Std.(0.01 mg/ml)
Heavy Metals (Pb): 2.0g, (2), ref: 2.0ml ntawm Pb Std.(0.01 mg/ml)
Arsenic (As2O3): 2.0g, (2), ref: 2.0ml ntawm As2O3 Std.
Lwm Yam Amino Acids: Dilute ammonia R2 Test Sample: 50μg, F-6-a, tswj: L-Tyr 0.25μg
Poob rau ziab: ntawm 105 ℃ rau 3 teev.
Residue ntawm Ignition (Sulfated): AJI Test 13
Assay: Cov qauv qhuav, 120mg, (1), 3ml ntawm formic acid, 50ml ntawm glacial acetic acid, 0.1mol / L HCLO4 1ml = 11.715mg C5H11NO2
pH Test: 1.0g hauv 20ml ntawm H2O

L-Valine (H-Val-OH) (CAS: 72-18-4)JP16 Test Method
L-Valine, thaum qhuav, muaj tsawg dua 98.5% ntawm C5H11NO2.
Nqe lus piav qhia L-Valine tshwm sim raws li cov muaju dawb lossis crystalline hmoov.Nws yog odorless los yog muaj ib tug faint yam ntxwv tsw, thiab muaj ib tug me ntsis qab zib saj, uas yuav iab.
Nws yog dawb do soluble nyob rau hauv formic acid, soluble nyob rau hauv dej, thiab xyaum insoluble nyob rau hauv ethanol (95).
Nws dissolves nyob rau hauv dilute hydrochloric acid.
Kev txheeb xyuas Kev txheeb xyuas qhov infrared absorption spectrum ntawm L-Valine, yav tas los qhuav, raws li qhia nyob rau hauv cov poov tshuaj bromide disk txoj kev nyob rau hauv Infrared Spectrophotometry <2.25>, thiab muab piv cov spectrum nrog cov Reference Spectrum: ob qho tib si spectra qhia zoo sib xws ntawm kev nqus ntawm tib yoj tooj. .
Kev sib hloov kho qhov muag <2.49> [a]20D: +26.5 ° ~ + 29.0 ° (tom qab ziab, 2 g, 6 mol / L hydrochloric acid TS, 25 mL, 100 mm).
pH <2.54> Dissolve 0.5 g ntawm L-Valine hauv 20 mL dej: pH ntawm cov tshuaj no yog nyob nruab nrab ntawm 5.5 thiab 6.5.
Purity (1) Clarity thiab xim ntawm kev daws Dissolve 0.5 g ntawm L-Valine hauv 20 mL dej: cov tshuaj yog ntshiab thiab tsis muaj xim.
(2) Chloride <1.03>- Ua qhov kev sim nrog 0.5 g ntawm L-Valine.Npaj cov tshuaj tiv thaiv nrog 0.30 mL ntawm 0.01mol / L hydrochloric acid VS (tsis ntau tshaj 0.021%).
(3) Sulfate <1.14>- Ua qhov ntsuas nrog 0.6 g ntawm L-Valine.Npaj cov tshuaj tswj nrog 0.35 mL ntawm 0.005mol / L sulfuric acid VS (tsis ntau tshaj 0.028%).
(4) Ammonium <1.02>-Ua qhov ntsuas nrog 0.25 g ntawm L-Valine.Npaj cov tshuaj tswj nrog 5.0 mL ntawm Standard Ammonium Solution (tsis ntau tshaj 0.02%).
(5) Cov hlau hnyav <1.07>-Tau nrog 1.0 g ntawm L-Valine raws li Txoj Kev 1, thiab ua qhov kev sim.Npaj cov tshuaj tswj nrog 2.0 mL ntawm Standard Lead Solution (tsis ntau tshaj 20 ppm).
(6) Arsenic <1.11>-Tau nrog 1.0g ntawm L-Valine, npaj cov tshuaj ntsuam xyuas raws li Txoj Kev 2, thiab ua qhov kev sim (tsis ntau tshaj 2 ppm).
(7) Lwm yam tshuaj - Dissolve 0.10 g ntawm L-Valine nyob rau hauv 25mL dej, thiab siv cov tshuaj no raws li cov qauv tov.Pipet 1 mL ntawm cov qauv tov, thiab ntxiv dej kom raws nraim 50 mL.Pipet 5 mL ntawm cov tshuaj no, ntxiv dej kom raws nraim 20 mL, thiab siv cov tshuaj no raws li tus qauv tov.Ua qhov kev sim nrog cov kev daws teeb meem no raws li qhia hauv Thin-txheej Chromatography <2.03>.Spot 5mL txhua yam ntawm cov qauv kev daws teeb meem thiab cov qauv kev daws teeb meem ntawm lub phaj ntawm silica gel rau nyias txheej chromatography.Tsim lub phaj nrog kev sib xyaw ntawm 1-butanol, dej thiab acetic acid (100) (3: 1: 1) kom deb li deb ntawm 10 cm, thiab qhuav lub phaj ntawm 80 ℃ rau 30 feeb.Txau ib qho kev daws teeb meem ntawm ninhydrin hauv acetone (1 hauv 50) ntawm lub phaj, thiab cua sov ntawm 80 ℃ rau 5 feeb: qhov chaw uas tsis yog qhov chaw tseem ceeb ntawm cov qauv tshuaj tsis yog khaus ntau dua li qhov chaw los ntawm cov txheej txheem kev daws teeb meem.
Poob ntawm ziab <2.41> Tsis ntau tshaj 0.30% (1 g, 105 ℃, 3 teev).
Residue ntawm ignition <2.44> Tsis ntau tshaj 0.1% (1 g).
Kev ntsuas qhov hnyav yog kwv yees li 0.12 g ntawm L-Valine, yav tas los qhuav, thiab yaj hauv 3 mL ntawm formic acid, ntxiv 50 mL ntawm acetic acid (100), thiab titrate <2.50> nrog 0.1 mol / L perchloric acid VS (potentiometric titration) .Ua ib qho kev txiav txim siab dawb paug, thiab ua ib qho kev kho uas tsim nyog.
Txhua mL ntawm 0.1 mol / L perchloric acid VS ＝11.72 mg ntawm C5H11NO2
Containers thiab cia Containers-Tight containers.